Polr3a (NM_001081247) Mouse Tagged ORF Clone

CAT#: MG221294

Polr3a (GFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr3a
• Synonyms: 9330175N20Rik; BC053071; RPC1; RPC155
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221294 representing NM_001081247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGGAGCAGTTCCGCGAGACGGATGTGGCTAAGAAAATAAGCCATATCTGTTTTGGAATGAAGT
  CCCCTGAGGAGATGCGCCAGCAGGCACATATCCAGGTTGTCAGTAAGAACCTGTACAGCCAAGACAACAA
  CCACGCCCCCTTGTTGTATGGAGTGCTTGACCATAGAATGGGTACGAGCGAAAAAGATCGTCCTTGTGAG
  ACCTGTGGGAAGAACTTGGCGGACTGTCTCGGCCACTATGGCTACATTGATCTCGAGCTACCGTGCTTTC
  ATGTCGGGTACTTCAGGGCTGTCATTGGTATCTTACAGATGATCTGCAAAACCTGCTGCCACATTATGCT
  GTCCCAGGAAGAGAAGCAGCAGTTTCTAGATTTTCTGAAGAGGCCTGGCCTAACCTACCTGCAGAAGCGA
  GGCCTGAAGAAGAAGATCTCAGATAAATGCCGGAAGAAAAGCACTTGTCACTACTGTGGTGCCTTTAATG
  GTACCGTAAAGAAATGTGGTTTACTGAAGATAATTCATGAAAAATACAAGACAAACAAAAAAGTCGTGGA
  TCCCATTGTATCAAACTTCCTGCAGTCCTTTGAGACAGCCATTGAGCATAACAAAGAGGTTGAACCCCTG
  CTGGGAAGGGCCCAGGAGAACTTGAATCCTTTAGTAGTTCTGAACTTGTTTAAGCGCATCCCAGCTGAAG
  ATGTCCCGTTACTCCTGATGAACCCAGAATCTGGAAAGCCCTCTGATTTGATTCTCACCCGACTTTTGGT
  GCCTCCCCTGTGTATCAGACCGTCCGTCGTGAGTGATCTGAAGTCTGGAACCAATGAAGATGACCTGACA
  ATGAAACTGACAGAGATCATTTTCCTAAATGATGTTATTAAAAAGCACCGGATCTCCGGAGCCAAGACGC
  AGATGATCATGGAGGACTGGGATTTCCTGCAGCTGCAGTGTGCCCTCTACATCAACAGTGAGCTCTCTGG
  CATCCCTCTCAACATGGCACCTAAGAAATGGACGCGAGGCTTTGTGCAGCGCCTGAAGGGGAAACAGGGT
  CGATTTAGAGGTAATCTGTCAGGGAAGAGGGTGGATTTCTCCGGCCGAACAGTCATCTCACCTGACCCCA
  ACCTCCGGATTGATGAAGTAGCCGTGCCAGTTCATGTGGCCAAAATTCTAACTTTTCCTGAGAAGGTGAA
  CAAAGCAAACATCAACTTTTTGAGGAAACTGGTTCGAAATGGCCCAGATGTTCACCCAGGAGCCAATTTT
  ATCCAACAAAGACACATGCAGATGAAGAGGTTCCTGAAGTATGGAAATCGGGAAAAGATGGCTCAGGAGC
  TCAAGTTCGGCGACATTGTAGAGAGACACCTGATAGATGGAGATGTGGTCTTGTTCAACCGGCAGCCCTC
  CCTGCACAAACTGAGCATCATGGCCCACCTGGCCAGGGTTAAGCCCCACCGGACCTTCAGATTCAATGAA
  TGTGTCTGCACCCCCTACAATGCAGACTTTGATGGGGATGAAATGAACCTTCATCTTCCCCAGACAGAGG
  AAGCCAAAGCAGAGGCCCTTGTTCTCATGGGGACCAAAGCGAACCTTGTAACCCCACGAAATGGGGAGCC
  ACTCATTGCTGCCATTCAGGACTTCTTAACAGGTGCTTACCTCCTCACCCTCAAGGACACCTTCTTTGAC
  CGAGCCAAGGCTTGCCAGATCATCGCTTCAATACTGGTTGGCAAAGATGAAAAAATTAAAGTGCGCCTCC
  CACCCCCTACAATACTAAAGCCTGTCACCCTGTGGACCGGAAAGCAGATCTTCAGTGTCATCCTCAGACC
  TAGTGATGACAATCCAGTGCGGGCCAACCTCCGAACCAAGGGCAAGCAGTACTGTGGCAAGGGGGAAGAC
  CTCTGCGTCAACGACTCCTATGTCACCATCCAGAACAGTGAGTTGATGTGCGGCAGCATGGACAAAGGGA
  CCCTGGGGTCGGGGTCCAAGAACAACATCTTCTACATCTTGCTACGAGACTGGGGGCAGAATGAGGCTGC
  CGATGCCATGTCTCGGCTTGCCAGGCTGGCTCCTGTCTACCTGTCTAACCGTGGGTTCTCCATCGGGATT
  GGTGATGTCACGCCTGGCCAGGGACTGCTGAAAGCCAAGTATGAGCTACTAAATGCTGGCTACAAAAAAT
  GTGACGAGTACATTGAAGCCCTGAACACAGGCAAGCTGCAGCAGCAGCCAGGCTGCACGGCCGAGGAGAC
  GCTTGAGGCTCTGATCCTGAAGGAGCTGTCTGTGATCCGGGACCATGCTGGCAGTGCCTGCCTCCGCGAG
  CTGGACAAGAGCAACAGCCCCCTCACCATGGCCCTGTGTGGCTCCAAAGGTTCCTTCATAAACATATCAC
  AGATGATTGCCTGTGTGGGACAGCAAGCCATCAGTGGCTCCCGGGTTCCAGATGGCTTTGAGAACAGATC
  TTTGCCTCACTTTGAGAAGCATTCAAAGCTCCCTGCTGCCAAAGGCTTTGTGGCCAATAGCTTTTACTCT
  GGCTTAACGCCTACTGAGTTTTTCTTCCACACAATGGCCGGTCGGGAGGGTCTGGTGGACACAGCTGTAA
  AGACTGCTGAAACAGGATACATGCAGAGAAGACTGGTCAAATCCCTGGAAGACCTCTGCTCGCAGTACGA
  TCTGACAGTGCGGAGCTCCACCGGTGACATCATCCAGTTCATCTATGGAGGAGATGGCTTAGATCCTGCT
  GCCATGGAGGGCAAAGACGAACCGCTGGAGTTTAAGCGGGTTCTGGACAACATCAAGGCAGTGTATCCAT
