Itgae (NM_172944) Mouse Tagged ORF Clone

CAT#: MG223560

Itgae (GFP-tagged) - Mouse integrin alpha E epithelial-associated (Itgae) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itgae
• Synonyms: A530055J10; alpha-E1; alpha-M290; aM290; CD103
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223560 representing NM_172944
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGGCTCTTCCATACCCTTCTCTGCATGGCCAGCCTGAAGCCCCAGGGAGCCTTCAATATGGATG
  TGGACTGGGCCTGGGTCACAGCACTCCAGCCAGGTGCACCGGCTGTGCTCAGCTCCCTTCTGCATCAAGA
  CCCCAGCAACAACCAGACCTGCCTCCTGGTAGCCAGACGCAGCTCCAACAGGAATACAGCCGCCCTGTAT
  CGATGCGCTATATCCATCAGTCCAGATGAAATTGCTTGCCAGCCAGTGGAGCACATCTGTATGCCAAAGG
  GCAGGTACCAGGGAGTGACATTGGTCGGGAACCACAATGGTGTTTTGGTGTGCATTCAGGTACAGGCCCG
  GAAGTTCCGCAGCCTCAACTCAGAGCTCACGGGTGCCTGCAGCCTACTCACCCCCAATTTGGACCTCCAG
  GCCCAAGCCTACTTCTCTGACCTCGAAGGGTTCCTGGATCCAGGAGCACATGTGGACTCTGGTGACTACT
  GTAGAAGCAAAGGAGGCAGCACAGGGGAGGAGAAGAAGTCAGCCAGGAGACGCCGGACAGTGGAGGAGGA
  AGACGAGGAGGAAGATGGCACTGAGATCGCTATTGTCCTGGATGGCTCAGGAAGCATTGAACCCTCAGAC
  TTCCAGAAGGCCAAAAATTTCATCTCTACCATGATGAGGAACTTCTATGAGAAGTGTTTTGAGTGCAACT
  TTGCCCTGGTGCAGTATGGGGCCGTGATCCAGACTGAGTTTGATCTTCAAGAGAGCCGGGACATTAACGC
  CTCCCTTGCCAAAGTTCAGAGCATCGTTCAAGTGAAGGAAGTCACTAAGACCGCCTCAGCCATGCAGCAT
  GTCCTAGACAATATCTTCATTCCAAGCCGTGGCTCCAGAAAGAAAGCATTGAAGGTCATGGTGGTACTTA
  CTGATGGTGACATATTTGGTGACCCTCTCAATCTTACGACTGTCATCAACTCCCCAAAGATGCAGGGTGT
  TGTGCGCTTTGCCATTGGGGTGGGTGATGCATTCAAGAATAACAACACTTACAGAGAACTGAAGCTGATT
  GCCTCAGACCCCAAAGAGGCCCACACTTTCAAGGTGACCAACTACTCAGCTCTGGATGGGCTGCTGAGCA
  AGCTGCAACAACACATCGTTCATATGGAAGGTACAGTTGGAGATGCCCTTCAATATCAACTCGCACAGAC
  TGGTTTCAGTGCCCAGATCCTGGACAAGGGGCAGGTGTTGCTTGGCACTGTGGGGGCCTTTAACTGGTCT
  GGGGGTGCGCTGCTCTACAGCACACAGAATGGCCGGGGCTGCTTCCTAAACCAGACAGCCAAGGAGGACT
  CCAGGACTGTACAGTACAGCTACCTAGGTTACTCCCTTGCGGTGCTACACAAGGCCCATGGCATCTCTTA
  CGTGGCAGGGGCTCCAAGGCACAAGCTCCGTGGGGCTGTGTTTGAGCTCCGGAAGGAGGACAGAGAGGAG
  GATGCCTTTGTGCGACGGATAGAAGGAGAGCAGATGGGGTCCTACTTTGGCTCTGTGCTGTGCCCTGTGG
  ATATCGACATGGATGGGACCACAGATTTTCTACTGGTGGCTGCTCCATTTTACCACATCCGTGGAGAAGA
  AGGCAGAGTCTATGTGTACCAGGTGCCTGAGCAGGATGCTTCCTTCTCCTTGGCACACACACTGAGTGGG
  CACCCTGGGCTCACCAATAGCCGCTTTGGCTTTGCCATGGCAGCTGTGGGGGATATCAACCAAGACAAAT
  TCACAGATGTGGCCATTGGGGCCCCTCTGGAGGGTTTTGGGGCAGGTGACGGGGCCAGCTATGGCAGCGT
  GTACATCTACAACGGACACTCAGGTGGCTTGTATGACAGTCCTTCCCAGCAGATCCGAGCCTCCTCTGTG
  GCCTCGGGACTCCACTACTTCGGCATGTCTGTGTCCGGTGGGTTAGATTTCAATGGCGATGGCCTTGCCG
  ATATCACAGTTGGCTCTCGGGACTCGGCGGTTGTGCTCCGCTCACGCCCTGTGGTTGACCTGACCGTATC
  TATGACCTTTACCCCCGATGCACTGCCCATGGTCTTCATAGGCAAGATGGATGTAAATCTGTGTTTTGAA
  GTTGACTCTTCAGTCGTAGCTTCTGAGCCAGGCCTTAGAGAGATGTTCCTCAACTTTACGGTGGATGTGG
  ATGTGACCAAGCAAAGGCAGAGGCTGCAGTGTGAGGACTCGAGCGGTTGTCAGAGCTGCCTCAGGAAGTG
  GAACGGAGGGTCCTTTCTGTGTGAACACTTCTGGCTCATCTCCACAGAGGAGCTTTGTGAGGAGGACTGC
  TTCTCCAACATCACCATCAAGGTCACCTACGAGTTCCAGACGTCTGGGGGAAGGAGGGACTACCCCAACC
  CCACCCTGGACCACTACAAGGAACCCTCTGCCATCTTCCAGCTGCCCTACGAGAAGGACTGCAAGAACAA
  AGTGTTCTGCATCGCTGAGATCCAGCTGACCACCAACATCTCCCAGCAGGAACTGGTGGTGGGCGTCACA
  AAGGAGGTGACCATGAACATCAGCCTGACTAACTCTGGAGAGGATTCCTACATGACAAACATGGCTCTCA
  ATTATCCCAGAAACTTACAGTTTAAGAAGATACAAAAGCCAGTATCTCCAGATGTTCAGTGTGATGACCC
  CAAGCCAGTTGCTTCTGTCCTGGTCATGAACTGCAAGATTGGTCACCCCATACTCAAGAGATCATCTGTG
  AATGTCTCAGTAACTTGGCAGCTAGAGGAGAGTGTCTTTCCAAATAGGACAGCAGATATCACTGTGACCA
  TCTCCAATTCCAATGAGAAGTCTTTGGCCAGAGAGACACGCAGCCTTCAATTCAGACATGCCTTCATTGC
  AGTTCTTTCCAGACCCTCAGTGATGTACATGAACACAAGCCAAAGCCCTTCTGACCACAAAGAATTCTTC
  TTCAATGTGCATGGGGAGAATCTTTTTGGAGCTGTATTCCAGTTGCAAATTTGTGTCCCCATCAAATTAC
  AAGATTTCCAGATTGTAAGAGTGAAGAATCTGACAAAAACTCAGGTATTTAAATGGGAGTCTGTGATTGC
  ATGGCTTTCCAAAGACTACAGATTCTCAAAGATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223560 representing NM_172944
  Red=Cloning site Green=Tags(s)
  
