Polb (NM_011130) Mouse Tagged ORF Clone

CAT#: MG224695

Polb (GFP-tagged) - Mouse polymerase (DNA directed) beta (Polb), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polb
• Synonyms: A430088C08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224695 representing NM_011130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACGCAAGGCGCCGCAGGAGACCCTCAACGGCGGCATCACGGACATGCTCGTGGAACTCGCAA
  ACTTTGAGAAGAACGTGAGCCAGGCGATCCACAAGTACAATGCGTACAGAAAAGCGGCATCTGTGATAGC
  CAAGTACCCACACAAAATCAAGAGCGGAGCGGAAGCTAAGAAACTGCCAGGAGTAGGAACAAAAATTGCT
  GAAAAGATTGATGAATTTTTAGCAACTGGAAAATTGCGTAAACTGGAAAAGATTCGTCAAGATGATACAA
  GTTCATCCATCAATTTCCTGACTCGAGTTACTGGCATTGGACCATCTGCTGCAAGGAAGTTTGTAGATGA
  AGGAATTAAGACATTAGAAGATCTCAGGAAAAATGAAGATAAACTGAACCATCATCAACGAATTGGGCTG
  AAATATTTCGAGGACTTTGAAAAGAGAATTCCTCGTGAGGAAATGCTGCAGATGCAGGATATTGTACTTA
  ATGAAATTAAAAAAGTGGACTCTGAGTACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGCGCAGAGTC
  GAGTGGAGACATGGATGTCCTGCTGACCCACCCAAACTTCACGTCAGAGTCCAGCAAACAGCCAAAGTTG
  TTACATCGTGTTGTGGAACAGTTACAAAAAGTCCATTTCATTACAGATACTCTGTCAAAGGGTGAAACAA
  AGTTCATGGGTGTTTGCCAGCTTCCCAGCGAGAAGGATGGAAAGGAATATCCACACAGGAGAATCGATAT
  CAGGTTGATCCCCAAAGATCAGTACTACTGTGGTGTTCTCTACTTCACTGGGAGTGACATCTTTAATAAG
  AACATGAGAGCGCACGCCCTGGAAAAGGGCTTCACAATCAATGAGTACACCATCCGCCCCCTGGGGGTCA
  CTGGAGTCGCTGGGGAGCCCCTTCCTGTGGACAGTGAGCAGGACATTTTTGATTACATCCAGTGGCGCTA
  CCGGGAGCCCAAGGATAGAAGTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224695 representing NM_011130
  Red=Cloning site Green=Tags(s)
  
  MSKRKAPQETLNGGITDMLVELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIA
  EKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVTGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGL
  KYFEDFEKRIPREEMLQMQDIVLNEIKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPNFTSESSKQPKL
  LHRVVEQLQKVHFITDTLSKGETKFMGVCQLPSEKDGKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK
  NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEQDIFDYIQWRYREPKDRSE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011130
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011130.2, NP_035260.1
• RefSeq Size: 1227
• RefSeq ORF: 1008
• Locus ID: 18970