Comp (NM_016685) Mouse Tagged ORF Clone

CAT#: MG224430

Comp (GFP-tagged) - Mouse cartilage oligomeric matrix protein (Comp), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Comp
• Synonyms: TSP5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224430 representing NM_016685.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCCCCACTGCCTGCGTTCTAGTGCTCGCCCTGGCTATCCTGCGGGCGACAGGCCAGGGCCAGATC
  CCGCTGGGTGGAGACCTGGCCCCACAGATGCTGCGAGAACTTCAGGAGACTAATGCGGCGCTGCAAGAC
  GTGAGAGAGCTGTTGCGACAGCAGGTCAAGGAGATCACCTTCCTGAAGAATACGGTGATGGAATGTGAT
  GCTTGCGGAATGCAGCCCGCACGCACTCCAGGCCTGAGCGTGCGGCCAGTGCCGCTCTGCGCACCCGGC
  TCCTGCTTCCCCGGCGTAGTCTGCTCCGAGACAGCTACGGGCGCGCGCTGCGGCCCCTGCCCTCCTGGC
  TACACCGGCAACGGCTCGCACTGCACCGACGTTAATGAGTGCAATGCTCACCCCTGTTTCCCGCGGGTG
  CGGTGCATCAATACCAGCCCTGGCTTTCACTGCGAAGCCTGTCCCCCTGGGTTCAGCGGACCCACCCAC
  GAGGGCGTGGGACTGACCTTCGCTAAGTCCAACAAACAAGTTTGCACGGATATTAATGAGTGTGAGACC
  GGGCAGCACAATTGCGTTCCCAACTCCGTGTGCGTCAACACCCGGGGCTCCTTCCAGTGCGGCCCCTGC
  CAGCCCGGTTTCGTGGGCGACCAGACGTCAGGCTGCCAGCGGCGTGGGCAGCACTTCTGCCCCGATGGG
  TCACCCAGCCCGTGCCATGAGAAAGCAAACTGCGTCCTGGAGCGGGATGGCTCGAGGTCTTGCGTGTGT
  GCAGTCGGCTGGGCCGGCAACGGGCTCCTGTGCGGCCGCGACACGGACCTGGACGGTTTTCCTGACGAG
  AAGCTTCGCTGCTCAGAGCGCCAGTGTCGCAAGGACAACTGCGTGACGGTGCCCAATTCGGGGCAGGAG
  GATGTGGACCGGGACGGCATCGGAGATGCTTGTGACCCGGATGCGGACGGGGATGGAGTCCCTAACGAG
  CAAGACAATTGCCCGCTGGTTCGAAACCCAGACCAGCGTAACTCGGACAGTGATAAGTGGGGAGATGCC
  TGCGACAACTGCCGGTCCAAGAAGAATGACGATCAGAAAGATACAGACCTGGATGGCCGGGGCGATGCC
  TGCGACGACGACATAGATGGCGACGGAATACGAAATGTAGCTGACAACTGTCCCCGGGTGCCCAACTTT
  GACCAGAGTGACAGTGATGGTGATGGTGTTGGGGATGCCTGTGACAACTGTCCCCAGAAAGATAACCCA
  GACCAGAGGGATGTGGACCACGACTTTGTGGGTGATGCCTGTGATAGTGACCAAGACCAGGATGGGGAT
  GGTCACCAGGACTCCCGGGACAACTGCCCCACAGTACCCAACAGTGCCCAGCAGGACTCAGATCATGAT
  GGCAAGGGCGATGCCTGTGATGACGATGATGACAATGACGGAGTTCCTGATAGCCGGGACAACTGCCGC
  TTGGTGCCTAACCCTGGCCAAGAGGACAATGACCGGGATGGCGTGGGTGACGCGTGTCAGGGTGACTTC
  GATGCTGACAAGGTTATAGACAAGATCGATGTGTGCCCCGAGAACGCCGAGGTCACCCTCACCGACTTC
  AGGGCCTTCCAGACGGTTGTGTTGGACCCCGAGGGTGATGCGCAGATCGATCCCAACTGGGTGGTGCTC
  AATCAGGGAATGGAGATCGTTCAGACCATGAACAGTGACCCTGGCCTGGCTGTGGGTTACACAGCCTTC
  AACGGCGTGGACTTCGAGGGCACATTCCATGTAAACACCGCCACTGATGATGACTATGCTGGTTTCATC
  TTTGGCTACCAAGACAGCTCCAGTTTCTATGTAGTCATGTGGAAACAGATGGAGCAGACGTACTGGCAG
  GCCAATCCCTTCCGGGCTGTGGCTGAGCCAGGGATTCAGCTCAAGGCTGTCAAGTCCTCTACAGGTCCC
  GGGGAACAGCTCCGAAACGCACTTTGGCACACGGGGGACACAGCATCCCAGGTGCGGCTGCTGTGGAAG
  GATCCTCGAAACGTGGGCTGGAAGGATAAAACATCCTACCGCTGGTTCCTGCAGCACCGGCCTCAAGTT
  GGCTACATCAGGGTGCGGTTCTATGAGGGTCCTGAGCTAGTAGCTGACAGCAATGTGGTGTTGGACACG
  GCCATGCGTGGTGGCCGCCTGGGTGTCTTCTGCTTCTCCCAAGAGAACATCATCTGGGCTAACCTGCGC
  TACCGTTGCAATGATACAATCCCTGAGGACTACGAGAGTCACCGGCTGCAGAGAGTC
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by MG224430
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGPTACVLVLALAILRATGQGQIPLGGDLAPQMLRELQETNAALQDVRELLRQQVKEITFLKNTVMECD
  ACGMQPARTPGLSVRPVPLCAPGSCFPGVVCSETATGARCGPCPPGYTGNGSHCTDVNECNAHPCFPRV
  RCINTSPGFHCEACPPGFSGPTHEGVGLTFAKSNKQVCTDINECETGQHNCVPNSVCVNTRGSFQCGPC
  QPGFVGDQTSGCQRRGQHFCPDGSPSPCHEKANCVLERDGSRSCVCAVGWAGNGLLCGRDTDLDGFPDE
  KLRCSERQCRKDNCVTVPNSGQEDVDRDGIGDACDPDADGDGVPNEQDNCPLVRNPDQRNSDSDKWGDA
  CDNCRSKKNDDQKDTDLDGRGDACDDDIDGDGIRNVADNCPRVPNFDQSDSDGDGVGDACDNCPQKDNP
  DQRDVDHDFVGDACDSDQDQDGDGHQDSRDNCPTVPNSAQQDSDHDGKGDACDDDDDNDGVPDSRDNCR
  LVPNPGQEDNDRDGVGDACQGDFDADKVIDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVL
  NQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTATDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQ
  ANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTASQVRLLWKDPRNVGWKDKTSYRWFLQHRPQV
  GYIRVRFYEGPELVADSNVVLDTAMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYESHRLQRV
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016685
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2447 bp
• RefSeq ORF: 2268 bp
• Locus ID: 12845
• UniProt ID: Q9R0G6
• Cytogenetics: 8 34.15 cM
• MW: 82.2 kDa
• Gene Summary: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).[UniProtKB/Swiss-Prot Function]