Mid1 (NM_183151) Mouse Tagged ORF Clone

CAT#: MG220976

Mid1 (GFP-tagged) - Mouse midline 1 (Mid1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mid1
• Synonyms: 61B3-R; DXHXS1141; Fxy; Trim18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220976 representing NM_183151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC
  CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC
  TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA
  GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATCATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC
  CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCAACACCATGTCCTCTGCCGAGAAGGT
  TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC
  TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
  CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGGTGAATATGTACTG
  TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA
  GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTGGAGAGTAATCTCACCAATCTTATTAAGAGAA
  ACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAAGTCAATGCATCCCG
  TCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAATCATTCAGCAACGAAGACAAATTATT
  GGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTTAGCTCAGCAGATTGCAAACTGTAAAC
  AGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCACTCGCTGAAGGAAAATGACCACGCCCG
  TTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTCCTAATT
  CCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGATTTTTCCCGGGAGAAGAAACTGCTAG
  AATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGAGAAGAGCTCTGCACCGCTTCCTACGA
  CACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA
  TTCACCGGACAAGCCAATGTTGTCAACGTGGCCTGTGACGGCACCTGTCTCCTCGGCTCCGCAGGTCTGT
  GTAACTCGGCGGACAGCTGGATGATCGTGCCCAACATCAAGCAGAACCACTACACCGTGCACGGCCTGCA
  AAGTGGCACCAAGTATATCTTCACGGTGAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGA
  AAGCTGAAGACCAACAGTCAGCCGTTTAGACTGGATCCCAAATCGGCTCATCGCAAGCTGAAGGTGTCCC
  ACGACAACCTGACTGTCGAGCGCGACGAGTCGTCCTCTAAGAAGAGTCACGCGCCGGAGCGCTTCGCTGG
  TCAGGGGAGCTACGGAGTGGCTGGCAACGTGTTCATCGACAGCGGCCGTCACTACTGGGAAGTGGTCACC
  AGCGGAAGCACATGGTACGCCATCGGCCTGGCGTACAGATCGGCGCCGAAACACGAGTGGATCGGGAAGA
  ACGCGGCGTCCTGGGCCCTCTGCCGCTGCCACAACCACTGGGCGGTGCGACACGACGGCAAGGAGACCCC
  CATCGCGCCGGCCCCTCACCTCAGGCGCGTCGGCGTCCTGCTGGACTACGACAACGGCTCCATCGCCTTC
  TACGACGCTCTGAGCTCCGTCCACCTCCACACCTTCCACGCGGCGCTCGCGCAGCCCGTGTGCCCCACCT
  TCACCGTGTGGAACAAGTGTCTGACCATCGTCACGGGTCTGCCCATCCCGGACCATCTGGACTGTACGGA
  GCAGCGACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220976 representing NM_183151
  Red=Cloning site Green=Tags(s)
  
  METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGL
  DGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVS
  YCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVA
  ALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQII
  GTKIKEGKVIRLRKLAQQIANCKQCLERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLI
  PEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPAIREELCTASYDTITVHWTSEDEFSVVSYELQYTI
  FTGQANVVNVACDGTCLLGSAGLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFTVKAINQAGSRSSEPG
  KLKTNSQPFRLDPKSAHRKLKVSHDNLTVERDESSSKKSHAPERFAGQGSYGVAGNVFIDSGRHYWEVVT
  SGSTWYAIGLAYRSAPKHEWIGKNAASWALCRCHNHWAVRHDGKETPIAPAPHLRRVGVLLDYDNGSIAF
  YDALSSVHLHTFHAALAQPVCPTFTVWNKCLTIVTGLPIPDHLDCTEQRP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183151
• ORF Size: 2040 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_183151.1, NP_898974.1
• RefSeq Size: 3974
• RefSeq ORF: 2043
• Locus ID: 17318