Dmgdh (NM_028772) Mouse Tagged ORF Clone

CAT#: MG224057

Dmgdh (GFP-tagged) - Mouse dimethylglycine dehydrogenase precursor (Dmgdh) nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dmgdh
• Synonyms: 1200014D15Rik; AI787269
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224057 representing NM_028772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGCCCGGGCGCGCTGCGGCTGCGGGGCCTCGCGCTGCGGGGCTCCCCCAGGCGCCCAAGCTCCG
  CTGGCCTCCGGGAAGGACAGGAAAGCCCAGCCTCACCCCCAGAATGGAAGGACAGAGCAGAGACTGTGAT
  CATTGGAGGCGGCTGTGTGGGCGTGAGCCTGGCTTATCATCTGGCCAAGGCTGGGATGAGAGATGTGGTC
  CTGATGGAGAAGTCGGAGCTCACCGCTGGCTCTACCTGGCACGCAGCAGGTTTAACAACTTACTTTCACC
  CCGGGATAAACTTGAAGAAAATACATTATGACAGCATCAAACTTTACGAGAGGCTGGAAGAAGAAACCGG
  ACAGGTGGTGGGATTCCATCAACCAGGTAGTATCAGGCTTGCTACAACTCCTGTAAGGGTGGACGAATTT
  AAATACCAAATGACGCGAACCAACTGGCACGCCACGGAACAGTATATTATTGAGCCTGAAAAAATCCACG
  AGCTGTTCCCTTTACTCAACATGAACAAGATTTTAGCCGGACTGTATAACCCAGGAGATGGCCACATTGA
  TCCTTATTCTCTGACGATGGCCCTGGCTGCTGGTGCTAGGAAATATGGGGCCCTTCTAAAGTATCCAGCA
  CCAGTGACATCTCTGAAGCCCAGGCCAGATGGGACATGGGATGTGGAGACTCCACAAGGATCTGTGAGAG
  CAAATAGGATTGTGAATGCTGCAGGATTTTGGGCTCGTGAAGTGGGTAAAATGATTGGACTAGACCACCC
  CCTCATCCCCGTCCAACATCAATATGTCGTCACATCAACAATCCCAGAAGTGAAGGCTTTGAAACGAGAG
  CTCCCCGTCCTCCGCGACCTGGAAGGATCCTACTACCTCCGACAGGAAAGGGATGGGCTTTTGTTTGGTC
  CCTATGAAAGTCAGGAGAAGATGAAACTCCAAGCCTCCTGGGTCACTCACGGAGTCCCTCCAGGCTTTGG
  AAAGGAGCTTTTTGAGTCTGATTTAGATCGAATCTCAGACCACCTGGAAGCTGCCATGGAGATGATTCCT
  GTCTTGAAGAAGGCGGACATCATCAATGTCGTCAATGGCCCTATCACCTACTCTCCCGACATTCTGCCTA
  TGGTGGGGCCCCATCAGGGGGTCAGAAACTACTGGGTGGCGACTGGCTTTGGATACGGCATAATCCATGC
  TGGTGGGGTAGGCAAGTTCCTCAGTGACTGGATCCTGCATGGAGAACCTCCTTTTGACCTGATAGAACTG
  GATCCCAATCGCTACGGCAAATGGACGACGACACAGTACACCGAGGCCAAAGCCCGAGAATCATATGGGT
  TCAACAACATTGTTGGTTATCCTAAAGAAGAGCGGTTTGCTGGGAGGCCAACCCAGAGAGTCAGTGGACT
  TTACAAAACCCTGAAGTCCAAGTGTTCCATGGGCTTCCATGCAGGCTGGGAACAGCCACATTGGTTCTAT
  AAGCCTGGCCAGGACACTCAGTATAGGCCAAGTTTCCGCCGCACAAACTGGTTTGAGCCGGTGGGCTCTG
  AGTACAAACAGGTTATGCAAAGAGTTGGTGTGATTGACCTGTCTCCATTTGGCAAGTTCAACATCAAAGG
  CCGAGATTCTACCCAACTTCTGGACCATCTCTTTGCTAATGTCATTCCCAAGGTGGGTTTTACAAACATA
  AGTCACATGTTGACGCCCAGAGGTCGGGTGTATGCTGAGCTGACTGTTTCCCAGCAGTCTCCTGGGGAGT
  TCCTGTTGATCACTGGCTCCGGGTCGGAGCTTCACGACCTTAGATGGATTGAAGAAGCAGCCTTCAGAGG
  TGGATATGATGTGGAAATTCAAAACATAACCGATGAGTTTGGGGTCCTGGGAGTTGCAGGGCCATATGCA
  AGAAGAGTCCTTCAGAAGCTGACCTCAGAAGATCTCAGCGATGATGCATTCAAGTTTCTTCAAACCAAAT
  CTTTTAACATCTCAGACATTCCAGTCACTGCTATCAGAATCTCCTATACTGGTGAGCTGGGCTGGGAGCT
  GTACCACAGACGAGAAGATTCTGCGACGCTTTATGAGCGCATCATGAGTGCCGGCCAGGAGGAGGGCATC
  GGCGACTTTGGAACTTACGCCCTGAACGCTTTGAGACTGGAGAAGGCTTTCCGAGCCTGGGGCTCCGAGA
  TGAACTGTGACACAAACCCCTTGGAAGCTGGGCTGGAATATTTCGTGAAGCTGAATAAGCCCGCTGACTT
  CATAGGGAAGCAGGCCCTGAAGCAGATTAAAACCGAGGGGCTAAAGCGGAGACTGGTCTGCCTCACCGTG
  GCAACGGACGATGTGGATCCAGAGGGAAATGAAAGCATTTGGTACAAGGGCAAGGTGGTTGGCAACACAA
  CATCTGGAAGCTACAGTTACAGCATTCAGAAGAGCCTGGCTTTTGCTTATGTCCCAGTCCAGCTCAGTGA
  AGTGGGACAGCAAGTGGAGGCTGAACTACTGGGTAAAAATTACCCAGCCACCATTATACAAGAACCCCTG
  GTACTCACGGAGCCTGCCCGAGCCAGGCTTCAGAAAGACGGTAAAAAGACAAATCTTGAGAAAGGCCCAT
  CCCGGACAACCAAGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224057 representing NM_028772
  Red=Cloning site Green=Tags(s)
  
