Lfng (NM_008494) Mouse Tagged ORF Clone

CAT#: MG224420

Lfng (GFP-tagged) - Mouse LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Lfng), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lfng
• Synonyms: AW061165
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224420 representing NM_008494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCAGCGGTGCGGCCGGCGCCTGCTGCTGGCGCTGGTGGGCGCGCTGTTGGCTTGTCTCCTGGTGC
  TCACGGCCGACCCGCCACCGACTCCGATGCCCGCTGAGCGCGGACGGCGCGCGCTGCGTAGCCTGGCGGG
  CTCCTCTGGAGGAGCTCCGGCTTCAGGGTCCAGGGCGGCTGTGGATCCCGGAGTCCTCACCCGCGAGGTG
  CATAGCCTCTCCGAGTACTTCAGTCTACTCACCCGCGCGCGCAGAGACGCGGATCCACCGCCCGGGGTCG
  CTTCTCGCCAGGGCGACGGCCATCCGCGTCCCCCCGCCGAAGTTCTGTCCCCTCGCGACGTCTTCATCGC
  CGTCAAGACCACCAGAAAGTTTCACCGCGCGCGGCTCGATCTGCTGTTCGAGACCTGGATCTCGCGCCAC
  AAGGAGATGACGTTCATCTTCACTGATGGGGAGGACGAAGCTCTGGCCAAGCTCACAGGCAATGTGGTGC
  TCACCAACTGCTCCTCGGCCCACAGCCGCCAGGCTCTGTCCTGCAAGATGGCTGTGGAGTATGACCGATT
  CATTGAGTCTGGGAAGAAGTGGTTCTGCCACGTGGATGATGACAACTACGTCAACCTCCGGGCGCTGCTG
  CGGCTCCTGGCCAGCTATCCCCACACCCAAGACGTGTACATCGGCAAGCCCAGCCTGGACAGGCCCATCC
  AGGCCACAGAACGGATCAGCGAGCACAAAGTGAGACCTGTCCACTTTTGGTTTGCCACCGGAGGAGCTGG
  CTTCTGCATCAGCCGAGGGCTGGCCCTAAAGATGGGCCCATGGGCCAGTGGAGGACACTTCATGAGCACG
  GCAGAGCGCATCCGGCTCCCCGATGACTGCACCATTGGCTACATTGTAGAGGCTCTGCTGGGTGTACCCC
  TCATCCGGAGCGGCCTCTTCCACTCCCACCTAGAGAACCTGCAGCAGGTGCCCACCACCGAGCTTCATGA
  GCAGGTGACCCTGAGCTATGGCATGTTTGAGAACAAGCGGAACGCAGTGCACATCAAGGGACCATTCTCT
  GTGGAAGCTGACCCATCCAGGTTCCGCTCTGTCCATTGCCACCTGTACCCAGACACACCCTGGTGTCCTC
  GCTCCGCCATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224420 representing NM_008494
  Red=Cloning site Green=Tags(s)
  
  MLQRCGRRLLLALVGALLACLLVLTADPPPTPMPAERGRRALRSLAGSSGGAPASGSRAAVDPGVLTREV
  HSLSEYFSLLTRARRDADPPPGVASRQGDGHPRPPAEVLSPRDVFIAVKTTRKFHRARLDLLFETWISRH
  KEMTFIFTDGEDEALAKLTGNVVLTNCSSAHSRQALSCKMAVEYDRFIESGKKWFCHVDDDNYVNLRALL
  RLLASYPHTQDVYIGKPSLDRPIQATERISEHKVRPVHFWFATGGAGFCISRGLALKMGPWASGGHFMST
  AERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTTELHEQVTLSYGMFENKRNAVHIKGPFS
  VEADPSRFRSVHCHLYPDTPWCPRSAIF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008494
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008494.3, NP_032520.1
• RefSeq Size: 2299
• RefSeq ORF: 1137
• Locus ID: 16848
• Gene Summary: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules inhibiting Jagged mediated Notch signaling. Essential mediator of somite segmentation and patterning. During somite boundary formation, it restricts Notch activity in the presomitic mesoderm to a boundary-formig territory in the posterior half of the prospective somite. In this region, Notch function activates a set of genes that are involved in boundary formation and in anterior-posterior somite identity. Ectopically expressed in the thymus, Lfgn inhibits Notch signaling which results in inhibition of T-cell commitment and promotes B-cell development in lymphoid progenitors. May play a role in boundary formation of the enamel knot. [UniProtKB/Swiss-Prot Function]