Rock2 (NM_009072) Mouse Tagged ORF Clone

CAT#: MG222994

Rock2 (GFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rock2
• Synonyms: B230113H15Rik; mKIAA0619; Rho-kinase; Rock-II; Rock2m; ROKalpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222994 representing NM_009072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGGGACGGGGCGGGCG
  CCGGCCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCAACGTGGAGAGTCTGCT
  GGATGGCTTAAATTCCTTGGTCCTTGATTTGGATTTTCCTGCTTTGAGGAAAAATAAAAATATAGATAAC
  TTCTTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCGGAAGACTATGATG
  TTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTGGTTCGTCATAAGGCATCACAGAAGGT
  TTATGCAATGAAGCTTCTTAGTAAATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAA
  AGAGATATTATGGCCTTTGCCAACAGTCCCTGGGTAGTTCAGCTCTTCTGTGCCTTTCAAGATGACAGGT
  ACCTGTACATGGTGATGGAGTACATGCCAGGCGGAGACCTCGTCAACCTTATGAGTAATTATGATGTACC
  TGAAAAGTGGGCCAAGTTTTACACTGCTGAAGTAGTGCTTGCACTGGATGCAATACACTCCATGGGCTTA
  ATACACAGAGATGTGAAGCCTGATAACATGCTATTGGATAAACATGGACATCTAAAGTTAGCAGATTTTG
  GCACATGTATGAAAATGGATGAAACAGGCATGGTGCATTGCGACACAGCAGTTGGAACTCCCGACTACAT
  ATCGCCCGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGGCGAGAATGTGATTGGTGGTCTGTA
  GGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACACTCCATTTTACGCAGATTCACTTGTAGGAACCTACA
  GCAAAATTATGGATCATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGCGAAGAA
  TCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAAGAAATGGGGTAGAAGAAATCAAACAG
  CATCCTTTCTTTAAGAATGATCAATGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTACCTG
  AGCTCAGCAGTGACATAGACAGCAGCAACTTTGACGACATTGAGGATGACAAAGGCGATGTAGAGACCTT
  CCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTGCCTTTTATAGGATTTACCTACTTTAGAGAAAATTTG
  TTATTAAGTGACTCTCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAAGTCAGG
  AGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGTGAGGTGCAAGCCAAGGAGGAGCTGGA
  ACAGAAGTGCAAATCTATTAATACTCGCCTAGAGAAAACAGCAAAGGAGCTAGAAGAAGAGATTACCTTA
  CGGAAAAGTGTGGAATCAACATTAAGACAGCTGGAAAGAGAAAAGGCCCTTCTTCAGCACAAAAATGCAG
  AGTATCAGCGGAAAGCTGATCATGAAGCCGACAAGAAGCGGAACTTGGAAAATGATGTTAACAGCTTAAA
  AGATCAACTTGAAGATCTGAAGAAAAGGAACCAGAGCTCTCAGATATCCACTGAGAAGGTCAATCAGCTC
  CAGAAACAACTGGATGAAGCTAATGCTTTGCTGAGAACAGAGTCTGATACTGCAGCACGGTTAAGAAAAA
  CCCAGGCAGAAAGTTCCAAACAGATTCAGCAGCTGGAATCTAACAATAGAGATCTACAAGATAAAAACTG
  CCTGCTTGAGACTGCCAAGTTAAAACTTGAAAAGGAATTTATCAATCTTCAGTCAGCTTTAGAATCTGAA
  AGAAGGGACCGGACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATATCTGGGTTGGAAGAAG
  ATTTGAAGACTGGCAAAGCCTTACTAGCAAAAGTAGAGCTGGAGAAGAGACAGCTGCAGGAGAAGCTCAC
  TGACTTAGAGAAGGAAAAAAGCAACATGGAAATAGATATGACATATCAACTGAAAGTTATACAGCAGAGT
  TTAGAACAAGAAGAAGCTGAACACAAGACCACAAAAGCACGGCTAGCGGATAAAAATAAGATCTATGAAT
  CCATTGAAGAAGCTAAATCAGAAGCCATGAAAGAAATGGAGAAGAAGCTGTTGGAAGAGAGGTCTTTAAA
  GCAGAAAGTGGAGAACCTGCTCCTGGAAGCCGAGAAAAGATGCTCTATATTGGACTGTGACCTCAAACAG
  TCTCAGCAGAAGCTAAATGAGCTGCTGAAGCAGAAAGATGTGCTGAACGAGGATGTTAGAAATTTGACAT
  TAAAAATAGAGCAGGAAACTCAGAAGCGTTGTCTTATGCAAAATGACCTGAAGATGCAGACCCAACAAGT
  TAACACCCTAAAAATGTCAGAAAAGCAAATAAAACAAGAAAATAATCATCTTATGGAAATGAAAATGAAC
  TTGGAAAAGCAAAATACTGAACTTCGAAAAGAGCGGCAGGATGCAGATGGGCAGATGAAGGAACTGCAAG