  MLRPGALRLRGLALRGSPRRPSSAGLREGQESPASPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVV
  LMEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPVRVDEF
  KYQMTRTNWHATEQYIIEPEKIHELFPLLNMNKILAGLYNPGDGHIDPYSLTMALAAGARKYGALLKYPA
  PVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVVTSTIPEVKALKRE
  LPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVTHGVPPGFGKELFESDLDRISDHLEAAMEMIP
  VLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVATGFGYGIIHAGGVGKFLSDWILHGEPPFDLIEL
  DPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKTLKSKCSMGFHAGWEQPHWFY
  KPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGRDSTQLLDHLFANVIPKVGFTNI
  SHMLTPRGRVYAELTVSQQSPGEFLLITGSGSELHDLRWIEEAAFRGGYDVEIQNITDEFGVLGVAGPYA
  RRVLQKLTSEDLSDDAFKFLQTKSFNISDIPVTAIRISYTGELGWELYHRREDSATLYERIMSAGQEEGI
  GDFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLEYFVKLNKPADFIGKQALKQIKTEGLKRRLVCLTV
  ATDDVDPEGNESIWYKGKVVGNTTSGSYSYSIQKSLAFAYVPVQLSEVGQQVEAELLGKNYPATIIQEPL
  VLTEPARARLQKDGKKTNLEKGPSRTTKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028772
• ORF Size: 2607 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028772.3, NP_083048.1
• RefSeq Size: 2979
• RefSeq ORF: 2610
• Locus ID: 74129
• Gene Summary: This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. [provided by RefSeq, Jul 2013]