Tnnt2 (NM_001130177) Mouse Tagged ORF Clone

CAT#: MG223975

Tnnt2 (GFP-tagged) - Mouse troponin T2 cardiac (Tnnt2) transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tnnt2
• Synonyms: cTnT; Tnt
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223975 representing NM_001130177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAGGAAGACTGGAGTGAAGAAG
  AGGAGGACGAGCAAGAGGAGGCAGTGGAGGAGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGA
  GGCTGAGACAGAGGAGGCCAACGTAGAAGAGGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGT
  CCAGTAGAGGACACCAAACCCAAGCCCAGCAGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCG
  ATGGAGAGAGAGTGGACTTTGATGACATCCACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGAC
  TCTGATCGAGGCTCACTTCGAGAACAGGAAGAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATC
  GAAAAGCGTCGGGCAGAGCGGGCCGAGCAGCAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACC
  GCCTGGCTGAAGAGAGGGCCCGGCGTGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAA
  GAAGAAGGCTCTGTCCAACATGATGCACTTTGGAGGGTACATCCAGAAGACAGAGCGGAAGAGTGGGAAG
  AGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAGGAGGAAGGCGCTGGCCATCGACCACC
  TGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAGAGTATTCACAACCTGGAGGCTGAGAA
  GTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCAACGTTCTGCGAAACCGGATCAATGAC
  AACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGGGCGTTGGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223975 representing NM_001130177
  Red=Cloning site Green=Tags(s)
  
  MSDAEEVVEEYEEEQEEEDWSEEEEDEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEG
  PVEDTKPKPSRLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRI
  EKRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGK
  RQTEREKKKKILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRIND
  NQKVSKTRGKAKVTGRWK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130177
• ORF Size: 894 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130177.2, NP_001123649.1
• RefSeq Size: 1190
• RefSeq ORF: 897
• Locus ID: 21956
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]