Erbin (NM_001005868) Mouse Tagged ORF Clone

CAT#: MG221851

Erbb2ip (GFP-tagged) - Mouse Erbb2 interacting protein (Erbb2ip) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Erbin
• Synonyms: 1700028E05Rik; Erbb2ip; mKIAA1225
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221851 representing NM_001005868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACAAAACGAAGTTTGTTTGTGCGGTTGGTACCATGTCGCTGTCTACGAGGAGAAGAGGAGACAG
  TCACTACTCTAGATTATTCTCATTGCAGCTTGGAACAAGTTCCCAAAGAGATTTTCACCTTTGAAAAAAC
  TTTAGAGGAGCTCTATTTAGATGCTAATCAGATTGAAGAGCTTCCAAAGCAACTTTTTAATTGTCAGTCT
  CTACATAAACTAAGTTTGCCAGACAATGATTTAACAACATTACCAGCCTCCATTGCAAATCTTATTAATC
  TCAGGGAACTGGATGTCAGCAAAAATGGAATACAAGAATTTCCAGAAAATATTAAAAATTGTAAAGTTTT
  GACAATTGTGGAGGCTAGTGTGAACCCTATTTCTAAGCTCCCTGATGGGTTTTCTCAGCTGCTAAACCTA
  ACCCAGTTATATCTGAATGATGCTTTCCTTGAATTCTTACCAGCTAATTTTGGCAGATTAACTAAACTCC
  AGATTCTAGAACTTAGAGAAAACCAGTTGAAAATGTTGCCTAAAACCATGAATAGACTGACCCAGCTGGA
  AAGGCTGGATTTGGGAAGTAATGAATTCACAGAAGTGCCTGAAGTACTTGAACAACTAAGTGGATTGAGA
  GAGTTTTGGATGGATGGCAATAGACTGACTTTTATTCCAGGGTTTATTGGTAGTTTGAGACAGCTCACGT
  ACTTGGATGTTTCTAAAAATAATATTGAAATGGTTGAAGAAGGAATTTCAACATGTGAAAACCTCCAGGA
  CTTTCTCTTATCAAGTAATTCCCTTCAGCAGCTGCCGGAAACTATTGGTTCATTGAAAAATGTCACAACT
  CTAAAAATTGATGAAAACCAATTAATGTATCTACCAGACTCAATAGGAGGGTTAAGGTCAATAGAAGAAC
  TGGATTGCAGTTTCAATGAAATTGAAGCTTTGCCTTCCTCAATTGGCCAGCTCACAAACATGAGAACATT
  TGCTGCAGATCACAATTACTTACAACAGCTACCCCCAGAGATTGGAAACTGGAAAAATATAACTGTGCTG
  TTTCTCCATTGTAATAAACTAGAGACGCTTCCAGAGGAAATGGGTGATATGCAAAAATTAAAAGTCATCA
  ATTTAAGTGATAATAGGTTAAAGAATTTGCCTTTTAGCTTTACAAAGCTACAACAGTTGACTGCTATGTG
  GCTGTCAGATAATCAGTCCAAACCTCTGATACCTCTTCAAAAAGAAACAGACACAGAGACTCAGAAAATG
  GTGCTTACTAATTACATGTTCCCTCAGCAGCCACGGACTGAAGACGTTATGTTCATATCAGATAATGAAA
  GTTTTAATCCTGCATTGTGGGAAGAACAGAGAAAACAGCGGGCTCAAGTGGCATTTGAATGTGATGAAGA
  CAAAGATGAAAGAGAGGCACCACCCAGGGAGGGAAACTTAAAGAGATATCCAACACCATACCCAGATGAG
  CTTAAGAATATGGTCAAGACTGTTCAAACCATTGTACATAGATTAAAAGATGAAGAGACTAATGAAGAGT
  CTGGGAGAGATTTGAAGCAGCATGAAGATCAGCAAGTTGTAAATAAGGATAAGTGTGTGAAGACTTCAGA
  AAGTACTACAACAAAAAGCAAACTTGATGAAAGAGAAAAGTATATGAATTCTGTACAGAAAATGAGTGAG
  CCTGAAGCTGAGACCAATGGTGGAAATTTGCCAGTAACTGCAAGTATGAAGTTGTCTGGAAACCTGAAGC
  ATATTGTTAACCATGATGATGTTTTTGAGGAATCTGAAGAACTTTCTTCTGATGAAGAAATGAAAATGGC
