Ago1 (NM_153403) Mouse Tagged ORF Clone

CAT#: MG223226

Ago1 (GFP-tagged) - Mouse eukaryotic translation initiation factor 2C 1 (Eif2c1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ago1
• Synonyms: Eif2c1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223226 representing NM_153403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCGGGACCCTCGGGAGCAGCGGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTTCAGGCAC
  CCCGCCGGCCTGGCATTGGCACTGTGGGCAAACCAATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT
  TCCTAAGATTGACGTTTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG
  GAGGTGGTGGAATACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTATGATG
  GAAAGAAGAATATTTACACGGTCACAGCACTGCCCATTGGCAATGAGAGGGTTGACTTTGAGGTGACAAT
  CCCCGGGGAGGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGCTGGCGCATG
  CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCCTGGAGTCTGTGCAAGCCCTGGATGTGGCCA
  TGAGGCACCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA
  CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG
  ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG
  AGGTCCTGGACATCAGGAACATAGACGAACAACCCAAGCCCCTCACGGATTCCCAGCGTGTTCGGTTTAC
  CAAGGAGATAAAAGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT
  AATGTTACCCGCCGCCCTGCTAGCCATCAGACGTTCCCCTTGCAGCTGGAGAGTGGACAGACTGTGGAGT
  GCACTGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTACA
  AGTTGGGCAAGAACAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC
  ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC
  AGGAGGAGATCAGCCGCCTGATGAAGAATGCTAGCTACAACCTGGATCCCTACATCCAGGAATTTGGAAT
  CAAAGTGAAGGATGACATGACGGAGGTGACAGGACGTGTGCTGCCGGCGCCCATCTTGCAGTATGGCGGC
  CGGAACCGGGCCATTGCTACGCCCAACCAAGGTGTCTGGGACATGCGCGGGAAACAGTTCTACAATGGGA
  TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAGAAACAGTGTCGAGAAGAGGTGCTCAAGAA
  CTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGCTTCTGC
  AAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACTCAGGACTGC
  AGCTCATTATCGTCATCCTGCCCGGGAAGACGCCAGTGTATGCTGAAGTGAAACGTGTCGGAGATACACT
  CTTGGGAATGGCAACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACGTCACCGCAGACTCTGTCCAAC
  CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCTG
  TTTTTCAACAGCCAGTGATTTTCCTGGGAGCAGATGTTACACATCCCCCAGCTGGGGATGGGAAGAAACC
  ATCTATCACAGCAGTGGTAGGCAGCATGGACGCACACCCCAGCCGATACTGTGCCACCGTGCGTGTGCAG
  CGCCCACGGCAGGAGATCATTGAAGACTTGTCCTATATGGTGCGCGAGCTGCTTATCCAGTTCTACAAGT
  CCACCCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGACGGCGTCCCCGAGGGCCAGCTGCCCCAGAT
  TCTTCACTATGAGCTGCTTGCCATTCGAGATGCATGCATCAAACTGGAAAAAGACTACCAGCCTGGGATC
  ACATATATTGTGGTGCAGAAGCGCCATCACACCCGCCTGTTCTGTGCTGACAAGAATGAGCGGATTGGGA
  AGAGTGGTAACATCCCAGCAGGGACCACTGTGGACACCAACATCACCCACCCATTTGAGTTCGACTTCTA
  TCTGTGCAGCCATGCAGGCATCCAGGGTACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC
  CGTTTCACAGCAGATGAACTCCAGATCTTGACATACCAGCTGTGCCACACTTACGTACGATGCACACGTT
  CTGTCTCTATCCCAGCACCTGCCTACTATGCTCGCTTGGTGGCTTTCCGGGCACGATACCACCTGGTGGA
  CAAGGAACATGACAGTGGAGAGGGGAGCCACATATCAGGGCAAAGCAATGGGCGAGACCCCCAGGCCCTG
  GCCAAAGCTGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223226 representing NM_153403
  Red=Cloning site Green=Tags(s)
  
  MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNR
  EVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRM
  LHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWK
  MMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVC
  NVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRC
  IKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGG
  RNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFC
  KYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSN
  LCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQ
  RPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGI
  TYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDN
  RFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQAL
  AKAVQVHQDTLRTMYFA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153403
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153403.2, NP_700452.2
• RefSeq Size: 7065
• RefSeq ORF: 2574
• Locus ID: 236511
• Gene Summary: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]