Chpt1 (NM_144807) Mouse Tagged ORF Clone

CAT#: MG223567

Chpt1 (GFP-tagged) - Mouse choline phosphotransferase 1 (Chpt1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chpt1
• Synonyms: MGC28885
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223567 representing NM_144807
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGCGCCGGGGCCAGGCCGGCGCCGCGCTGGGTAAAGGCGCTAGGTGAGCCGCTCAGTGCCG
  CGCAGCTGCGGCGGCTGGAGGAGCACCGCTACACCGCGGTGGGAGAGTCGCTGTTCGAGCCGCCGCTGCA
  GCTTTACTGGACCTGGCTGCTCCAATGGATCCCGCTCTGGATGGCCCCCAACACCATCACCCTCATCGGC
  CTCGCCATCAACCTGGTCACCACACTAGTGCTCATCTTCTACTGCCCTACAGTCACGGAGGAGGCACCAT
  ACTGGACATACCTTTTATGTGCCCTGGGACTCTTTATCTACCAGTCACTGGATGCCATTGATGGGAAACA
  AGCCAGAAGGACAAACTCTTGCTCTCCCTTAGGGGAACTATTTGATCATGGTTGTGACTCTCTTTCCACA
  GTATTTATGGCCATCGGCGCTTCCATTGCTGTTCGCCTAGGAACACATCCTGACTGGTTGTTTTTCTGCT
  CTTTCGTTGGGATGTTCATGTTTTACTGTGCTCATTGGCAGACTTACGTCTCAGGAGTGTTAAGATTTGG
  AAGGGTGGATGTCACTGAGATCCAGGTAGCTTTAGTGATCGTCTTCATGTTGTCAACATTTGGAGGAGCA
  ACAATGTGGGACTATACGATACCTATTCTAGAAATAAAACTGAAGATTGTTCCGGTTCTTGGAGTTGTAG
  GTGGATTAATATTTTCCTGTTCAAATTATTTCCATGTGATCCTCCATGGTGGTGTTGGCAAGAATGGGTC
  TACTATTGCAGGCACCAGTGTCTTGTCACCTGGACTCCACATAGGATTAATTATTATATTGGCAATAATG
  ATCTATAAGAAGTCTGCAACAAATATGTTTGAAAAACATCCTTGCCTTTATACTTTAATGTTTGGATGTG
  TCTTTGCTAAAGTTGCACAAAAATTGGTGATAGCTCACATGACGAAAAGTGAACTATATCTTCAAGACAC
  TGTCTTTATTGGGCCAGGTCTTTTATTTTTAGACCAATACTTTAATAATTTTATAGATGAATATGTTGTT
  CTGTGGATAGCAATGGTCATTTCTTCATTTGATATGATGATATACTTTACTTCTTTGTGCCTGCAAATTT
  CAAGACACCTTCATCTAAACATCTTCAAGACTTCATGTCAACAAGCACCGGAACAGGTTTACAAGCATAT
  TGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223567 representing NM_144807
  Red=Cloning site Green=Tags(s)
  
  MAAGAGARPAPRWVKALGEPLSAAQLRRLEEHRYTAVGESLFEPPLQLYWTWLLQWIPLWMAPNTITLIG
  LAINLVTTLVLIFYCPTVTEEAPYWTYLLCALGLFIYQSLDAIDGKQARRTNSCSPLGELFDHGCDSLST
  VFMAIGASIAVRLGTHPDWLFFCSFVGMFMFYCAHWQTYVSGVLRFGRVDVTEIQVALVIVFMLSTFGGA
  TMWDYTIPILEIKLKIVPVLGVVGGLIFSCSNYFHVILHGGVGKNGSTIAGTSVLSPGLHIGLIIILAIM
  IYKKSATNMFEKHPCLYTLMFGCVFAKVAQKLVIAHMTKSELYLQDTVFIGPGLLFLDQYFNNFIDEYVV
  LWIAMVISSFDMMIYFTSLCLQISRHLHLNIFKTSCQQAPEQVYKHID
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144807
• ORF Size: 1194 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_144807.3, NP_659056.3
• RefSeq Size: 3239
• RefSeq ORF: 1197
• Locus ID: 212862