  ACCAACTTGAAGCAGAGCAGTATTTCTCAACCCTCTATAAAACACAAGTTAGAGAACTTAAAGAAGAAAA
  TGAAGAGAAGACAAAACTTTGTAAAGAGTTGCAACAGAAGAAGCAGGATTTACAGGATGAAAGAGACTCT
  TTGGCTGCCCAGCTGGAAATTACCCTTACCAAAGCAGATTCTGAGCAGCTGGCTCGTTCCATTGCTGAAG
  AACAATACTCTGATTTGGAAAAAGAGAAGATCATGAAAGAGCTGGAGATCAAAGAGATGATGGCTAGACA
  CAAACAAGAACTTACTGAAAAGGATACTACAATTGCATCTCTTGAAGAAACAAATAGGACACTAACTAGC
  GATGTTGCAAATCTTGCAAATGAGAAAGAAGAATTAAACAACAAGCTGAAAGATAGCCAAGAACAACTGT
  CCAAGTTGAAAGATGAAGAGATGAGTGCAGCAGCTATTAAAGCACAGTTTGAGAAGCAGCTTCTGAATGA
  GAGAACTCTCAAGACTCAAGCTGTGAATAAGTTGGCGGAGATCATGAATCGAAAAGAACCTGTCAAGCGT
  GGTAGTGACACAGATGTGCGAAGAAAAGAGAAGGAAAACAGAAAGCTACATATGGAGCTTAAGTCTGAAC
  GTGAAAAATTGACACAACAGATGATCAAATATCAGAAAGAACTGAATGAAATGCAGGCTCAAATAGCTGA
  AGAGAGCCAAATTCGAATAGAACTCCAGATGACCCTGGACAGTAAAGACAGCGACATTGAACAGCTGCGG
  TCACAGCTCCAGGCCTTGCATATTGGTATGGACAGTTCCAGTATAGGCAGTGGACCAGGCGATGCTGAGC
  CTGATGATGGATTTCCAGAATCAAGATTAGAAGGATGGTTGTCATTGCCTGTGCGCAACAACACTAAAAA
  GTTTGGATGGGTTAAAAAGTATGTGATTGTAAGCAGCAAGAAGATTCTTTTTTATGACAGCGAACAAGAT
  AAAGAGCAGTCCAACCCTTACATGGTGTTAGACATAGACAAGCTATTTCACGTCCGACCTGTTACCCAGA
  CAGATGTATACAGAGCAGATGCTAAAGAAATTCCAAGGATATTTCAGATTCTATATGCCAATGAAGGAGA
  AAGTAAGAAGGAACCAGAGTTTCCAGTGGAACCAGTGGGAGAAAAATCTAATTATATTTGTCACAAAGGA
  CATGAGTTTATTCCTACTCTTTATCACTTCCCAACCAACTGTGAGGCATGTATGAAGCCGCTGTGGCACA
  TGTTTAAGCCTCCTCCTGCTCTAGAGTGCCGTAGGTGCCACATTAAATGTCATAAAGATCACATGGACAA
  GAAGGAAGAGATTATAGCGCCCTGCAAAGTTTATTATGATATATCGTCGGCAAAGAATCTGTTGTTATTA
  GCAAATTCAACAGAAGAACAGCAGAAATGGGTTAGTCGGTTGGTGAAAAAGATACCTAAAAAGCCTCCAG
  CTCCAGACCCTTTTGCCCGATCATCCCCTAGAACTTCAATGAAAATACAACAAAACCAGTCCATTCGGCG
  GCCCAGTCGACAGCTTGCTCCAAACAAGCCAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222994 representing NM_009072
  Red=Cloning site Green=Tags(s)
  
  MSRPPPTGKMPGAPETAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDN
  FLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEE
  RDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGL
  IHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSV
  GVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQ
  HPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENL
  LLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITL
  RKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQL
  QKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESE
  RRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQS
  LEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQ
  SQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMN
  LEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDS
  LAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTS
  DVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKR
  GSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLR
  SQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQD
  KEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKG
  HEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLL
  ANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009072
• ORF Size: 4164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009072.2, NP_033098.2
• RefSeq Size: 8012
• RefSeq ORF: 4167
• Locus ID: 19878