Bmp3 (NM_173404) Mouse Tagged ORF Clone

CAT#: MG223213

Bmp3 (GFP-tagged) - Mouse bone morphogenetic protein 3 (Bmp3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bmp3
• Synonyms: 9530029I04Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223213 representing NM_173404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGCGCGCGGGCTGCTCTGTCTATGGCTAGGTTACTTCTGCCTGAACCTGGCACAGGGACAGA
  GACCAAACTTGCACCTCCCGGGACTCCGTGAGACTGAGCCAAGCGACCGCGCGACAGGTGGTAGCCCGAG
  TCCGGACCTGAGGCCGCACGACAAGGTGTCGGAGCATATGCTGTGGCTCTATGACAGGTACAGCGGCAGC
  AGTAGAGTCCAGGCTACCCGGACACCGGGCTCGCAGCTCCCGGGTCCTCAGCCCCTGCGCGGTGGTAACA
  CGGTCCGCAGCTTCAGAGCCGCAGCCGCAGGAACTCCTCAAACGAAGGGGTTACACACCTTCAATCTGAC
  TTCTCTAACCAAGTCGGAGAACATTTTGTCAGCCACACTGTATTTCTACGTTGGAGAATTAGTGAACATC
  AGCTTGAGCTGTCCAGAACCCCAAGGATGCTCCCATCACACTCAGAGACAACACATCCAGATAGACCTCT
  CTGCATGGATCCTCAAATCCAACCAAAGCCAGCTCTTGGGTCATCTGTCTGTAGATGTGGTCAGACCTTA
  TAGAGACAGCGTGTCTTGGCTGTCAAAAGACATCACTCAGCTCTTAAGAAAGGCCAAGCAAAATGAAGAG
  TTTCTCATAGGGTTTAACATTACCTCCAGAGCACACGAGCTACCCAAGAGGATGCTGTTTTTCCCGGAAC
  CTTATATCTTGGTATACGCCAACGATGCTGCCATTTCTGAGCCAGAAAGCGTGGTATCCAGCTTACAGAG
  ACACCGAGATTTCACAGCGGGAACTGGGCCCAGACTGGATAGCCACGTCAGAGAAGCCCTCTCGGTTGAG
  AGGAGGAAGAAGCGCTCTACTGGGATCTTGCTGCCCTTGCAGAACAATGAGCTACCTGGGGCAGAGTATC
  AGTACAAGGAGGAGGGAGCGTGGGAGGAGAGAAAGCCTTATAAGAGCCTTCAGACTCAGCCCCCTGAGAA
  GAGTAGGAACAAAAAGAAACAGAGGAAAGGGTCCCATCAGAAGGGACAGACGCTGCAATTTGATGAGCAG
  ACCCTGAAGAAGGCAAGGCGAAAGCAGTGGGTCGAACCTCGGAACTGTGCCAGGAGGTACCTTAAAGTGG
  ACTTTGCTGATATCGGCTGGAGCGAATGGATTATCTCTCCCAAGTCATTTGATGCTTTCTACTGCTCTGG
  AGCCTGCCAGTTCCCCATGCCAAAGTCTTTGAAACCATCAAATCACGCCACCATCCAGAGCATAGTGCGA
  GCGGTGGGGGTCGTCTCCGGGATTCCCGAGCCTTGCTGTGTGCCGGAAAAGATGTCCTCACTCAGCATCT
  TGTTCTTTGATGAAAACAAGAATGTAGTGCTCAAAGTCTACCCTAACATGACAGTCGACTCCTGTGCTTG
  TAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223213 representing NM_173404
  Red=Cloning site Green=Tags(s)
  
  MAGARGLLCLWLGYFCLNLAQGQRPNLHLPGLRETEPSDRATGGSPSPDLRPHDKVSEHMLWLYDRYSGS
  SRVQATRTPGSQLPGPQPLRGGNTVRSFRAAAAGTPQTKGLHTFNLTSLTKSENILSATLYFYVGELVNI
  SLSCPEPQGCSHHTQRQHIQIDLSAWILKSNQSQLLGHLSVDVVRPYRDSVSWLSKDITQLLRKAKQNEE
  FLIGFNITSRAHELPKRMLFFPEPYILVYANDAAISEPESVVSSLQRHRDFTAGTGPRLDSHVREALSVE
  RRKKRSTGILLPLQNNELPGAEYQYKEEGAWEERKPYKSLQTQPPEKSRNKKKQRKGSHQKGQTLQFDEQ
  TLKKARRKQWVEPRNCARRYLKVDFADIGWSEWIISPKSFDAFYCSGACQFPMPKSLKPSNHATIQSIVR
  AVGVVSGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVDSCACR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173404
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173404.3, NP_775580.1
• RefSeq Size: 3112
• RefSeq ORF: 1407
• Locus ID: 110075
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein suppresses osteoblast differentiation, and negatively regulates bone density, by modulating TGF-beta receptor availability to other ligands. Homozygous knockout mice for this gene exhibit increased bone density and volume, while overexpression of this gene in a transgenic mouse causes bone defects resulting in spontaneous rib fractures. This gene encodes distinct protein isoforms that may be similarly proteolytically processed. [provided by RefSeq, Jul 2016]