Cnot1 (NM_153164) Mouse Tagged ORF Clone

CAT#: MG221036

Cnot1 (GFP-tagged) - Mouse CCR4-NOT transcription complex subunit 1 (Cnot1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cnot1
• Synonyms: 6030411K04Rik; AA815922; D830048B13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221036 representing NM_153164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA
  ACTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT
  ATTACGCTGCCTATTTTCACATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG
  ACACAGTTTCTGATTCAGGAGTGTGCCTCGCTGATTACAAAGCCAAATTTTATCTCAACATTGTCATATG
  CCATTGATAATCCATTGCACTACCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAA
  AGTTCTCAAGCTAAGTAAAGTACAGGAGGTCATTTTTGGTCTTGCCCTTTTGAATTCTTCTAGCCCAGAT
  CTCAGGGGTTTTGCTGCCCAGTTTATTAAACAGAAGCTTCCGGATCTTCTGCGTTCTTACATTGACGCAG
  ACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTACTCTCCCA
  TCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAGGAACAGATAGACGCCTTTCTTAAGACACTA
  CGCAGAGATTTTCCCCAAGAGCGCTGTCCTGTGGTGCTTGCACCACTTTTATACCCTGAAAAACGGGACA
  TTCTAATGGACAGGATTTTGCCTGATTCTGGAGGGGTAGCTAAAACCATGATGGAGAGCTCGCTAGCTGA
  CTTCATGCAGGAAGTGGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCATGCAGTTT
  GGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCCAGGGTTTTGGGAATGATGGCTCGGACTCATTCAGGAT
  TAACAGACGGTATTCCATTACAGAGCATCTCTGCTCCTGGCAGTGGGATCTGGAGCGACGGAAAAGATAA
  AAGCGAGGGGGCACAGGCACACACGTGGAATGTCGAGGTTTTGATTGATGTTCTTAAAGAGCTGAACCCG
  AGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAGATTCGTGACAGCAAAGGAC
  TTCACAATGTGGTTTATGGCATTCAAAGGGGTCTGGGCATGGAAGTGTTTCCAGTAGATTTTATATATAG
  GCCTTGGAAACATGCAGAAGGCCAGCTCTCCTTCATCCAGCATTCCCTGATAAACCCAGAGGTCTTCTGT
  TTTGCTGACTACCCCTGTCACACTGTTGCCACTGATATTTTGAAAGCACCACCAGAGGATGACAATCGAG
  AAATTGCCACATGGAAAAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGA
  GCAAGTGAAACAACTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGTTGGTATTGGCCTTACTTCAA
  ATCAACACTTCCTGGCACACCCTACGCCATGAACTCATCTCTACTTTGATGCCAATTTTCCTTGGAAACC
  ATCCTAACTCTGCCATTATTTTGCACTATGCATGGCATGGGCAGGGGCAGTCTCCATCAATTCGCCAACT
  TATAATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATA
  CTTGACGTGGCCCAGGACTTGAAGGCCCTGTCGATGCTGCTGAACGGCACTCCATTTGCTTTTGTCATTG
  ACCTTGCTGCACTCGCTTCACGGCGGGAATACCTCAAACTTGACAAGTGGCTAACAGATAAGATAAGAGA
  GCATGGGGAGCCTTTTATACAAGCATGTATGACTTTTTTAAAGAGAAGGTGCCCTTCTATTTTGGGTGGA
  CTTGCCCCAGAGAAAGATCAGCCCAAAAGTGCTCAACTTCCCGCTGAAACTCTGGCAACAATGTTGGCTT
  GTCTGCAGGCTTGTGCAGGGAGTGTTTCTCAAGAGTTATCAGAGACCATCCTCACCATGGTAGCCAATTG
  CAGTAATGTAATGAATAAGGCCAGACAGCCACCACCTGGAGTTATGCCAAAAGGTCGCCCTCCTAGTGCT
  AGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGGCATCTCTTAGTATAGGTG
  GTTCGGCTGCCCCTCACACCCAGAGCATGCAGGGTTTTCCTCCAAATCTGGGTTCTGCGTTCAGTACCCC
  TCAGTCACCAGCAAAAGCATTTCCACCACTTTCAACCCCAAATCAAACAACGGCCTTCAGTGGGATTGGA
  GGACTCTCCTCACAGCTTCCAGTAGGTGGTCTTGGCACAGGAAGCCTGACTGGCATAGGAACTGGGGCCC
  TTGGACTTCCTGCAGTGAACAATGATCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTCAACCAGCC
  TACATTCCAGCAGACCGACTTGTCTCAGGTGTGGCCAGAGGCAAATCAGCACTTTAGTAAAGAAATTGAT
  GATGAAGCAAACAGTTATTTCCAGCGAATATATAATCACCCACCACATCCAACCATGTCTGTTGATGAGG
  TATTAGAAATGCTACAGAGATTTAAAGACTCTACAATAAAAAGGGAACGAGAAGTATTTAACTGTATGCT
  GAGGAACTTGTTTGAAGAATACCGGTTTTTCCCTCAGTACCCTGATAAAGAATTACATATAACAGCTTGC
  TTGTTTGGTGGCATAATTGAAAAAGGACTGGTCACTTACATGGCATTGGGTCTGGCCCTGCGATATGTTC
  TTGAAGCCTTACGGAAGCCTTTTGGATCCAAAATGTATTATTTTGGTATTGCTGCACTAGATAGATTTAA
