St3gal3 (NM_009176) Mouse Tagged ORF Clone

CAT#: MG222827

St3gal3 (GFP-tagged) - Mouse ST3 beta-galactoside alpha-23-sialyltransferase 3 (St3gal3) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: St3gal3
• Synonyms: Siat3; Siat6; ST3GalIII; ST3N
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222827 representing NM_009176
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTCCTGGTCCTGGGATTTTTGT
  ATTATTCTGCCTGGAAGCTACACTTACTCCAATGGGAAGACTCCAATTCATTGCTTCTTTCCCTTGACTC
  CGCTGGACAAACCCTAGGCACAGAGTATGATAGGCTGGGCTTCCTCCTGAAGCTGGACTCTAAACTGCCT
  GCCGAGCTAGCCACCAAGTACGCAAACTTTTCCGAGGGAGCTTGCAAACCCGGCTACGCTTCAGCTATGA
  TGACTGCCATCTTCCCCAGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGC
  TAGGATCCGGGAGTTTGTGCCACCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCA
  GTCACCAAAGAATACCGCCTGACCCCTGCCTTGGACAGCCTCCACTGCCGCCGCTGCATCATTGTAGGCA
  ATGGAGGGGTCCTCGCCAACAAGTCTCTGGGGTCACGGATTGACGACTATGACATTGTGATCAGACTGAA
  CTCAGCACCTGTGAAGGGCTTTGAGAGGGACGTGGGCAGCAAGACCACCCTACGCATCACCTACCCTGAG
  GGCGCCATGCAGCGACCTGAGCAATATGAACGAGACTCTCTCTTTGTCCTCGCTGGCTTCAAGTGGCAGG
  ACTTCAAGTGGCTGAAGTACATCGTCTACAAGGAGAGAGTGAGTGCATCTGATGGCTTCTGGAAGTCCGT
  GGCCACCCGAGTGCCCAAGGAGCCCCCTGAGATCCGCATCCTCAACCCATACTTCATCCAGGAGGCTGCC
  TTCACTCTCATTGGACTGCCCTTCAACAACGGCCTCATGGGCAGAGGGAACATCCCAACCCTTGGCAGTG
  TGGCAGTGACCATGGCACTACACGGCTGTGATGAAGTGGCAGTCGCGGGCTTTGGCTATGACATGAACAC
  ACCCAATGCACCCCTGCACTACTATGAAACTGTGCGCATGGCAGCCATCAAAGAGTCCTGGACACACAAC
  ATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTAGTGAAGGCACGTGTCATCACTGACTTAAGCAGCGGTA
  TC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222827 representing NM_009176
  Red=Cloning site Green=Tags(s)
  
  MGLLVFVRNLLLALCLFLVLGFLYYSAWKLHLLQWEDSNSLLLSLDSAGQTLGTEYDRLGFLLKLDSKLP
  AELATKYANFSEGACKPGYASAMMTAIFPRFSKPAPMFLDDSFRKWARIREFVPPFGIKGQDNLIKAILS
  VTKEYRLTPALDSLHCRRCIIVGNGGVLANKSLGSRIDDYDIVIRLNSAPVKGFERDVGSKTTLRITYPE
  GAMQRPEQYERDSLFVLAGFKWQDFKWLKYIVYKERVSASDGFWKSVATRVPKEPPEIRILNPYFIQEAA
  FTLIGLPFNNGLMGRGNIPTLGSVAVTMALHGCDEVAVAGFGYDMNTPNAPLHYYETVRMAAIKESWTHN
  IQREKEFLRKLVKARVITDLSSGI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009176
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009176.4, NP_033202.3
• RefSeq Size: 2568
• RefSeq ORF: 1125
• Locus ID: 20441
• Gene Summary: Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc. [UniProtKB/Swiss-Prot Function]