  MKWLFHTLLCMASLKPQGAFNMDVDWAWVTALQPGAPAVLSSLLHQDPSNNQTCLLVARRSSNRNTAALY
  RCAISISPDEIACQPVEHICMPKGRYQGVTLVGNHNGVLVCIQVQARKFRSLNSELTGACSLLTPNLDLQ
  AQAYFSDLEGFLDPGAHVDSGDYCRSKGGSTGEEKKSARRRRTVEEEDEEEDGTEIAIVLDGSGSIEPSD
  FQKAKNFISTMMRNFYEKCFECNFALVQYGAVIQTEFDLQESRDINASLAKVQSIVQVKEVTKTASAMQH
  VLDNIFIPSRGSRKKALKVMVVLTDGDIFGDPLNLTTVINSPKMQGVVRFAIGVGDAFKNNNTYRELKLI
  ASDPKEAHTFKVTNYSALDGLLSKLQQHIVHMEGTVGDALQYQLAQTGFSAQILDKGQVLLGTVGAFNWS
  GGALLYSTQNGRGCFLNQTAKEDSRTVQYSYLGYSLAVLHKAHGISYVAGAPRHKLRGAVFELRKEDREE
  DAFVRRIEGEQMGSYFGSVLCPVDIDMDGTTDFLLVAAPFYHIRGEEGRVYVYQVPEQDASFSLAHTLSG
  HPGLTNSRFGFAMAAVGDINQDKFTDVAIGAPLEGFGAGDGASYGSVYIYNGHSGGLYDSPSQQIRASSV
  ASGLHYFGMSVSGGLDFNGDGLADITVGSRDSAVVLRSRPVVDLTVSMTFTPDALPMVFIGKMDVNLCFE
  VDSSVVASEPGLREMFLNFTVDVDVTKQRQRLQCEDSSGCQSCLRKWNGGSFLCEHFWLISTEELCEEDC
  FSNITIKVTYEFQTSGGRRDYPNPTLDHYKEPSAIFQLPYEKDCKNKVFCIAEIQLTTNISQQELVVGVT
  KEVTMNISLTNSGEDSYMTNMALNYPRNLQFKKIQKPVSPDVQCDDPKPVASVLVMNCKIGHPILKRSSV
  NVSVTWQLEESVFPNRTADITVTISNSNEKSLARETRSLQFRHAFIAVLSRPSVMYMNTSQSPSDHKEFF
  FNVHGENLFGAVFQLQICVPIKLQDFQIVRVKNLTKTQVFKWESVIAWLSKDYRFSKI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172944
• ORF Size: 3114 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172944.2, NP_766532.1
• RefSeq Size: 3813
• RefSeq ORF: 3117
• Locus ID: 16407
• Gene Summary: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall. [UniProtKB/Swiss-Prot Function]