  AAATAGATTGAAGGACTATCCTCAGTATTGTCAGCACCTGGCTTCTATCAGCCACTTTATGCAGTTCCCA
  CATCATTTGCAGGAGTACATTGAGTACGGACAGCAGTCTCGAGATCCTCCTGTGAAAATGCAGGGCTCTA
  TTACTACCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCT
  TGCCGGTCAGGTTAACACAATGGTAACCACCTCAACAACCACCACTGTTGCTAAAACGGTTACAGTCACC
  AAGCCAACGGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATAAATACAACAAATATCGATACATTGC
  TTGTGGCCACAGATCAAACTGAGAGGATTGTGGAGCCCCCAGAAAATATTCAAGAGAAAATTGCTTTTAT
  TTTCAACAATCTCTCCCAGTCAAATATGACACAGAAGGTTGAAGAGCTTAAAGAAACTGTGAAGGAAGAA
  TTTATGCCTTGGGTTTCCCAGTATCTGGTTATGAAAAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGT
  ATTCAAACTTTCTTGATACATTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAAACCTACAGAAA
  CATTAAAGTGCTGCTGACTTCTGATAAAGCTGCGGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTG
  GGACATTGGTTAGGAATGATCACATTAGCTAAAAATAAACCCATCTTACACACAGACTTGGATGTGAAAT
  CTTTGCTGTTAGAGGCTTACGTTAAAGGACAGCAAGAGTTGCTCTATGTGGTGCCCTTTGTTGCCAAAGT
  TCTAGAGTCCAGCATTCGCAGCCTGGTTTTTAGACCACCAAACCCCTGGACAATGGCAATTATGAATGTA
  TTGGCTGAGCTGCATCAGGAGCATGACTTAAAGTTAAACCTGAAGTTTGAGATTGAGGTGCTCTGTAAGA
  ACCTTGCATTAGACATCAATGAGCTCAAACCTGGAAACCTGCTGAAAGATAAAGATCGTCTGAAGAACTT
  AGATGAGCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCCGAAGAGCTTCCTGCCATCACAACAACA
  ACAACTTCTACAACACCAGCTACCAGTACTACGTGTACAGCCACAGTTCCACCACAGCCACAGTACAGCT
  ACCACGACATCAATGTCTACTCTCTAGCAGGCCTGGCACCTCATATTACTCTAAACCCTACAATCCCTTT
  ATTTCAAGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTC
  CATCCTGTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTG
  CCCTGGATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAAT
  GGCTATGATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCA
  GCCCTGCGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACA
  ATTGTGAATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAG
  ATTGGCAACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTC
  TTAACCTACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGC
  TAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTAC
  AGGGTTTTTAGCTCAGCCCATGAAGCAAGCTTGGGCAACTGATGATGTAGCGCAGATTTATGATAAGTGT
  ATTACAGAACTGGAGCAGCACCTACACGCCATTCCACCCACTCTGGCCATGAATCCTCAAGCCCAGGCTC
  TCCGAAGTCTTTTAGAGGTTGTAGTGTTGTCTCGGAATTCTCGGGATGCGATAGCTGCTCTTGGATTACT
  ACAGAAGGCTGTAGAAGGCTTATTAGATGCCACAAGTGGTGCTGACGCCGACTTACTGCTGCGATACAGG
  GAGTGCCACCTGTTGGTACTGAAAGCTCTGCAGGATGGCAGGGCATATGGATCTCCATGGTGCAACAAAC
  AAATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAATTGTTAAT
  TCGAAATCATTTGGTTAACATGCAGCAGTATGATCTTCATCTTGCTCAGTCGATGGAGAATGGCTTGAAC
  TATATGGCCGTGGCGTTTGCTATGCAACTGGTAAAAATTCTGCTGGTGGATGAAAGGAGTGTTGCCCATA
  TTACTGAGGCAGATCTGTTCCATACCATTGAAACCCTCATGAGAATTAATGCTCATTCCAGAGGCAATGC
  TCCAGAAGGGTTGCCCCAGCTGATGGAAGTGGTGAGATCCAACTATGAAGCAATGATTGATCGTGCTCAT
  GGAGGCCCTAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAATATGATGACCCTCCAGGCCTGA
  GGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCTGGCCGCGACAG
  TACCAAAGCATTCTCTGCGTTTGTTGGACAGATGCACCAACAAGGAATACTTAAAACTGATGACCTCATA
  ACAAGGTTTTTTCGTCTTTGCACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAGGCGGAGCAGCAGC
  ACAATCCTGCGGCTAACCCTACCATGATCCGGGCCAAGTGCTACCACAACCTGGATGCCTTTGTCCGCCT
  CATTGCACTGTTAGTGAAGCACTCTGGGGAGGCCACCAACACTGTCACAAAGATCAACCTGCTCAATAAG
  GTTCTCGGTATAGTAGTGGGAGTTCTCCTACAGGATCATGATGTTCGGCAGAGTGAATTTCAGCAACTTC
  CGTACCATAGGATTTTTATCATGCTGCTGTTGGAGCTCAATGCACCCGAGCATGTGTTGGAAACCATTAA
  TTTCCAGACTCTTACTGCTTTCTGCAATACATTCCACATCTTGAGACCTACTAAAGCTCCTGGCTTTGTA
