Prg4 (NM_001110146) Mouse Tagged ORF Clone

CAT#: MG222669

Prg4 (GFP-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor articular superficial zone protein) (Prg4) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prg4
• Synonyms: CACP; DOL54; JCAP; lubricin; MSF; SZP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222669 representing NM_001110146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGGAAAATACTTCCCGTCTGCTTGTCGCTGCTGCTTCCTGTTGTCTTGATTCAACAGGTTTCAT
  CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTACTCTAGAGATGCCACCTGCAACTGTGA
  TTATAACTGTCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC
  AAAGGACGCTGCTTCGAGTCCTTCGCTCGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTATG
  GCAAGTGCTGTGCAGATTATGACAGCTTCTGTGAAGAAGTAAAAGATAACAAGAAAAACACTCCTAAAAA
  GAAACCCAATCCAGAACCGCCGGCTGTGGATGAAGCTGGAAGCGGGCTGGATAATGCTATTCCAGGCACT
  GATCTTTTGGCCGGGAGACTCAATCAAGGCATTAACATCAATCCCATGCTTTCAGATGAGACCAATTTAT
  GCAATGGTAAGCCAGTGGATGGACTGACTACGCTGCGCAATGGGACATTAGTTGCATTTCGAGGTCATTA
  TTTCTGGATGCTGAATCCATTCAGACCACCATCTCCACCACGCAGAATTACCGAAGTTTGGGGTATTCCC
  TCTCCTATTGACACAGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCTCAGT
  ACTGGCGCTTCACCAATGATGTGGTAGATCCTGGGTATCCTAAACAAATTGTCAAAGGCTTTGGAGGACT
  AACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGGCCTGAATCTGTGTACTTCTTC
  AAGAGAGGTGGCAACATTCAGCAGTACACTTATAAACAGGAGCCAATGAAGAAGTGCACAGGGAGGCGGC
  CTGCCATCAACTACTCGGTGTACGGGGAGGCAGCACAGGTCAGGAGGCGCCGCTTTGAGCGTGCTGTTGG
  ACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATGAGAGTTTCTTATCAAGACAAAGGT
  TTCCTCCACAATGAAGTCAAAGTGAGTACAATGTGGAGAGGGTTTCCAAATGTTGTTACTTCAGCGATAA
  CACTGCCTAACATTAGGAAACCTGATGGCTATGATTACTACGCCTTTTCCAAAGATCAATACTATAACAT
  TGATGTGCCTACAAGAACAGCAAGAGCAATTACCACACGTTCAGGGCAGACCCTCTCCAAAATCTGGTAC
  AACTGTCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222669 representing NM_001110146
  Red=Cloning site Green=Tags(s)
  
  MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSC
  KGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNAIPGT
  DLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIP
  SPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFF
  KRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKG
  FLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWY
  NCP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110146
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001110146.1, NP_001103616.1
• RefSeq Size: 1884
• RefSeq ORF: 1272
• Locus ID: 96875