Itga2b (NM_010575) Mouse Tagged ORF Clone

CAT#: MG222526

Itga2b (GFP-tagged) - Mouse integrin alpha 2b (Itga2b), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itga2b
• Synonyms: AI172977; alphaIIb; CD41; CD41B; GpIIb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222526 representing NM_010575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGCCTCCTGCGCATGGCATTCCCTGTGGCTTCTGCAGTGGACACCACTGTTCTTGGGTCCTA
  GTGCTGTTCCTCCAGTCTGGGCCTTGAACCTGGACTCTGAGAAGTTCTCCGTCTATGCAGGTCCCAATGG
  CAGCCACTTTGGCTTCTCAGTGGACTTTCATAAGGACAAACATGGAAGCGTGTCCATCGTGGTGGGCGCC
  CCCAGGGCCCTAAACGCAAGTCAAGAGGAGACTGGAGCCGTATTCCTGTGCCCCTGGAAGGCCAATGGTG
  GCAAGTGCAACCCGTTGCTCTTCGACCTCAGGGATGAGACACGAAACCTAGGCTTCCAAATTTTCCAAAC
  CTTCAAGACCGGGCAAGGACTTGGGGCGTCGGTCGTCAGCTGGAATGACGTCATTGTGGCCTGTGCCCCC
  TGGCAGCACTGGAATGTCCTGGAAAAGCGCGATGAGGCAGAGAAGACTCCGGTAGGAGGCTGCTTCTTGG
  CTCAGCTCCAAAGCGGGGGCCGCGCAGAGTACTCGCCCTGTCGCGCCAACACCATGAGCTCCGTTTACGC
  AGAGAGTTTTCGCGGAGACAAGCGATACTGTGAAGCAGGCTTCAGCTTGGCGGTGACCCAGGCTGGGGAG
  CTAGTGCTTGGAGCCCCTGGAGGCTACTTTTTTTTAGGTCTCCTGGCTCGCGTTCCAATTGAGAACATCA
  TCTCCAGCTACCGCCCGGGTACCCTTTTGTGGCATGTTTCCAACCAGCGCTTCACCTACGACAACAGCAA
  CCCAGTGTTTTTCGATGGTTACCGGGGATATTCGGTAGCCGTGGGCGAGTTTGATGGGGATCCGAGCACT
  ACAGAGTACGTATCGGGTGCCCCCACTTGGAGCTGGACCTTGGGAGCGGTGGAAATTTTGGACTCCTACT
  ACCAGCCCCTGCACCGGCTTCATGGAGAACAGATGGCTTCATATTTCGGGCACTCGGTGGCAGTCACTGA
  CGTCAACGGGGACGGGAGGCATGACCTACTGGTGGGGGCTCCATTGTATATGGAGAGCAGGGCAGACCGC
  AAGCTGGCCGAGGTGGGCCGCGTTTATTTGTTTCTGCAGCCTAAGGGCCCCCAAGCTCTGAGCACACCCA
  CTCTCCTGCTGACTGGCACCCAGCTCTATGGGAGATTTGGATCTGCCATTGCACCCCTGGGTGACCTCAA
  CCGAGACGGCTATAATGATATTGCTGTGGCTGCCCCCTATGGGGGTCCCAGTGGTCAGGGCCAAGTGCTG
  ATATTCCTGGGTCAGAGTGAAGGGCTGAGTCCACGCCCCTCCCAGGTTCTGGACAGCCCCTTCCCCACAG
  GCTCTGGCTTTGGCTTCTCCCTTCGTGGTGCTGTAGACATCGATGACAATGGATACCCAGACCTGATTGT
  GGGAGCATATGGAGCTAGCAAGGTGGCTGTGTACAGAGCTCAGCCTGTGGTGATGGCCACTGTCCAACTG
  ATGGTTCAAGACTCCCTGAATCCAACACTGAAGAACTGTGTCCTGGATCAGACCAAGACACCAGTCAGCT
  GCTTCAACATCCAGATGTGTGTGGGAGCCACAGGACACAACATTCCTCAGAAGCTGCATCTAAAGGCAGA
  GCTGCAGCTGGACTTACAGAAGCCCCGTCAGGGCCGCCGGGTGCTCCTGCTGGCATCCCAACAGGCGAGC
  CTCACCCTGAGCCTGGACCTGGGCGGAAGAGACAAGCCTATCTGCCACACCACGGGGGCCTTCCTTCGGG
  ATGAGGCCGACTTCCGGGACAAGCTGAGCCCAATTGTGCTAAGCCTCAACGTGTCGCTGCCCCCAGAAGA
  GACTGGAGGAGCCCCTGCCGTGGTATTGCATGGAGAAACCCATGTCCAGGAGCAGACACGGATCATCCTG
  GATTGTGGGGAAGACGACCTGTGTGTGCCACAGCTCCGGCTCACAGCTACTGCGGGGGACTCCCCACTCC
  TAATCGGTGCTGACAATGTGTTGGAGCTGAAGATTGAAGCAGCCAATGATGGTGAGGGAGCCTATGAAGC
  GGAGCTGGCTGTGCATCTGCCTCCAGGTGCCCACTACATGCGGGCTCTCAGCAACATTGAGGGCTTTGAG
  AGGCTTGTCTGCACTCAGAAGAAAGAGAATGAGTCCAGGGTGGCCCTCTGTGAGCTGGGCAACCCCATGA
  AAAAGGACACCCGGATAGGAATCACAATGCTGGTGAGTGTGGAGAACCTGGAAGAAGCTGGCGAGTCCGT
  GTCCTTCCAGCTGCAGGTCAGGAGCAAGAACAGCCAGAATCCAAACAGTAAGGTCGTGATGTTGCCTGTG
  GCAATCCAAGCTGAAGCCACAGTGGAGCTTCGAGGGAATTCCTTCCCTGCCTCCCTGGTGGTGGCAGCAG
  AAGAAGGTGACAGGGAGCAGGAAGACTTGGACAGATGGGTCTCCAGGCTGGAGCACACCTATGAGCTCCA
  CAACATTGGCCCTGGCACTGTGAATGGCCTCAGACTTCTCATCCACATTCCTGGCCAGTCCCAGCCCTCG
  GATCTGCTCTACATCCTGGATGTGCAGCCGCAGGGAGGTCTCCTTTGCTCCACACAGCCATCTCCCAAGG
  TGGACTGGAAACTATCCACGCCCAGCCCTTCTTCCATTCGCCCCGTCCATCACCAGCGTGAGCGCAGACA
  GGCATTCCTGCAGGGGCCCAAGCCAGGGCAGCAGGACCCAGTTCTGGTGAGCTGCGACGGCTCAGCGTCC
  TGTACGGTGGTGGAGTGTGAGCTGCGGGAGATGGTGCGCGGGCAGCGGGCCATGGTTACTGTGCAGGTCA
  TGCTGGGGCTGTCCAGCCTCCGCCAGAGGCCGCAGGAGCAGTTTGTGCTGCAGTCGCACGCCTGGTTCAA
  CGTCTCCTCCCTACCTTACTCGGTGCCGGTGGTCAGCTTGCCCAGTGGGCAAGCTCGGGTGCAGACACAG
  CTGCTTCGGGCCTTGGAGGAGAGGGCCATTCCTGTCTGGTGGGTGCTGGTGGGCGTGCTGGGCGGTCTGC
  TGCTGCTGACCCTGCTAGTTTTGGCCATGTGGAAGGCTGGCTTCTTCAAGCGGAATCGACCGCCTCTGGA
  GGAAGATGAAGAGGAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222526 representing NM_010575
  Red=Cloning site Green=Tags(s)
  
