Scube3 (NM_001004366) Mouse Tagged ORF Clone

CAT#: MG225155

Scube3 (GFP-tagged) - Mouse signal peptide CUB domain EGF-like 3 (Scube3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Scube3
• Synonyms: CEGF3; D030038I21Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225155 representing NM_001004366
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCGGGGCGCGTCCCCGGGCTCTGCCTGCTCCTCCTGCTGGTCCACGCGCGCGCCGCCCAGCATG
  GCAAAGCTGCGCAAGATGTAGATGAGTGTGTGGAAGGCACGGACAATTGCCACATCGATGCCATCTGCCA
  GAATACCCCACGGTCTTACAAGTGCATCTGCAAGTCCGGCTACACGGGGGACGGGAAGCACTGCAAAGAC
  GTGGATGAGTGTGAACGGGAGGACAACGCAGGCTGTGTGCACGACTGTGTCAACATCCCTGGTAATTACC
  GGTGTACCTGCTACGACGGATTTCACCTGGCACATGACGGACACAACTGTCTGGATGTGGATGAGTGTGC
  AGAGGGCAACGGTGGCTGCCAGCAGAGCTGTGTCAACATGATGGGCAGCTACGAGTGCCATTGCAGAGAT
  GGCTTCTTCCTCAGTGACAACCAGCACACCTGTATCCAGCGGCCAGAAGAAGGAATGAACTGCATGAACA
  AGAACCACGGCTGTGCCCACATTTGCCGGGAGACTCCCAAGGGGGGCATTGCCTGTGAATGCCGCCCTGG
  CTTCGAGCTCACCAAGAACCAACGGGACTGTAAATTGACCTGCAACTATGGTAATGGCGGCTGCCAGCAC
  ACCTGCGATGACACGGAGCAGGGTCCTCGGTGCGGCTGCCACGTCAAGTTTGTGCTCCACACCGACGGGA
  AGACATGCATTGAAACCTGTGCTGTCAACAACGGAGGCTGCGACAGTAAGTGCCATGATGCAGCGACCGG
  AGTCCACTGCAGCTGTCCTGTGGGTTTCATGCTGCAGCCAGACAGAAAGACCTGCAAAGATATAGACGAG
  TGCCGCTTAAACAACGGGGGCTGTGACCACATCTGCCGCAACACAGTGGGCAGCTTCGAGTGCAGCTGCA
  AGAAGGGCTACAAGCTTCTCATCAACGAAAGGAGCTGCCAGGATATAGATGAGTGCTCCTTCGACCGAAC
  CTGTGACCACATGTGTGTCAACACCCCGGGGAGCTTCCAGTGTCTCTGTCATCGTGGCTACCTTCTATAT
  GGTGTCACTCACTGCGGGGATGTGGACGAATGTAGCATCAATAAGGGAGGCTGCCGCTTTGGCTGCATCA
  ACACCCCAGGCAGCTATCAGTGTACCTGCCCAGCAGGCCAGGGCCGCCTTCACTGGAACGGGAAGGATTG
  CACAGAGCCAGTGAAGTGTCAGAGCAGTCTTGGAGCCTCAAAAGCCATGCTTAGCTGCAATCGCTCGGGC
  AAGAAGGATACTTGTGCCCTGACCTGTCCCTCCCGGGCCCGGTTCTTGCCAGAGTCAGAGAACGGTTTCA
  CCGTGAGCTGTGGTACCCCGAGCCCTAAAGCTGCTCCAGCCCGAGTGATCCACTCTGGGAACAGCACCGT
  CTCCAGCTCCTGCCATGAGGCTGCAGTGCTGCCAGTCAAGCAGAGAGCCTCCTTCAAGATCAAGGATGCC
  AAATGTCGCCTGCATCTGCGAAACAAAGGCAAAGCCGAGGAGGCCAGCAGGATCCTGGGGCCAGGTAGTG
  TCCCCTGCTCTGACTGCCTGGTCACTTTCATCCACCTTAAGTGTGACTCTTCTCGGAAGGGCAAGGGCAG
  GCGGGCCCGGACCCCCCCAGGCAAGGAGGTTACCCGGCTCACCCTGGAATTGGAAGCTGAGGTCAGAGCA
  GAAGAAACGACAGCTGGCTGTGGGCTGCCTTGCCTCCGACAGCGGATGGAACGGAGGCTGAAAGGGTCCC
  TGAAGATGCTCAGAAAGTCCATCAACCAAGACCGGTTCCTGCTGCGTCTGGCAGGCCTTGACTATGAGCT
  AGCCCACAAGCCAGGCCTGGGAGCTGGGGACCGAGCAGAGCTGGTGGAGGTCTGCCGACCTGGGCAGCAC
  CGAGCAGGCACCAAGTGTGTCAGCTGCCCGCAGGGAACGTATTACCACGGCCAGACGGAGCAGTGTGTGC
  CATGCCCAGCGGGCACCTTCCAGGAGAGGGAAGGGCAGCTCTCCTGCGACCTTTGCCCTGGGAGTGATGC
  CCATGGGCCTCTTGGAGCCACCAACGTCACCACGTGTGCAGGTCAGTGCCCACCTGGCCACCATTCAGGA
  GATGGGTTTAAGCCCTGCCAGCCATGCCCCCGGGGCACCTATCAGCCTGAAGCAGGGCGAACCCTGTGCT
  TCCCCTGTGGCGGAGGCCTCACCACCAAGCATGAGGGGGCAGTCTCCTTCCAGGACTGCGACACCAAAGT
  CCAGTGCTCCCCGGGCCACTACTATAACACTAGCATCCATCGCTGTATCCGCTGTGCTGTGGGCTCCTAC
  CAGCCAGATTTCCGTCAGAACTTCTGCACCCGCTGCCCAGGAAATACAAGCACTGACTTTGATGGCTCGA
  CCAGTGTGGCCCAGTGCAAGAATCGGCAGTGTGGCGGGGAGCTAGGTGAGTTCACTGGCTACATCGAGTC
  TCCCAACTACCCGGGCAACTACCCGGCCGGTGTGGAGTGCGTCTGGAACATCAACCCCCCACCCAAGCGC
  AAGATCCTCATCGTGGTCCCCGAGATCTTCCTGCCCTCCGAGGATGAGTGTGGGGACGTCCTGGTCATGA
  GAAAGAACTCCTCCCCATCCTCTATCACCACTTACGAGACCTGCCAGACCTACGAGCGGCCCATTGCCTT
  CACCGCCCGGTCCAGGAAACTCTGGATCAACTTCAAGACCAGTGAGGCCAACAGCGCTCGAGGCTTCCAG
  ATTCCCTATGTGACCTACGATGAGGACTATGAGCAGCTGGTGGAAGACATAGTGCGTGATGGCCGGCTGT
  ATGCCTCAGAAAACCACCAGGAGATCCTAAAGGACAAGAAGCTTATCAAGGCCTTCTTTGAGGTCCTGGC
  CCACCCTCAAAACTACTTCAAGTACACAGAGAAACACAAGGAGATGCTGCCAAAATCCTTCATCAAGCTA
  CTGCGCTCCAAAGTCTCCAGCTTCCTGAGGCCTTACAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225155 representing NM_001004366
  Red=Cloning site Green=Tags(s)
  