  AGAGATGCGACCTCCATTAATTGAATCCTCGATTAATCAGCCCAAAGTTGTAGCACTTAGTAATAATAAA
  AAGGATGATGCGAAGGATGCAGACTCTTTATCCGATGAAGTCACACACAACAGTAATCAGAATAACAGCA
  ACTGTTCTTCTCCGTCCCGAATGTCTGATTCAGTGTCTCTTAATACTGACAGCAGTCAAGACACGTCACT
  CTGTTCTCCCGTGAAACAGACTCCCGTGGATAGTAACTCCAAAGTCAGACAAGAAGATGAAAATTTTAAC
  AGCCTTTTACAAAATGGAGTTAATTTAAACAATTCACCAGAGGAAAAATTCAAAATTAATGATAAAAAGG
  ATTTTAAGTTACCTGAGTATGATTTGAATATTGAAGAACAGTTAGTTCTGATTGAAAAAGACATTGATTC
  AAAAGCCACATCTGATGACTCTCGCCAGTTAGACCATATCAACATGAATATTAATAAACTTGTGACCAAT
  AATATTTTTCAACCAGAAGTAATGGAAAGATCAAAGATGCAGGATATAGTACTTGGAACAGGCTTTTTAA
  GTATTCATCCTAAAAATGAGGCTGAGCACATAGAAAATGGGGCTAAGTTTCCGAATTTGGAGTCCATAAA
  TAAGGTAAATGGTCTTTGTGAGGACACTGCACCGTCTCCTGGTAGGGTTGAACCACAGAAGGCCAGTTCT
  TCTGCTGACGTGGGCATTTCTAAAAGCACGGAAGATCTATCTCCTCAGAGAAGTGGTCCAACTGGAGCTG
  TTGTGAAATCTCATAGTATAACTAACATGGAGACTGGAGGTTTAAAAATCTATGACATTCTTGGTGATGA
  TGGCCCTCAGCCGCCAAGTGCAGCAGTTAAAATCGCATCTGCTGTGGATGGGAAGAACATAGTCAGAAGC
  AAGTCTGCCACACTGCTGTATGATCAGCCCCTGCAGGTGTTCACTGCTGCCTCCTCATCTTCTGAGTTAC
  TGTCAGGTACAAAGGCAGTTTTCAAGTTTGATTCAAATCACAACCCTGAAGAGCCAGATATAATAAGAGC
  TGCCACAGTATCTGGCCCACAGTCTACACCTCACCTGTATGGTCCCCCACAATATAATGTCCAGTACAGT
  GGCAGTGCTACAGTGAAAGACACCTTGTGGCACCCTAAACAGAATCCACAAATAGATCCTGTCAGTTTTC
  CTCCTCAGCGCCTTCCAAGATCTGAGAGTGCAGAAAATCACAGTTATGCAAAGCATTCTGCCAACATGAA
  TTTCTCTAACCATAACAATGTTAGAGCCAATACTGGATACCACTTACAACAGAGACTTGCCCCAGCCAGG
  CATGGAGAAATGTGGGCTATCTCCCCGAATGACCGACTAGTGCCAGCAGTCACTCGGACTACTATTCAGA
  GACAGAGTAGCGTGTCTTCAACGGCTTCTGTAAATCTCGGTGACCCCACAAGGCGTACTGAAGGAGATTA
  CTTATCGTACAGAGAGTTACATTCAATGGGAAGAACTCCAGTCATGTCAGGATCACAGAGACCTCTTTCT
  GCACGAGCGTACAGCATCGATGGCCCAAATACATCCAGGCCTCAGAGTGCCCGTCCCTCTATTAATGAAA
  TACCAGAGAGAACTATGTCAGTTAGTGATTTCAATTACTCACGGACTAGTCCTTCAAAAAGACCAAATAC
  AAGGGTCGGGTCTGAACATTCTCTGTTAGATCCTCCAGGAAAAAGCAAGGTTCCTCATGACTGGCGGGAA
  CAAGTACTACGACACATTGAGGCCAAAAAGTTAGAAAAGAGTATGCTGTCAAGGTCCTTTAATTCCAATC
  TTACTGCTGTAAGCAGTTCTCACTATGGCAGCTCTAGGGATCTGCATGGCAGCCAGGGCAGCCTTGCCCT
  GAGTGTTGCAGACGGAAGAGGTTCTGGTGGGCACATTTTTCGACATCCGCAGACATCCAGTCCAGGAGAG
  TGTTGCCAAGATGATAGATTCATGTCAGAAGAGCAGAACCACCCATCAGGTGCACTTAGTCACAGAGGTC
  TTCCAGACAGCCTGATGAAAATGCCTTTGAGTAATGGACAAATGGGCCAGCCTCTCAGGCCTCAGGCACA
  TTATAGTCAAACCCATCACCCCCCTCAGGCATCTGTGGCAAGGCATCCCTCTAGAGAACAACTAATTGAT
  TATTTGATGCTGAAAGTGGCCCACCAGCCTCCATATACACATCCCCATTGTTCTCCTAGACAAGGCCATG
