Chrne (NM_009603) Mouse Tagged ORF Clone

CAT#: MG224539

Chrne (GFP-tagged) - Mouse cholinergic receptor nicotinic epsilon polypeptide (Chrne), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chrne
• Synonyms: AChrepsilon; Acre; nAChRE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224539 representing NM_009603
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGGCTCTGCTTGGTGCCCTGCTTCTCCTGACACTCTTTGGCAGAAGCCAGGGAAAGAATGAAG
  AGCTTAGCCTGTATCACCATCTCTTCGACAATTATGATCCAGAATGCCGGCCAGTTAGGAGACCTGAGGA
  CACTGTCACCATCACCCTCAAGGTCACCCTAACCAACCTCATCTCACTGAACGAGAAAGAAGAAACTCTG
  ACCACCAGTGTCTGGATTGGCATTGACTGGCACGACTATCGGCTCAACTACAGCAAGGACGATTTTGCAG
  GTGTAGGAATCCTCCGGGTCCCTTCAGAACATGTATGGCTGCCAGAGATTGTTCTAGAAAACAATATTGA
  TGGGCAGTTTGGAGTGGCCTACGACAGCAATGTTCTAGTCTATGAGGGAGGCTATGTGAGCTGGTTGCCC
  CCAGCCATCTACCGCAGCACCTGCGCAGTGGAGGTCACCTATTTCCCCTTTGACTGGCAGAACTGCTCTC
  TCATTTTTCGCTCCCAGACCTACAATGCTGAGGAGGTGGAGTTCATCTTTGCCGTGGATGACGACGGCAA
  TACCATCAACAAGATTGACATTGACACGGCAGCTTTTACCGAGAATGGAGAATGGGCCATAGACTACTGC
  CCAGGCATGATTCGCCGCTATGAGGGAGGTTCCACAGAAGGTCCTGGAGAAACTGACGTCATCTATACGC
  TCATCATCCGCCGGAAGCCGCTTTTTTACGTCATTAACATCATTGTGCCTTGCGTGCTCATTTCTGGCTT
  GGTGCTGCTCGCTTACTTCCTGCCTGCGCAGGCTGGTGGCCAGAAATGCACGGTCTCTATCAACGTCCTG
  CTAGCCCAGACTGTCTTCTTGTTTCTAATTGCCCAGAAAATTCCAGAGACTTCTCTGAGCGTGCCACTGC
  TGGGCAGGTATCTTATATTCGTCATGGTGGTTGCCACGCTCATTGTCATGAATTGCGTCATCGTGCTCAA
  CGTATCTTTGAGGACGCCAACGACTCATGCTACATCCCCTCGGCTGCGCCAGATTTTATTAGAGCTGCTG
  CCGCGTCTCCTGGGCTCGAGCCCACCCCCAGAGGATCCCCGAACTGCCTCACCAGCGAGGCGTGCCTCGT
  CTGTGGGCATTCTGCTCAGAGCGGAGGAGCTCATCTTGAAAAAGCCGCGGAGCGAACTCGTGTTTGAGGG
  TCAGAGGCATCGGCACGGAACTTGGACCGCAGCCCTCTGCCAGAACCTGGGTGCTGCAGCCCCAGAAATC
  CGCTGCTGTGTGGATGCTGTGAACTTTGTGGCTGAGAGCACAAGAGACCAGGAAGCCACTGGAGAGGAAC
  TGTCCGACTGGGTGCGTATGGGGAAGGCCCTGGACAATGTCTGTTTTTGGGCAGCTTTGGTGCTCTTCAG
  CGTTGGTTCTACTCTCATCTTCCTTGGGGGTTACTTCAACCAAGTTCCTGATCTCCCTTACCCACCGTGC
  ATCCAACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224539 representing NM_009603
  Red=Cloning site Green=Tags(s)
  
  MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETL
  TTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLP
  PAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYC
  PGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVL
  LAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELL
  PRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAALCQNLGAAAPEI
  RCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPC
  IQP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009603
• ORF Size: 1479 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009603.1, NP_033733.1
• RefSeq Size: 1599
• RefSeq ORF: 1482
• Locus ID: 11448
• Gene Summary: This gene encodes the epsilon subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. In mice, deficiency of this gene can lead to a decline in the number of nicotinic acetylcholine receptors at neuromuscular junctions and causes progressive muscle weakness, atrophy and premature death. Mutations in this gene serve as a pathophysiological model for human congenital myasthenia. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Nov 2012]