Eif4g1 (NM_001005331) Mouse Tagged ORF Clone
CAT#: MG223361
- TrueORF®
Eif4g1 (GFP-tagged) - Mouse eukaryotic translation initiation factor 4 gamma 1 (Eif4g1) transcript variant 2, (10ug)
"NM_001005331" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Eif4g1 |
Synonyms | E030015G23Rik; eIF4GI |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223361 representing NM_001005331
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC CCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCC CCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGA TCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCGTTCCACATATGTTGTCCCAAC ACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACCGAGTTTGGGACC TATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTAGTGTGGCTCCTGCCCCAGTTT TGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTATCCGAATTCGAGACCCAAACCA AGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACTCCTCCCCAG ACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTGCTGTCATTATCCGGCCAGATG ACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCCAGAGCATAGCCCTGGCACAGA ATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTGGAGCCGGGGTCTGAGTCTAAT CTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATACCAATGTCTGTAGAAGAATCGA CCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACCCACTCTTGCCGAACCCATACT GGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCTTCCAGTCCTCTCCAGGTTTCC ACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGATCCCATCTGAGGATCTGGAAC CAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTGTGCTTCTGAATCGCTCGTGCC CATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCACCACCAGCTGTGGATTTAAGC CCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCACTGGCCAGCATTCTCTCTCCTG CTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAATGGAAGAAGATGACGATGACGA AGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTTGACAGTACTCCTGTCCCAGCC CAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGTCTGTGCCAAAGAGGAGACGGA AAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGCCTTCAAGGAGGTGGACCCAGC AGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCAGAGTCTGAGGGCAGCATGGTG CCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACAAAATTCATAATGCTGAGAACA TCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCCTCTAAACCTTGAAGAAAAGAA GCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGCATGCAGAAGCCAGAAGGATTG CCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTCGGCAACTGGATCCCTCCAGAC TACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCTTGGCCGACCAGCCCTCAGCAA CCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCGGCTGGCCTGGGACCCAGGCGC TCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGATAATGACTGAAGACATAAAAC TAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGCTGATAAGGATCGAGGGGAAGA GGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCCATCCTGAATAAGCTGACACCC CAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACACGGAGGAACGCCTCAAAGGAG TTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGTGGCTTATGCCAACATGTGCCG CTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACTGTGAATTTTCGAAAACTGTTG TTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAGTTTTTGAAAAAAAGCAAAAAG AGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGAGCTAGAAGAAGCCCGGGACAT AGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTCAAGTTGAAGATGTTAACAGAA GCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGGAGTCCCTGGAATGCCTCTGCC GCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCCTCGAATGGATCAGTATTTCAA CCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGTTTTATGCTGCAGGATGTACTC GATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTAAGACTATTGATCAAATTCACA AGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCTCATGGCCAAGGGCAGCGACAA ACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTTGTAGATGATGGTGGCTGGAAT ACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCACCAAGATCACAAAGCCTGGTT CCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTGGGGCAAGGGCAGCAGTGGGGG CTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCTACCTTGAATCGCTTTTCTGCT CTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTACAGAGGAGTAGCTTGAGCCGGG AACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGAGCGGGGAGGTGACCGTGGGGA CCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAAGAAGTGGAGGAGCGAAGTAGA GAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAGAGGATCGTGGTCGGGATCCTG TGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCTGTCTGTGGATGAGGTGGAGAA GAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAGGAGGCAGTACAGTGTGTCCAG GAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCGAGTCCACGCTGGAGCGTAGTA CCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGCGGGGCACCTCTCTACTGCCCA GTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAAATTGATATCCCTCATGTATGG CTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGCCCATGGGAGAGCTCTTCAGGG AAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCTGGAGATCCTGGGTCTCTTATG CAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGGCTGAGCTGGAGGGAATTTCTA GCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAATATACCTTGGGAGAAGAATCTG AAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCTAGAGAAGCTGCTGAAGGACGG CGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAGCAGCAGATAGCATCCAATACA TTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTGAGACTCCTCTCCGAGTGGATG TTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGATGAGCAGAAGGAGCTACAAGC ACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAACCTGCTTCGGATGTTCTTTGAT GCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGGAGAGCAGCAAGGATCCTGCTG AACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAATTGGCTTCGTGAGGCTGAGGA CGAGGAGTCTGATCACAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223361 representing NM_001005331
Red=Cloning site Green=Tags(s) MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAA PARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGT YAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQ TGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESN LGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVS TALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLS PVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPA QLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMV PTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGL PHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRR SQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTP QMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLL LNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTE AIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVL DLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWN TVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSA LQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSR ERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQ ELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVW LYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFL AEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNT LVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFD ALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001005331 |
ORF Size | 4779 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001005331.1, NP_001005331.1 |
RefSeq Size | 5460 bp |
RefSeq ORF | 4782 bp |
Locus ID | 208643 |
Cytogenetics | 16 B1 |
Gene Summary | This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224726 | Eif4g1 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2, (10ug) |
USD 2,150.00 |
|
MR223361 | Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2 |
USD 1,670.00 |
|
MR223361L3 | Lenti ORF clone of Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2 |
USD 1,870.00 |
|
MR223361L4 | Lenti ORF clone of Eif4g1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2 |
USD 1,870.00 |
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