  AACTGGCAAAGCAAGAGATTCGAGTAAGAGTAGAAAAGGATCCAGAACTTGGATTTAGCATATCAGGAGG
  TGTCGGCGGCAGAGGAAACCCTTTCAGACCTGATGATGATGGTATATTTGTAACAAGGGTACAACCTGAA
  GGACCAGCATCAAAATTACTACAGCCAGGAGATAAAATTATTCAGGCTAATGGCTACAGTTTTATCAACA
  TTGAACATGGGCAAGCGGTGTCCTTGTTAAAAACTTTCCATAATGCAGTAGATCTCATCATTGTACGAGA
  AGTTTCTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221851 representing NM_001005868
  Red=Cloning site Green=Tags(s)
  
  MTTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEKTLEELYLDANQIEELPKQLFNCQS
  LHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQEFPENIKNCKVLTIVEASVNPISKLPDGFSQLLNL
  TQLYLNDAFLEFLPANFGRLTKLQILELRENQLKMLPKTMNRLTQLERLDLGSNEFTEVPEVLEQLSGLR
  EFWMDGNRLTFIPGFIGSLRQLTYLDVSKNNIEMVEEGISTCENLQDFLLSSNSLQQLPETIGSLKNVTT
  LKIDENQLMYLPDSIGGLRSIEELDCSFNEIEALPSSIGQLTNMRTFAADHNYLQQLPPEIGNWKNITVL
  FLHCNKLETLPEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSDNQSKPLIPLQKETDTETQKM
  VLTNYMFPQQPRTEDVMFISDNESFNPALWEEQRKQRAQVAFECDEDKDEREAPPREGNLKRYPTPYPDE
  LKNMVKTVQTIVHRLKDEETNEESGRDLKQHEDQQVVNKDKCVKTSESTTTKSKLDEREKYMNSVQKMSE
  PEAETNGGNLPVTASMKLSGNLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIESSINQPKVVALSNNK
  KDDAKDADSLSDEVTHNSNQNNSNCSSPSRMSDSVSLNTDSSQDTSLCSPVKQTPVDSNSKVRQEDENFN
  SLLQNGVNLNNSPEEKFKINDKKDFKLPEYDLNIEEQLVLIEKDIDSKATSDDSRQLDHINMNINKLVTN
  NIFQPEVMERSKMQDIVLGTGFLSIHPKNEAEHIENGAKFPNLESINKVNGLCEDTAPSPGRVEPQKASS
  SADVGISKSTEDLSPQRSGPTGAVVKSHSITNMETGGLKIYDILGDDGPQPPSAAVKIASAVDGKNIVRS
  KSATLLYDQPLQVFTAASSSSELLSGTKAVFKFDSNHNPEEPDIIRAATVSGPQSTPHLYGPPQYNVQYS
  GSATVKDTLWHPKQNPQIDPVSFPPQRLPRSESAENHSYAKHSANMNFSNHNNVRANTGYHLQQRLAPAR
  HGEMWAISPNDRLVPAVTRTTIQRQSSVSSTASVNLGDPTRRTEGDYLSYRELHSMGRTPVMSGSQRPLS
  ARAYSIDGPNTSRPQSARPSINEIPERTMSVSDFNYSRTSPSKRPNTRVGSEHSLLDPPGKSKVPHDWRE
  QVLRHIEAKKLEKSMLSRSFNSNLTAVSSSHYGSSRDLHGSQGSLALSVADGRGSGGHIFRHPQTSSPGE
  CCQDDRFMSEEQNHPSGALSHRGLPDSLMKMPLSNGQMGQPLRPQAHYSQTHHPPQASVARHPSREQLID
  YLMLKVAHQPPYTHPHCSPRQGHELAKQEIRVRVEKDPELGFSISGGVGGRGNPFRPDDDGIFVTRVQPE
  GPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKTFHNAVDLIIVREVSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005868
• ORF Size: 4350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005868.1, NP_001005868.1
• RefSeq Size: 6602 bp
• RefSeq ORF: 4353 bp
• Locus ID: 59079
• Cytogenetics: 13 D1