Ltk (NM_206942) Mouse Tagged ORF Clone

CAT#: MG222630

Ltk (GFP-tagged) - Mouse leukocyte tyrosine kinase (Ltk) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ltk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222630 representing NM_206942
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTCCCACCGGCTGCTGCTGTGGCTGGGAGCTGCTGGAACTATTCTTTGCTCCAACTCGGAGT
  TCCAGGCACCTTTTCTAACACCTTCGCTTTTGCCGGTGCTGGTACTCAATTCCCAGGAGCAGAAAGTCAC
  CCCCACACCCAGTAAATTGGAGCCAGCTTCCCTCCCAAATCCTCTAGGCACACGGGGGCCTTGGGTGTTT
  AACACCTGTGGCGCCAGCGGCAGGAGCGGACCCACACAAACACAGTGCGATGGGGCATACACAGGAAGCA
  GCGTGATGGTGACAGTGGGGGCTGCCGGGCCGCTCAAAGGCGTGCAGCTGTGGCGGGCGCCAGACACAGG
  CCAGTATCTGATCTCCGCCTACGGAGCGGCGGGCGGCAAGGGCGCCCAAAACCACCTGTCATGGGCGCAC
  GGCATCTTCCTCTCAGCAGTCTTCTTCCTCCGTCGCGGGGAGCCGGTGTACATCCTTGTCGGGCAGCAGG
  GCCAGGACGCCTGTCCCGGAGGGAGCCCTGAGAGCCAACTCGTCTGTCTGGGAGAGTCTGGGGAGCATGC
  AACCACCTATGGGACCGAAAGGATCCCAGGCTGGAGACGCTGGGCCGGCGGGGGCGGGGGTGGCGGAGGC
  GCCACCTCCATCTTCCGGCTGCGCGCGGGGGAGCCAGAGCCGCTGCTGGTGGCGGCGGGAGGCGGCGGGA
  GGTCCTACCGGAGGCGACCTGACCGCGGCCGGACTCAGGCCGTCCCCGAGAGGCTGGAGACCCGCGCGGC
  GGCGCCGGGCAGCGGCGGGAGAGGAGGCGCGGCAGGCGGTGATACTTCTGAGTCTGACCTCCTCTGGGCT
  GATGGGGAAGATGGCACATCCTTTGTCCACCCCAGTGGTGAGCTCTACCTACAGCCTCTGGCAGTCACAG
  AGGGCCATGGGGAGGTGGAGATCCGAAAGCATCCCAACTGCAGTCACTGCCCTTTCAAAGACTGCCAGTG
  GCAGGCAGAGCTCTGGACGGCCGAATGCACGTGCCCAGAGGGCACGGAGCTAGCTGTGGATAATGTCACT
  TGCATGGACCTGCCAACCACCGCAAGCCCTCTGATCCTGATGGGAGCTGTAGTGGCAGCCTTGGCACTGA
  GTCTCCTAATGATGTGTGCAGTCCTGATTCTAGTGAACCAGAAGTGTCAGGGCCTGTGGGGGACCAGGCT
  GCCAGGCCCTGAGCTTGAGCTAAGCAAGCTTCGATCCTCTGCCATCAGGACAGCACCCAACCCTTACTAT
  TGTCAGGTGGGACTCAGTCCTGCCCAGCCCTGGCCTTTGCCCCCAGGGCTCACTGAGGTTTCACCAGCCA
  ATGTCACTCTACTCAGAGCCCTTGGCCATGGTGCCTTTGGGGAAGTGTACGAGGGACTAGTGACTGGTCT
  TCCTGGGGACTCCAGTCCTCTTCCAGTGGCTATTAAGACTCTGCCAGAGCTCTGCTCCCATCAGGATGAG
  CTGGATTTTCTCATGGAGGCTCTGATCATCAGCAAGTTCAGCCATCAGAACATTGTACGCTGTGTGGGGC
  TCAGCTTTCGGTCTGCCCCGCGCCTCATTCTGCTGGAGCTGATGTCTGGTGGGGACATGAAGAGCTTTTT
  GAGGCACAGCAGACCACACCCAGGACAACTGGCACCTCTGACCATGCAGGACCTATTGCAGCTGGCCCAG
  GATATAGCCCAGGGCTGCCACTACCTGGAGGAAAATCACTTCATTCACAGAGACATTGCTGCCCGTAACT
  GTCTGCTTAGCTGCAGTGGAGCCAGCCGAGTGGCCAAGATTGGAGATTTTGGAATGGCAAGAGATATCTA
  CCAGGCCAGTTATTATCGCAAGGGTGGCCGGACCTTGCTCCCAGTCAAGTGGATGCCGCCAGAAGCTCTC
  CTGGAGGGCCTTTTCACATCCAAGACAGACTCCTGGTCTTTTGGGGTCCTGCTCTGGGAGATCTTCTCAC
  TGGGGTATATGCCCTACCCTGGACATACCAACCAGGAGGTTCTAGACTTCATTGCCACAGGGAACAGGAT
  GGACCCTCCTAGGAACTGTCCTGAGCCAGTGTACCGAATCATGACCCAGTGTTGGCAGCATCAGCCGGAG
  CTCCGCCCTGACTTTGGCAGCATCTTGGAACGGATTCAGTACTGCACTCAGGACCCTGATGTGCTGAACT
  CACCCCTGCCCGTGGAACCTGGGCCCATTCTAGAGGAGGAAGAGGCCTCCAGGCTGGGAAACAGGTCACT
  GGAGGGTCTTAGATCCCCAAAGCCCCTAGAGCTGAGTTCTCAGAACTTGAAGAGCTGGGGAGGAGGCCTT
  CTTGGCTCTTGGCTGCCCTCTGGCCTCAAGACCCTCAAACCCAGGTGCCTCCAACCTCAGAACATTTGGA
  ACCCCACCTATGGCTCCTGGACCCCAAGGGGCCCCCAGGGTGAAGATACAGGCATTGAACAGTGCAATGG
  CTCCTCCTCAAGTTCCATTCCAGGCATCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222630 representing NM_206942
  Red=Cloning site Green=Tags(s)
  