  MGSGRVPGLCLLLLLVHARAAQHGKAAQDVDECVEGTDNCHIDAICQNTPRSYKCICKSGYTGDGKHCKD
  VDECEREDNAGCVHDCVNIPGNYRCTCYDGFHLAHDGHNCLDVDECAEGNGGCQQSCVNMMGSYECHCRD
  GFFLSDNQHTCIQRPEEGMNCMNKNHGCAHICRETPKGGIACECRPGFELTKNQRDCKLTCNYGNGGCQH
  TCDDTEQGPRCGCHVKFVLHTDGKTCIETCAVNNGGCDSKCHDAATGVHCSCPVGFMLQPDRKTCKDIDE
  CRLNNGGCDHICRNTVGSFECSCKKGYKLLINERSCQDIDECSFDRTCDHMCVNTPGSFQCLCHRGYLLY
  GVTHCGDVDECSINKGGCRFGCINTPGSYQCTCPAGQGRLHWNGKDCTEPVKCQSSLGASKAMLSCNRSG
  KKDTCALTCPSRARFLPESENGFTVSCGTPSPKAAPARVIHSGNSTVSSSCHEAAVLPVKQRASFKIKDA
  KCRLHLRNKGKAEEASRILGPGSVPCSDCLVTFIHLKCDSSRKGKGRRARTPPGKEVTRLTLELEAEVRA
  EETTAGCGLPCLRQRMERRLKGSLKMLRKSINQDRFLLRLAGLDYELAHKPGLGAGDRAELVEVCRPGQH
  RAGTKCVSCPQGTYYHGQTEQCVPCPAGTFQEREGQLSCDLCPGSDAHGPLGATNVTTCAGQCPPGHHSG
  DGFKPCQPCPRGTYQPEAGRTLCFPCGGGLTTKHEGAVSFQDCDTKVQCSPGHYYNTSIHRCIRCAVGSY
  QPDFRQNFCTRCPGNTSTDFDGSTSVAQCKNRQCGGELGEFTGYIESPNYPGNYPAGVECVWNINPPPKR
  KILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQ
  IPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQNYFKYTEKHKEMLPKSFIKL
  LRSKVSSFLRPYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004366
• ORF Size: 2979 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004366.1, NP_001004366.1
• RefSeq Size: 2982 bp
• RefSeq ORF: 2982 bp
• Locus ID: 268935
• Cytogenetics: 17 A3.3