Pde5a (NM_153422) Mouse Tagged ORF Clone

CAT#: MG225179

Pde5a (GFP-tagged) - Mouse phosphodiesterase 5A cGMP-specific (Pde5a), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde5a
• Synonyms: Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225179 representing NM_153422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGAGCGGGCCCCAACTCCGTGCGGTCGCAGCAGCAGCGGGACCCGGACTGGGTGGAAGCGTGGC
  TGGATGATCACCGGGACTTTACCTTCTCTTACTTTATTAGAAAGGCCACCAGAGACATGGTCAACGCATG
  GTTTTCAGAGAGAGTTCACAACATCCCTGTGTGCAAGGAAGGCATCCGAGCCCATACCGAGTCCTGCTCT
  TGCTCTCTGCAGCAAAGTCCTCATGCAGATAATACCACCCCTGGAGCACCAGCCAGAAAAATATCTGCCT
  CTGAATTTGACCGACCTCTTAGACCCATTGTTGTCAAGGATTCCGAGGGAACAGTGAGCTTTCTCTCTGA
  CTCAGGAAAAAAGGAACAAATGCCACTAACCCCCCCAAGATTTGATAGTGATGAAGGGGACCAGTGCTCA
  AGGCTCTTGGAGCTAGTAAAAGATATTTCCAGTCATTTGGATGTCACAGCATTGTGTCACAAAATTTTCT
  TGCATATCCATGGACTCATCTCTGCGGATCGCTATTCCCTGTTCCTTGTCTGTGAGGACAGCTCTAAAGA
  CAAATTCCTCATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACATTGGAGGAAGCTTCAAATAACTGT
  ATCCGTTTAGAGTGGAACAAAGGCATTGTGGGACATGTGGCAGCTTTTGGCGAGCCCTTGAACATCAAAG
  ATGCCTATGAGGATCCCCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCT
  TTGTATGCCAATTAAGAACCACAGGGAAGAGGTTGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGA
  AATGGTGGGACCTTCACTGAAAAAGATGAAAAGGACTTTGCGGCCTACCTGGCATTCTGTGGTATTGTTC
  TTCACAATGCTCAGCTCTATGAAACGTCATTGCTGGAGAACAAAAGAAATCAGGTGTTACTTGACCTTGC
  CAGCTTAATCTTTGAAGAGCAGCAGTCACTGGAAGTCATTCTGAAGAAGATAGCGGCCACTATCATCTCC
  TTCATGCAGGTGCAGAAGTGCACCATCTTCATTGTGGATGAAGACTGTCCTGACTCTTTCTCTCGTGTGT
  TTCACATGGAGTGTGAGGAAGTAGGAAAACCCTCTGATCCTTTAACGAGGGAGCAAGATGCAAACAAAAT
  CAACTACATGTATGCTCAGTATGTCAAAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGAC
  AAAAGATTTCCCTGGACAAATGAAAACATGGGACACGTGAACACGCCCTGCATTGGAAGTTTGCTTTGCA
  CCCCTATAAAAAATGGGAAGAAGAACAAAGTCATAGGCGTCTGCCAACTTGTTAATAAGATGGAGGAGAA
  TACTGGCAAGATTAAAGCTTTCAACCAAAATGATGAGCAGTTCCTGGAAGCCTTTGTCATCTTCTGTGGC
  TTGGGGATCCAGAACACACAGATGTATGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACATTAG
  AGGTCCTGTCTTACCATGCATCAGCGGCGGAGGAAGAAACAAGAGAGCTCCAGGCTTTATCGGCTGCTGT
  GGTACCATCGGCCCAAACCCTTAAAATTACCGACTTCAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAA
  ACAGCGCTGTGTACAATTCGGATGTTCACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGG
  TTCTTTGCAGGTGGATTTTAAGTGTGAAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCA
  TGCCTTTAACACGGCACAGTGCATGTTTGCTGCTCTGAAAGCCGGCAAGATCCAGAACAAGTTGACGGAT
  CTGGAGACCCTTGCGTTGCTCATTGCTGCTTTAAGCCATGACTTGGATCATCGTGGCGTGAACAACTCAT
  ACATACAGCGGAGCGAGCACCCGCTGGCTCAGCTGTACTGTCACTCCATCATGGAACACCATCATTTTGA
  CCAGTGCTTGATGATTCTAAACAGCCCAGGCAACCAGATTCTCAGTGGCCTCTCCATTGACGAATATAAG
  ACCACACTGAAAATAATCAAGCAAGCAATTTTAGCCACAGACCTAGCACTGTACATTAAGAGACGAGGAG
  AATTTTTTGAACTTATACGAAAAAATCAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCTAGC
  AATGCTGATGACAGCCTGCGACCTCTCTGCGATTACTAAACCCTGGCCTATTCAACAACGGATAGCAGAA
  CTCGTGGCAGCTGAATTCTTTGATCAAGGAGACAGAGAGAGAAAAGAACTTAACATGGAGCCGGCTGATC
  TAATGAACAGAGAGAAGAAAAATAAAATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCA
  GCTGTATGAGGCCCTGACCCACGTATCCGAAGACTGTTTGCCCTTGCTGGATGGCTGCAGGAAGAACAGA
  CAGAAATGGCAGGCCCTCGCGGAGCAGCAGGAGAAGATGCTCCTTAACGGGGAGAGCAGCCAGGGCAAGC
  GGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225179 representing NM_153422
  Red=Cloning site Green=Tags(s)
  
  MERAGPNSVRSQQQRDPDWVEAWLDDHRDFTFSYFIRKATRDMVNAWFSERVHNIPVCKEGIRAHTESCS
  CSLQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGDQCS
  RLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNC
  IRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSG
  NGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIIS
  FMQVQKCTIFIVDEDCPDSFSRVFHMECEEVGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKD
  KRFPWTNENMGHVNTPCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKAFNQNDEQFLEAFVIFCG
  LGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALSAAVVPSAQTLKITDFSFSDFELSDLE
  TALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTD
  LETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIDEYK
  TTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAE
  LVAAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNR
  QKWQALAEQQEKMLLNGESSQGKRD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153422
• ORF Size: 2595 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153422.2, NP_700471.2
• RefSeq Size: 6728
• RefSeq ORF: 2598
• Locus ID: 242202