  MARASCAWHSLWLLQWTPLFLGPSAVPPVWALNLDSEKFSVYAGPNGSHFGFSVDFHKDKHGSVSIVVGA
  PRALNASQEETGAVFLCPWKANGGKCNPLLFDLRDETRNLGFQIFQTFKTGQGLGASVVSWNDVIVACAP
  WQHWNVLEKRDEAEKTPVGGCFLAQLQSGGRAEYSPCRANTMSSVYAESFRGDKRYCEAGFSLAVTQAGE
  LVLGAPGGYFFLGLLARVPIENIISSYRPGTLLWHVSNQRFTYDNSNPVFFDGYRGYSVAVGEFDGDPST
  TEYVSGAPTWSWTLGAVEILDSYYQPLHRLHGEQMASYFGHSVAVTDVNGDGRHDLLVGAPLYMESRADR
  KLAEVGRVYLFLQPKGPQALSTPTLLLTGTQLYGRFGSAIAPLGDLNRDGYNDIAVAAPYGGPSGQGQVL
  IFLGQSEGLSPRPSQVLDSPFPTGSGFGFSLRGAVDIDDNGYPDLIVGAYGASKVAVYRAQPVVMATVQL
  MVQDSLNPTLKNCVLDQTKTPVSCFNIQMCVGATGHNIPQKLHLKAELQLDLQKPRQGRRVLLLASQQAS
  LTLSLDLGGRDKPICHTTGAFLRDEADFRDKLSPIVLSLNVSLPPEETGGAPAVVLHGETHVQEQTRIIL
  DCGEDDLCVPQLRLTATAGDSPLLIGADNVLELKIEAANDGEGAYEAELAVHLPPGAHYMRALSNIEGFE
  RLVCTQKKENESRVALCELGNPMKKDTRIGITMLVSVENLEEAGESVSFQLQVRSKNSQNPNSKVVMLPV
  AIQAEATVELRGNSFPASLVVAAEEGDREQEDLDRWVSRLEHTYELHNIGPGTVNGLRLLIHIPGQSQPS
  DLLYILDVQPQGGLLCSTQPSPKVDWKLSTPSPSSIRPVHHQRERRQAFLQGPKPGQQDPVLVSCDGSAS
  CTVVECELREMVRGQRAMVTVQVMLGLSSLRQRPQEQFVLQSHAWFNVSSLPYSVPVVSLPSGQARVQTQ
  LLRALEERAIPVWWVLVGVLGGLLLLTLLVLAMWKAGFFKRNRPPLEEDEEEE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010575
• ORF Size: 3099 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010575.2, NP_034705.2
• RefSeq Size: 3437
• RefSeq ORF: 3102
• Locus ID: 16399
• Gene Summary: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProtKB/Swiss-Prot Function]