  MGCSHRLLLWLGAAGTILCSNSEFQAPFLTPSLLPVLVLNSQEQKVTPTPSKLEPASLPNPLGTRGPWVF
  NTCGASGRSGPTQTQCDGAYTGSSVMVTVGAAGPLKGVQLWRAPDTGQYLISAYGAAGGKGAQNHLSWAH
  GIFLSAVFFLRRGEPVYILVGQQGQDACPGGSPESQLVCLGESGEHATTYGTERIPGWRRWAGGGGGGGG
  ATSIFRLRAGEPEPLLVAAGGGGRSYRRRPDRGRTQAVPERLETRAAAPGSGGRGGAAGGDTSESDLLWA
  DGEDGTSFVHPSGELYLQPLAVTEGHGEVEIRKHPNCSHCPFKDCQWQAELWTAECTCPEGTELAVDNVT
  CMDLPTTASPLILMGAVVAALALSLLMMCAVLILVNQKCQGLWGTRLPGPELELSKLRSSAIRTAPNPYY
  CQVGLSPAQPWPLPPGLTEVSPANVTLLRALGHGAFGEVYEGLVTGLPGDSSPLPVAIKTLPELCSHQDE
  LDFLMEALIISKFSHQNIVRCVGLSFRSAPRLILLELMSGGDMKSFLRHSRPHPGQLAPLTMQDLLQLAQ
  DIAQGCHYLEENHFIHRDIAARNCLLSCSGASRVAKIGDFGMARDIYQASYYRKGGRTLLPVKWMPPEAL
  LEGLFTSKTDSWSFGVLLWEIFSLGYMPYPGHTNQEVLDFIATGNRMDPPRNCPEPVYRIMTQCWQHQPE
  LRPDFGSILERIQYCTQDPDVLNSPLPVEPGPILEEEEASRLGNRSLEGLRSPKPLELSSQNLKSWGGGL
  LGSWLPSGLKTLKPRCLQPQNIWNPTYGSWTPRGPQGEDTGIEQCNGSSSSSIPGIQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206942
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_206942.2, NP_996825.2
• RefSeq Size: 2932
• RefSeq ORF: 2484
• Locus ID: 17005
• Gene Summary: The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008]