Azin1 (NM_018745) Mouse Tagged ORF Clone

CAT#: MG221901

Azin1 (GFP-tagged) - Mouse antizyme inhibitor 1 (Azin1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Azin1
• Synonyms: 1700085L02Rik; AZI; Oazi; Oazin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221901 representing NM_018745
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGGATTTATTGACGATGCGAACTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATG
  TTATTGATAACTATGTTTATGAACATACCCTGACAGAGAAAAATGCATTTTTTGTGGGGGATCTTGGGAA
  GATCGTGAAGAAGCACAGTCAGTGGCAGACCGTGGTGGCTCAGATAAAGCCGTTTTACACGGTGAAGTGC
  AACTCCACTCCAGCCGTGCTTGAGATCTTGGCAGCTCTTGGAACTGGGTTTGCTTGTTCCAGCAAAAATG
  AAATGGCTTTAGTGCAAGAATTGGGTGTATCTCCAGAAAACATCATTTTCACAAGTCCTTGTAAGCAAGT
  GTCTCAGATAAAGTATGCAGCAAAAGTTGGAGTAAATATTATGACATGTGACAATGAGATTGAATTAAAG
  AAAATTGCAAGGAATCACCCAAATGCCAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTG
  AAGATGGTAACATGAAGTTTGGCACTACACTGAAGAATTGTAGGCATCTTTTGGAATGTGCCAAGGAACT
  TGATGTCCAAATAATTGGGGTTAAATTTCATGTTTCAAGTGCTTGCAAAGAATATCAAGTATATGTACAT
  GCCCTGTCTGATGCTCGATGTGTGTTTGACATGGCTGGAGAGTTTGGCTTTACAATGAACATGTTAGACA
  TCGGTGGAGGCTTCACAGGAACTGAAATTCAGTTGGAAGAGGTTAATCATGTTATCAGTCCTCTGTTGGA
  TATTTACTTCCCTGAAGGATCTGGCATTCAGATAATTTCAGAACCTGGAAGCTACTATGTATCTTCTGCG
  TTTACACTTGCAGTCAATATTATTGCTAAGAAAGTTGTTGAAAATGATAAATTTTCCTCTGGAGTAGAAA
  AAAATGGGAGTGATGAGCCAGCCTTCGTGTATTACATGAATGATGGTGTTTATGGTTCTTTTGCGAGTAA
  GCTTTCTGAGGACTTAAATACCATTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTTTACA
  AGCAGCCTTTGGGGTCCATCCTGTGATGAGCTTGATCAAATTGTGGAAAGCTGTCTTCTTCCTGAGCTGA
  ATGTGGGAGATTGGCTTATCTTTGATAACATGGGAGCAGATTCTTTCCACGAACCATCTGCTTTTAATGA
  TTTTCAGAGGCCAGCTATTTATTTCATGATGTCATTCAGTGATTGGTATGAGATGCAAGATGCTGGAATT
  ACTTCAGATGCAATGATGAAAAACTTCTTCTTTGCACCCTCTTGTATTCAGCTGAGCCAAGAAGACAGCT
  TTTCCACTGAAGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221901 representing NM_018745
  Red=Cloning site Green=Tags(s)
  
  MKGFIDDANYSVGLLDEGTNLGNVIDNYVYEHTLTEKNAFFVGDLGKIVKKHSQWQTVVAQIKPFYTVKC
  NSTPAVLEILAALGTGFACSSKNEMALVQELGVSPENIIFTSPCKQVSQIKYAAKVGVNIMTCDNEIELK
  KIARNHPNAKVLLHIATEDNIGGEDGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKEYQVYVH
  ALSDARCVFDMAGEFGFTMNMLDIGGGFTGTEIQLEEVNHVISPLLDIYFPEGSGIQIISEPGSYYVSSA
  FTLAVNIIAKKVVENDKFSSGVEKNGSDEPAFVYYMNDGVYGSFASKLSEDLNTIPEVHKKYKEDEPLFT
  SSLWGPSCDELDQIVESCLLPELNVGDWLIFDNMGADSFHEPSAFNDFQRPAIYFMMSFSDWYEMQDAGI
  TSDAMMKNFFFAPSCIQLSQEDSFSTEA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018745
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018745.5, NP_061215.1
• RefSeq Size: 4868
• RefSeq ORF: 1347
• Locus ID: 54375
• Gene Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]