Kcnj1 (NM_001168354) Mouse Tagged ORF Clone

CAT#: MG221580

Kcnj1 (GFP-tagged) - Mouse potassium inwardly-rectifying channel subfamily J member 1 (Kcnj1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnj1
• Synonyms: Kir1.1; ROMK; Romk2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221580 representing NM_001168354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAAACATCTTCGAAGATGGTTTGTCACTCACATATTTGGGCGTTCTCGGCAACGAGCAAGGTTGG
  TCTCCAAAGATGGAAGGTGTAACATCGAGTTTGGCAATGTAGATGCACAGTCGAGGTTTATATTCTTTGT
  GGATATCTGGACAACTGTACTTGACCTGAAATGGAGGTACAAAATGACCGTGTTCATCACAGCCTTCTTG
  GGGAGTTGGTTTCTCTTTGGTCTCCTGTGGTATGTCGTAGCCTATGTTCATAAGGATCTCCCAGAGTTCT
  ACCCACCTGACAACCGTACTCCTTGTGTGGAGAACATTAATGGCATGACATCAGCCTTTCTGTTTTCTCT
  AGAGACCCAAGTGACCATAGGTTACGGATTCAGGTTTGTGACAGAACAGTGTGCCACTGCCATTTTTCTG
  CTTATCTTCCAGTCTATTCTTGGAGTGATCATCAATTCTTTCATGTGTGGTGCCATATTAGCCAAGATCT
  CTAGACCCAAAAAACGTGCAAAGACCATTACATTCAGCAAGAATGCGGTGATCAGCAAACGTGGGGGGAA
  GCTCTGTCTCCTCATCCGAGTAGCAAATCTTAGGAAAAGCCTTCTGATTGGCAGTCACATATATGGTAAG
  CTTCTGAAGACTACCATCACACCTGAAGGAGAGACCATTATTTTGGATCAGACCAATATAAACTTTGTAG
  TTGATGCTGGCAATGAAAATTTGTTCTTCATTTCCCCACTGACAATCTACCACATTATTGACCACAACAG
  CCCTTTCTTCCACATGGCGGCAGAAACTCTTTCCCAACAGGACTTCGAGTTGGTTGTCTTTTTAGATGGC
  ACAGTAGAATCCACCAGTGCAACCTGCCAAGTCCGCACATCATACATCCCAGAAGAGGTGCTTTGGGGTT
  ACCGTTTTGTTCCCATCGTATCCAAGACCAAGGAAGGGAAATACCGAGTGGATTTCCATAACTTTGGTAA
  GACGGTGGAAGTGGAGACCCCTCATTGTGCCATGTGCCTCTATAATGAGAAAGATGCCAGGGCCAGGATG
  AAGAGAGGCTATGACAACCCTAACTTTGTCTTGTCAGAAGTTGATGAAACAGACGACACCCAAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221580 representing NM_001168354
  Red=Cloning site Green=Tags(s)
  
  MFKHLRRWFVTHIFGRSRQRARLVSKDGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFL
  GSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFL
  LIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGK
  LLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDG
  TVESTSATCQVRTSYIPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARM
  KRGYDNPNFVLSEVDETDDTQM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168354
• ORF Size: 1176 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168354.1, NP_001161826.1
• RefSeq Size: 3075
• RefSeq ORF: 1179
• Locus ID: 56379