  TATGCTTGGCTCGAATTGATTTCCCATCGGATCTTTATTGCAAGAATGCTGGCACATACGCCACAGCAGA
  AGGGGTGGCCTATGTATGCTCAGCTGTTGATTGATTTATTCAAATATTTAGCACCTTTCCTTAGAAATGT
  GGAACTCACTAAACCTATGCAAATCCTCTACAAGGGTACTCTGAGAGTGCTGCTGGTTCTTTTGCATGAT
  TTCCCAGAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCTCCTAATTGTATCCAGTTAA
  GAAATTTGATCCTGAGTGCCTTCCCAAGAAACATGAGGCTCCCAGACCCATTCACTCCTAATCTGAAGGT
  GGACATGTTAAGCGAAATTAACATCGCACCTCGGATCCTCACCAATTTCACTGGAGTGATGCCGCCTCAG
  TTCAAAAAGGATTTGGATTCTTATCTTAAAACTCGGTCGCCGGTCACTTTCCTGTCTGATCTGCGTAGTA
  ACCTACAGGTATCTAATGAGCCCGGGAATCGCTACAACCTCCAGCTTATCAATGCATTGGTGCTCTACGT
  AGGGACTCAGGCTATCGCACACATCCACAACAAGGGCAGCACGCCCTCAATGAGCACCATCACCCACTCA
  GCACACATGGACATCTTCCAGAACCTGGCTGTGGACCTAGACACTGAGGGCCGATATCTCTTCTTAAATG
  CAATTGCGAACCAGCTACGGTACCCAAACAGCCACACTCACTACTTCAGCTGCACAATGTTGTACCTTTT
  TGCTGAGGCGAACACTGAGGCAATCCAGGAACAGATCACAAGGTTATTTCAGTCTGTTGCACAGTGCTGC
  ATGGGACAGAAGCAGGCTCAGCAAGTGATGGAGGGGACCGGTGCCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221036 representing NM_153164
  Red=Cloning site Green=Tags(s)
  
  MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ
  TQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSPD
  LRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTL
  RRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIMQF
  GVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSEGAQAHTWNVEVLIDVLKELNP
  SLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDFIYRPWKHAEGQLSFIQHSLINPEVFC
  FADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQ
  INTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRI
  LDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGG
  LAPEKDQPKSAQLPAETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSA
  SSLDAISPVQIDPLAGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIG
  GLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEID
  DEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITAC
  LFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFP
  HHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVNTMVTTSTTTTVAKTVTVT
  KPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEE
  FMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNL
  GHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSLVFRPPNPWTMAIMNV
  LAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPAITTT
  TTSTTPATSTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELV
  HPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFAS
  ALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVV
  LTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKC
  ITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYR
  ECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLN
  YMAVAFAMQLVKILLVDERSVAHITEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAH
  GGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLI
  TRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNK
  VLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFV
  YAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHD
  FPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQ
  FKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHS
  AHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRLFQSVAQCC
  MGQKQAQQVMEGTGAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153164
• ORF Size: 6978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153164.3, NP_694804.3
• RefSeq Size: 8391
• RefSeq ORF: 6981
• Locus ID: 234594