Btc (NM_007568) Mouse Tagged ORF Clone

CAT#: MG221004

Btc (GFP-tagged) - Mouse betacellulin epidermal growth factor family member (Btc), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Btc
• Synonyms: Bcn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221004 representing NM_007568
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCAACAGCCCCGGGTAGCAGTGTCAGCTCCCTGCCGCTGCTCCTGGTCCTTGCCCTGGGTCTTG
  CAATTCTCCACTGTGTGGTAGCAGATGGGAACACAACCAGAACACCAGAAACCAATGGCTCTCTTTGTGG
  AGCTCCTGGGGAAAACTGCACAGGTACCACCCCTAGACAGAAAGTGAAAACCCACTTCTCTCGGTGCCCC
  AAGCAGTACAAGCATTACTGCATCCATGGGAGATGCCGCTTCGTGGTGGACGAGCAAACTCCCTCCTGCA
  TCTGTGAGAAAGGCTACTTTGGGGCTCGGTGTGAGCGAGTGGACCTGTTTTACCTCCAGCAGGACCGGGG
  GCAGATCCTGGTGGTCTGCTTGATAGTGGTCATGGTGGTGTTCATCATTTTAGTCATCGGCGTCTGCACC
  TGCTGTCATCCTCTTCGGAAACATCGTAAAAAAAAGAAGGAAGAGAAAATGGAGACTTTGGATAAAGATA
  AAACTCCCATAAGTGAAGATATTCAAGAGACCAATATTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221004 representing NM_007568
  Red=Cloning site Green=Tags(s)
  
  MDPTAPGSSVSSLPLLLVLALGLAILHCVVADGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCP
  KQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFYLQQDRGQILVVCLIVVMVVFIILVIGVCT
  CCHPLRKHRKKKKEEKMETLDKDKTPISEDIQETNIA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007568
• ORF Size: 531 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007568.5, NP_031594.1
• RefSeq Size: 2876
• RefSeq ORF: 534
• Locus ID: 12223
• Gene Summary: This gene encodes a member of the epidermal growth factor (EGF) family. These growth factors are ligands for the EGFR/ErbB receptor tyrosine kinases, and play roles in cell growth and differentiation. The encoded protein is synthesized as a transmembrane precursor that is proteolytically cleaved to generate a mature peptide, and plays a role in the differentiation of pancreatic beta cells. This gene may also play a protective role in acute pancreatitis, whereas increased expression of this gene may contribute to diabetic macular edema. Gene therapy using combinations of this gene and other pancreas-specific transcription factors may induce islet neogenesis and remediate hyperglycemia in type 1 diabetes. [provided by RefSeq, Apr 2011]