Ppp2r1b (NM_001034085) Mouse Tagged ORF Clone

CAT#: MG219372

Ppp2r1b (GFP-tagged) - Mouse protein phosphatase 2 (formerly 2A) regulatory subunit A (PR 65) beta isoform (Ppp2r1b) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppp2r1b
• Synonyms: 2410091N08Rik; AI790395
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219372 representing NM_001034085
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCAGCAGGGCCAGGGTCCGGTCCGGGGGCAGCGGGTGGAGATGGAGACGATTCGCTCTACC
  CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAGGACGTGCAGCTCCGTCTCAACAGTATTAAGAAGTT
  ATCAACTATAGCTCTAGCACTCGGGGTAGAAAGGACTCGAACGGAACTGCTGCCTTTCCTTACAGATACT
  ATTTATGATGAAGATGAGGTGCTGTTAGCTCTCGCCGAGCAGCTGGGAAATTTCACTGGTTTGGTGGGCG
  GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAGTTTGGCTACGGTGGAGGAGACTGTCGTTCG
  AGACAAGGCTGTGGAGTCGCTGAGGCAGATCTCGCAGGAGCACACTCCTGTTGCCCTTGAAGCTCATTTT
  GTGCCTCTGGTGAAGCGCCTGGCAAGCGGGGACTGGTTCACGTCGCGCACGTCTGCATGTGGCTTGTTCA
  GCGTTTGCTATCCCAGAGCTTCAAATGCTGTCAAGGCAGAGATTCGACAGCACTTCCGTTCTCTCTGCTC
  GGATGATACACCAATGGTGCGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA
  GACAGTGTGAAAACTGAAATTGTCCCATTGTTCACGAATTTGGCTTCAGATGAACAGGACTCTGTGCGGC
  TGCTAGCTGTGGAAGCCTGCGTCAGCATTGCCCAGTTACTGTCTCAGGAGGACCTGGAAGCCTTGGTAAT
  GCCTACACTTCGACAAGCAGCAGAAGATAAGTCCTGGCGGGTTCGATATATGGTGGCTGACAAGTTTTCA
  GAGCTCCAGAAAGCGGTGGGCCCTAAGATTGCACTCAGCGACCTCATCCCCGCCTTCCAGAGCCTCCTCC
  GAGACTGCGAAGCTGAAGTCCGAGCAGCCGCTGCCCACAAAGTAAGAGAGCTTTGTGAGAACCTGCCCGC
  TGAAGGTAGAGAGACTGTAATTATGAATCAAATCCTGCCCTATATAAAGGAATTAGTGTCTGATACCAAC
  CAACATGTCAAGTCAGCGCTAGCTTCTGTAATTATGGGATTATCTACAGTTTTGGGCAAAGAGAATACCA
  TTGAACATCTTCTGCCTCTATTTTTAGCTCAGTTAAAGGACGAGTGTCCAGAAGTTCGTTTAAATATCAT
  CTCCAATTTGGATTGTGTGAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCGGCCATA
  GTGGAACTGGCTGAAGATGCCAAGTGGAGGGTCCGCCTGGCTATCATTGAGTACATGCCGCTGCTGGCTG
  GCCAGCTGGGTGTGGAGTTCTTTGATGAAAAACTCAATTCTTTGTGTATGGCCTGGCTTGTGGATCATGT
  CTATGCCATTCGGGAGGCAGCCACCAACAACCTCATGAAGTTAGTCCAGAAGTTCGGTACAGAGTGGGCC
  CAGAATACCATTGTCCCTAAAGTGTTGGTAATGGCAAATGACCCTAACTACTTACACAGAATGACCACGC
  TCTTCTGCATTAATGCACTGTCTGAAGCCTGTGGTAAGGAAATAACCACTAAGCAAATGTTGCCCATCGT
  GTTGAAAATGGCAGGAGACCAGGTAGCAAATGTCCGCTTCAATGTGGCCAAATCACTGCAGAAAATTGGA
  CCGATTCTAGACACCAACGCCTTGCAGGGGGAAGTGAAGCCGGTGCTGCAGAAATTGGGTCAGGACGAAG
  ACATGGATGTCAAATATTTTGCACAGGAAGCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGATTT
  TCCTGTGAAGGACAGTGAAGAGCCCAGCATCCCTGGGGTTGACAAGAATCTCTTCCTGAGACCCCGAGGG
  CCTGGAGAGGACACTGGGAAGTTTCTACCTTTGATGTCTAGGCACAGAGAGACGGAGCGGCTCCAGTGGC
  TTCCGAGGAGGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219372 representing NM_001034085
  Red=Cloning site Green=Tags(s)
  
  MAGAAGPGSGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRTELLPFLTDT
  IYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLESLATVEETVVRDKAVESLRQISQEHTPVALEAHF
  VPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQHFRSLCSDDTPMVRRAAASKLGEFAKVLEL
  DSVKTEIVPLFTNLASDEQDSVRLLAVEACVSIAQLLSQEDLEALVMPTLRQAAEDKSWRVRYMVADKFS
  ELQKAVGPKIALSDLIPAFQSLLRDCEAEVRAAAAHKVRELCENLPAEGRETVIMNQILPYIKELVSDTN
  QHVKSALASVIMGLSTVLGKENTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAI
  VELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWA
  QNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGKEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIG
  PILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLDFPVKDSEEPSIPGVDKNLFLRPRG
  PGEDTGKFLPLMSRHRETERLQWLPRRQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034085
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034085.1, NP_001029257.1
• RefSeq Size: 3636 bp
• RefSeq ORF: 1977 bp
• Locus ID: 73699
• Cytogenetics: 9 A5.3