  GTCAGACTGAACCTGCTCTCAGTAAGAACGACCTCACCTTGACCGCTGAGGCCATCATGAAGAAGAATGA
  GTTCCTATGCTGCCAAGACAGCTTCCTGCAGGAAATAAAAACATTCATTAAAGGGGTGTCCGAGAAGATT
  AAGAAAACCAGAGATAAATATGGGATCAATGATAACGGCACAACAGAGCCTCGTGTGCTGTACCAGTTGG
  ACCGTATTACCCCTACCCAAATAGAGAAGTTTCTGGAGACCTGTAGGGATAAGTATATGAGAGCACAGAT
  GGAGCCAGGCTCTGCGGTAGGTGCACTCTGTGCACAGAGCATTGGTGAGCCCGGCACCCAGATGACGCTG
  AAGACATTCCACTTTGCAGGAGTAGCCTCCATGAACATCACCCTGGGTGTGCCTCGGATCAAAGAGATCA
  TCAACGCTTCCAAGGCCATCAGCACTCCAATTATCACAGCACAGCTAGACAAGGATGACGACGCGGATTA
  TGCACGCCTGGTGAAGGGCAGGATTGAGAAAACCCTCCTGGGGGAGATCTCAGAGTATATTGAAGAAGTG
  TTTCTTCCTGATGACTGCTTTATTCTTGTCAAGCTCTCCCTGGAACGGATCCGACTTCTGAGACTGGAGG
  TCAATGCTGAGACAGTGCGGTACTCCATCTGCACATCCAAGCTCCGAGTGAAGCCTGGTGATGTGGCCGT
  TCATGGGGAGGCTGTGGTGTGTGTCACCCCCAGGGAAAACAGCAAGAGCTCCATGTATTATGTGCTGCAG
  TTTCTCAAAGAGGATCTGCCCAAGGTGGTTGTACAAGGCATCCCAGAGGTGTCGCGAGCTGTCATCCATA
  TTGACGAGCAGAGTGGGAAGGAGAAGTTCAAGCTTCTGGTGGAAGGTGACAACCTGCGGGCAGTCATGGC
  CACCCACGGTGTGAAAGGCACCCGGACCACGTCTAATAACACCTATGAGGTGGAAAAAACCCTGGGCATT
  GAGGCTGCCCGGACAACTATCATCAACGAGATCCAGTACACGATGGTCAACCACGGCATGAGCATCGACA
  GGAGGCACGTGATGCTGCTCTCTGACCTGATGACCTACAAGGGTGAAGTCCTGGGTATAACCAGGTTTGG
  CCTGGCCAAGATGAAGGAGAGTGTGTTGATGCTGGCCTCCTTTGAAAAGACAGCTGACCATCTCTTTGAT
  GCTGCCTACTTTGGGCAGAAAGACTCTGTATGCGGAGTGTCTGAGTGCATCATCATGGGAATCCCGATGA
  ACATCGGAACTGGGCTCTTCAAGCTGTTACACAAAGCCAACAGGGACCCCAAACCACCCAGGAGACCCCT
  GATATTTGACACACATGAATTCCACATCCCTCTTGTTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221294 representing NM_001081247
  Red=Cloning site Green=Tags(s)
  
  MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNNHAPLLYGVLDHRMGTSEKDRPCE
  TCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKQQFLDFLKRPGLTYLQKR
  GLKKKISDKCRKKSTCHYCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPL
  LGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPESGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLT
  MKLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQG
  RFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVRNGPDVHPGANF
  IQQRHMQMKRFLKYGNREKMAQELKFGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNE
  CVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFD
  RAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGED
  LCVNDSYVTIQNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGI
  GDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRE
  LDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYS
  GLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPA
  AMEGKDEPLEFKRVLDNIKAVYPCQTEPALSKNDLTLTAEAIMKKNEFLCCQDSFLQEIKTFIKGVSEKI
  KKTRDKYGINDNGTTEPRVLYQLDRITPTQIEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTL
  KTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEV
  FLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQ
  FLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKFKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGI
  EAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFD
  AAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKANRDPKPPRRPLIFDTHEFHIPLVT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081247
• ORF Size: 4170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081247.1, NP_001074716.1
• RefSeq Size: 4683
• RefSeq ORF: 4173
• Locus ID: 218832