Mthfd1l (NM_172308) Mouse Tagged ORF Clone

CAT#: MG221835

Mthfd1l (GFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mthfd1l
• Synonyms: 2410004L15Rik; AI647056; Fthfsdc1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221835 representing NM_172308
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTCCGCCTCCCTCTCCTGCTTCGCCAGCTCGGCCGCCAGCAGCTGCCCTCGGGCCCGGCGTGTC
  GCCTCCGTGAGCTGTGTCGCTCAGGCAGCAGAAGCAGCAGCAGCGGAGGTGGTGACCCCGAGGGGTTACG
  CGGGCGGCGGCTGCAGGATGGCCAAACCTTCAGCAGCCATGGCCCAGGCAATCCGGAAGCCCCCGGGATG
  GACTCCATCGTCAGAGACGTCATTCACAATTCCAAGGAAGTGCTGAGCTTGTTGCAAGAAAAGAACCCTG
  CCTTTAAGCCAGTGCTTGTGGTTATTCAGGCCGGTGATGACAATTTGATGAAGGATATGAACCAGAATTT
  GGCCAAGGAGGCTGGTCTGGACATCACTCACATCTGCTTGCCTCCAGACAGCGGAGAGGATGAGATCATA
  GATGAAATCCTGAAGATTAATGAAGACCCCAGAGTGCATGGCCTTACCCTTCAGATCTCTGAAGATTCAT
  TGAGCAACAAAGTTCTCAATGCTTTGAAACCAGAAAAAGATGTGGACGGGGTGACCGACATCAATCTGGG
  GAAGCTCGTACGAGGTGACGCTCCGGAGTGTTTTGTTTCACCCTTGGCCAAAGCTGCTGTAGAACTTGTT
  GAAAAGTCAGGGATCACTTTGGATGGAAAGAAGGTCTTGGTCGTAGGGGCGCACGGGCCCCTGGAAGCTG
  CCCTGCAGTGGCTTTTCCAGAGAAAAGGATCTATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCC
  AGACAAGCTTCGAGAGGCAGACATTGTGGTCTTAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGTGTGG
  ATTCCATCAGGAACCACCATTCTCAATTGCTTCCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTC
  CTGGGGTCCCCGTGGACAAACTCATTGCAGAAGAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCA
  GAACATGGTGAGCAGCGGCAGGAGGTGGCTCAGAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTG
  AAACTGCAGCCTCTCTCACCTGTGCCCAGTGATATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGG
  ATGTCCTTGCCAAGGAGATAGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGT
  CCAATTGTCCCTGCTGGAGAGGTTAAAGGATCAAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACG
  CCCACCCCGCTCGGAGAAGGGAAGAGTACAGTCACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGA
  AAGTCAACTCCTTCGCCTGTCTGAGGCAGCCTTCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGC
  AGGAGGTGGATATGCTCAGGTCATCCCCATGGAGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCC
  ATCACTGCCGCTAATAACTTGCTGGCCGCAGCCATCGACACGAGAATTTTACATGAGAGCACTCAAACAG
  ACAAGGCTCTCTACAATCGACTGGTTCCCTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTC
  TCGGCTGAAGAAACTGGGGATACATAAGACTGATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTC
  GCCCGCCTCAACATTGACCCCGCAACCATCACGTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGA
  GAAAAATAACCATTGGACAGGGAAGTACAGAGAAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGT
  GGCCAGCGAGATCATGGCCGTGCTGGCCTTGACTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGA
  ATGGTGGTGGCCAGTGACAAAGACGGGCAGCCTGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGA
  CAGTTTTGATGAAAGACGCCATCAAGCCAAATCTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCA
  CGCTGGTCCCTTTGCCAACATCGCTCACGGCAACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTG
  GTCGGCGAAGAAGGGTTTGTGGTGACTGAAGCTGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCA
  ATATCAAATGCCGAGCTTCTGGCCTGGTGCCCAACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAA
  GATGCATGGAGGCGGGCCGAGTGTAACTGCTGGGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATT
  CAGCTGGTAGCTGATGGCTGCTGCAACCTACAGAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGG
  TGGTGGTGGCACTGAATGTCTTCAAGACGGACACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAA
  ACGGGCCGGTGCCTTTGATGCGGTCCCCTGCTATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTG
  GCCCGGGCTGTGAGAGAAGCTGCGAATAAAAGAAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCA
  TCGTGGAAAAGATAAGAGTCATTGCCCAGACTGTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGC
  GCAGTCCAAAATCGATCGCTATACGCAACAGGGTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCAC
  CTCTCTCTCTCTCATGAACCTGACAAAAAGGGAGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCC
  GGGCCAGCATCGGGGCTGGGTTCATTTATCCTTTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCAC
  CCGACCCTGCTTTTATGATATAGACCTTGACACAGAAACGGAGCAAGTTAAAGGCTTGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221835 representing NM_172308
  Red=Cloning site Green=Tags(s)
  
  MSVRLPLLLRQLGRQQLPSGPACRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGM
  DSIVRDVIHNSKEVLSLLQEKNPAFKPVLVVIQAGDDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEII
  DEILKINEDPRVHGLTLQISEDSLSNKVLNALKPEKDVDGVTDINLGKLVRGDAPECFVSPLAKAAVELV
  EKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAVW
  IPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCL
  KLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGIT
  PTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHA
  ITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKF
  ARLNIDPATITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGR
  MVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKL
  VGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENI
  QLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDL
  ARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQTVYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTH
  LSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172308
• ORF Size: 2931 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172308.4, NP_758512.3
• RefSeq Size: 3731
• RefSeq ORF: 2934
• Locus ID: 270685