Dclre1c (NM_175683) Mouse Tagged ORF Clone

CAT#: MG221669

Dclre1c (GFP-tagged) - Mouse DNA cross-link repair 1C PSO2 homolog (S. cerevisiae) (Dclre1c) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dclre1c
• Synonyms: 9930121L06Rik; AI661365; Art; Snm1l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221669 representing NM_175683
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCTTCCAGGGACAGATGGCGGAGTATCCAACCATCTCCATTGACCGCTTCGACAGGGAGAACC
  TGAAAGCCCGTGCCTACTTCCTTTCGCACTGTCACAAGGATCACATGAAAGGATTAAGGGCTCCTTCCTT
  GAAAAGAAGGCTGGAATGCAGCTTGAAGGTCTTCCTGTACTGTTCTCCAGTCACTAAGGAGCTGTTGTTA
  ACTAGCCCAAAGTACAGATTCTGGGAAAACAGAATTATAACAATTGAAATTGAAACTCCTACGCAGATAT
  CTTTAGTTGATGAGGCTTCGGGTGAGAAGGAAGAGGTTGTTGTGACTCTCTTACCAGCTGGTCACTGCCC
  AGGATCAGTTATGTTTTTATTTCAGGGCAGTAATGGAACTGTCTTATACACAGGAGACTTCAGACTGGCA
  AAAGGAGAAGCTTCCAGAATGGAGCTTCTGCACTCTGGAGGCAGAGTAAAAGACATCCAAAGTGTGTATT
  TAGACACGACTTTCTGTGACCCAAGGTTTTATCAGATCCCAAGTCGTGAGCAGTGTTTGAGGGGCATTTT
  GGAGCTGGTTCGGAGCTGGGTCACTAGGAGTCCGCACCACGTCGTGTGGCTGAACTGTAAAGCAGCTTAT
  GGCTACGAGTATTTATTCACCAACCTAAGCGAGGAGCTGGGAGTTCAGGTTCATGTGGACAAGCTGGACA
  TGTTTAAAAACATGCCTGATATCCTGCACCATCTCACAACGGACAGAAACACCCAGATCCACGCCTGCCG
  CCACCCAAAGGCAGAAGAGTGTTTTCAGTGGAATAAACTACCCTGTGGTATAACTTCCCAAAACAAAACT
  GCACTCCACACAATCAGCATCAAGCCATCTACCATGTGGTTTGGAGAGAGGACCAGAAAAACCAACGTGA
  TCGTTAGGACTGGAGAGAGCTCATACAGAGCTTGCTTCTCTTTTCACTCCTCCTTCAGTGAGATTAAAGA
  TTTTTTGAGCTACATCTGCCCAGTGAATGTGTATCCAAATGTCATCCCAGTTGGCCTCACTGTGGATAAG
  GTCATGGACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAAGTGTTGAGCCAAAGTACAAACCGCTTGGAA
  AATTGAAGAGAGCCAGAACAATCCATCTTGACTCGGAGGAAGACGATGATCTCTTTGATGACCCTCTACC
  GACACCTTTAAGGCACAAAGTTCCGTACCAGCTAACTCTTCAGCCTGAGCTGTTTTCAATGAAGGCACTG
  CCACTAGACCAGCCTGAACTGAGACAAAGCCCAGGAGGCTGCAAAGCAGAGAGTGTGTGGAGCCCTTCTT
  TGGCTAACTTCATAGACTGTGAAGAATCCAACAGCGACAGTGGAGAAGAGCTAGAAACCCCACCACCCTC
  ACTGCAGGGAGGTCTGGGCCCCTCGACACTCGTCCAGCAAAATGCTGATCCGGATGTGGACATACCACAG
  TGGGAAGTCTTCTTCAAAAGAAGAGATGAAATCACAGTGGACACCATGATTAGAACTCCCAGACCCAGAA
  AGATGAAAGGCTGTGGTCAATGGAGTCTGAAGATGCTTCTCCAGAACCTGGAAATACAGGAAGAGAAACA
  TATATTTGAGAACAGGGGCTGGAAGATGGCTGGGCAGGTAAAGGGTAGCTGTGGTCTTTTGGAGGGCCAG
  AGTTCACTCCCAACATTCAAGCTAGCCACCTCCCTAGCCTCAAACTCCAGCTTCTGGCCTCCATGGCACC
  TGCATTCACATGCGCACACACAAATCTTTACCTTTAAAAAAAAAACCAAAACTTTACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221669 representing NM_175683
  Red=Cloning site Green=Tags(s)
  
  MSSFQGQMAEYPTISIDRFDRENLKARAYFLSHCHKDHMKGLRAPSLKRRLECSLKVFLYCSPVTKELLL
  TSPKYRFWENRIITIEIETPTQISLVDEASGEKEEVVVTLLPAGHCPGSVMFLFQGSNGTVLYTGDFRLA
  KGEASRMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREQCLRGILELVRSWVTRSPHHVVWLNCKAAY
  GYEYLFTNLSEELGVQVHVDKLDMFKNMPDILHHLTTDRNTQIHACRHPKAEECFQWNKLPCGITSQNKT
  ALHTISIKPSTMWFGERTRKTNVIVRTGESSYRACFSFHSSFSEIKDFLSYICPVNVYPNVIPVGLTVDK
  VMDVLKPLCRSPQSVEPKYKPLGKLKRARTIHLDSEEDDDLFDDPLPTPLRHKVPYQLTLQPELFSMKAL
  PLDQPELRQSPGGCKAESVWSPSLANFIDCEESNSDSGEELETPPPSLQGGLGPSTLVQQNADPDVDIPQ
  WEVFFKRRDEITVDTMIRTPRPRKMKGCGQWSLKMLLQNLEIQEEKHIFENRGWKMAGQVKGSCGLLEGQ
  SSLPTFKLATSLASNSSFWPPWHLHSHAHTQIFTFKKKTKTLL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175683
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175683.4, NP_783614.1
• RefSeq Size: 4082
• RefSeq ORF: 1812
• Locus ID: 227525
• Gene Summary: This gene encodes a member of the SNM1 family of nucleases and is involved in V(D)J recombination and DNA repair. This protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Homozygous knockout mice for this gene exhibit severe combined immunodeficiency with sensitivity to ionizing radiation. Mutations in this gene in humans can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]