Il5ra (NM_008370) Mouse Tagged ORF Clone

CAT#: MG224415

Il5ra (GFP-tagged) - Mouse interleukin 5 receptor alpha (Il5ra), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Il5ra
• Synonyms: CD125; CDw125; Il5r
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224415 representing NM_008370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCTGTGTTACTAATTCTTGTGGGAGCTTTGGCAACACTGCAAGCTGACTTACTTAATCACAAAA
  AGTTTTTACTTCTACCACCTGTCAATTTTACCATTAAAGCCACTGGATTAGCTCAAGTTCTTTTACACTG
  GGACCCAAATCCTGACCAAGAGCAAAGGCATGTTGATCTAGAGTATCACGTGAAAATAAATGCCCCACAA
  GAAGACGAATATGATACCAGAAAGACTGAAAGCAAATGTGTGACCCCCCTTCATGAAGGCTTTGCAGCTA
  GCGTGAGGACCATTCTGAAGAGCAGCCATACAACTCTGGCCAGCAGTTGGGTTTCTGCTGAACTCAAAGC
  TCCACCAGGATCTCCTGGAACCTCGGTTACGAATTTAACTTGTACCACACACACTGTTGTAAGTAGCCAC
  ACCCACTTAAGGCCATACCAAGTGTCCCTTCGTTGCACCTGGCTTGTTGGGAAGGATGCCCCTGAGGACA
  CACAGTATTTCCTATACTACAGGTTTGGTGTTTTGACTGAAAAATGCCAAGAATACAGCAGAGATGCACT
  GAACAGAAATACTGCATGCTGGTTTCCCAGGACATTTATCAACAGCAAAGGGTTTGAACAGCTTGCTGTG
  CACATTAATGGCTCAAGCAAGCGTGCTGCAATCAAGCCCTTTGATCAGCTGTTCAGTCCACTTGCCATTG
  ACCAAGTGAATCCTCCAAGGAATGTCACAGTGGAAATTGAAAGCAATTCTCTCTATATACAGTGGGAGAA
  ACCACTTTCTGCCTTTCCAGATCATTGCTTTAACTATGAGCTGAAAATTTACAACACAAAAAATGGTCAC
  ATTCAGAAGGAAAAACTGATCGCCAATAAGTTCATCTCAAAAATTGATGATGTTTCTACATATTCCATTC
  AAGTGAGAGCAGCTGTGAGCTCACCTTGCAGAATGCCAGGAAGGTGGGGCGAGTGGAGTCAACCTATTTA
  TGTGGGAAAGGAAAGGAAGTCCTTGGTAGAATGGCATCTCATTGTGCTCCCAACAGCTGCCTGCTTCGTC
  TTGTTAATCTTCTCACTCATCTGCAGAGTGTGTCATTTATGGACCAGGTTGTTTCCACCGGTTCCGGCCC
  CAAAGAGTAACATCAAAGATCTCCCTGTGGTTACTGAATATGAGAAACCTTCGAATGAAACCAAAATTGA
  AGTTGTACATTGTGTGGAAGAGGTTGGATTTGAAGTCATGGGAAATTCCACGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224415 representing NM_008370
  Red=Cloning site Green=Tags(s)
  
  MVPVLLILVGALATLQADLLNHKKFLLLPPVNFTIKATGLAQVLLHWDPNPDQEQRHVDLEYHVKINAPQ
  EDEYDTRKTESKCVTPLHEGFAASVRTILKSSHTTLASSWVSAELKAPPGSPGTSVTNLTCTTHTVVSSH
  THLRPYQVSLRCTWLVGKDAPEDTQYFLYYRFGVLTEKCQEYSRDALNRNTACWFPRTFINSKGFEQLAV
  HINGSSKRAAIKPFDQLFSPLAIDQVNPPRNVTVEIESNSLYIQWEKPLSAFPDHCFNYELKIYNTKNGH
  IQKEKLIANKFISKIDDVSTYSIQVRAAVSSPCRMPGRWGEWSQPIYVGKERKSLVEWHLIVLPTAACFV
  LLIFSLICRVCHLWTRLFPPVPAPKSNIKDLPVVTEYEKPSNETKIEVVHCVEEVGFEVMGNSTF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008370
• ORF Size: 1245 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008370.2, NP_032396.1
• RefSeq Size: 3557
• RefSeq ORF: 1248
• Locus ID: 16192
• Gene Summary: This is the receptor for interleukin-5. The alpha chain binds to IL5. [UniProtKB/Swiss-Prot Function]