Pfkm (NM_001163488) Mouse Tagged ORF Clone

CAT#: MG221898

Pfkm (GFP-tagged) - Mouse phosphofructokinase muscle (Pfkm) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pfkm
• Synonyms: AI131669; ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka; Pfkx
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221898 representing NM_001163488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCATGAAGAGCATCATGCAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG
  GTGGAGATGCCCAAGGTATGAATGCTGCCGTCAGGGCTGTGGTCCGAGTTGGTATCTTCACGGGCGCCCG
  CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGACGGTGGCGAGCACATCAGGGAGGCCACGTGG
  GAGAGCGTGTCTATGATGCTTCAGCTGGGTGGCACAGTGATTGGAAGTGCCCGATGCAAGGACTTCCGGG
  AGCGAGAAGGACGACTCCGGGCCGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG
  AGGCGATGGCAGCCTCACTGGGGCTGACACTTTCCGTTCAGAGTGGAGCGACTTGCTGAATGATTTCCAG
  AAAGACGGGAAGATCACAGCCGAGGAGGCTACAAAGTCCAGCTACCTGAACATCGTGGGCCTGGTTGGCT
  CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACTGACTCTGCCCTGCACAGGATTGTGGA
  GATCGTAGACGCCATCACCACCACGGCTCAGAGCCACCAGAGGACGTTTGTGTTAGAAGTGATGGGCCGC
  CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCCTGTGGGGCCGACTGGGTTTTTATTCCTGAGTGTC
  CGCCAGACGATGACTGGGAAGAACACCTTTGTCGCCGGCTCAGTGAGACAAGGACCCGTGGCTCTCGTCT
  CAACATCATCATTGTTGCTGAAGGTGCCATCGACAAGAACGGGAAGCCAATCACCTCAGAAGACATCAAG
  AACCTGGTGGTGAAGCGCCTTGGATATGACACCCGGGTCACTGTTCTGGGACATGTACAGCGGGGTGGGA
  CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG
  GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAACCAGGCTGTGCGTTTGCCCCTCATGGAG
  TGCGTGCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA
  GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA
  GGGTGGGTTGCACACAGTGGCCGTGATGAATGTGGGGGCCCCAGCTGCTGGCATGAATGCCGCTGTTCGC
  TCTACCGTGAGGATTGGCCTTATCCAGGGCAACCGGGTGCTGGTCGTACATGATGGCTTTGAGGGTCTGG
  CCAAAGGTCAGATTGAGGAGGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT
  TGGTACTAAAAGGACTCTCCCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTTTAACATC
  CAGGGCCTTGTCATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAAGGCAGGAAGC
  AGTTTGATGAGCTCTGCATCCCGTTTGTGGTCATTCCGGCCACGGTTTCCAATAACGTCCCTGGGTCAGA
  CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACTTGTGACCGAATCAAGCAGTCTGCA
  GCAGGCACCAAACGTCGGGTGTTTATCATCGAGACTATGGGTGGCTACTGTGGCTACCTGGCCACCATGG
  CAGGGCTGGCCGCTGGGGCTGATGCTGCCTACATTTTTGAGGAACCCTTCACCATTCGCGATCTCCAGGT
  GAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAA
  TGCAATGAGAACTACACTACTGACTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTTGACA
  GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAGCCCAACTCCCTTTGACAGGAATTTTGCCAC
  TAAGATGGGTGCTAAGGCTATGAACTGGATGTCTGGGAAAATCAAGGAGAGTTACCGTAATGGACGGATC
  TTTGCCAACACCCCTGACTCAGGCTGCGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTAA
  CTGAGCTGAAGGACCAGACAGACTTTGAACACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC
  AATCCTCAAAATCCTAGCCAAGTACGAGATTGATCTGGACACCTCCGACCACGCCCACCTGGAGCACATT
  TCAAGGAAACGGTCTGGAGAAGCCGCCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221898 representing NM_001163488
  Red=Cloning site Green=Tags(s)
  
  MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATW
  ESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDFQ
  KDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGR
  HCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIK
  NLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLME
  CVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVR
  STVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNI
  QGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSA
  AGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEK
  CNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRI
  FANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHI
  SRKRSGEAAV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163488
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001163488.1, NP_001156960.1
• RefSeq Size: 2830
• RefSeq ORF: 2343
• Locus ID: 18642