Lrrk2 (NM_025730) Mouse Tagged ORF Clone

CAT#: MG225348

Lrrk2 (GFP-tagged) - Mouse leucine-rich repeat kinase 2 (Lrrk2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lrrk2
• Synonyms: 4921513O20Rik; 9330188B09Rik; AW561911; cI-46; D630001M17Rik; Gm927
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225348 representing NM_025730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTGGCGCCTGTCAGGGCTGCGAAGAGGAAGAGGAGGAGGAGGCTCTGAAGAAGTTGATAGTCA
  GGCTGAATAATGTCCAGGAAGGCAAGCAGATCGAGACGTTGCTTCAGCTCCTGGAGGACATGCTGGTGTT
  CACCTACTCGGACCGCGCCTCCAAGTTATTTGAAGATAAAAATTTCCACGTGCCTCTGTTGATTGTCCTG
  GACTCCTACATGAGAGTTGCCAGTGTACAGCAGGCGGGGTGGTCACTTCTGTGCAAATTAATAGAAGTCT
  GTCCAGGGACATTGCAAAGCTTAATAGGACCCCAGGATATTGGAAATGATTGGGAAGTCCTTGGTATTCA
  CCGGCTGATTCTTAAAATGTTAACTGTTCATCACGCCAATGTAAACCTGTCAATAGTTGGACTAAAAGCC
  TTGGATCTCCTCCTAGATTCAGGTAAACTCACCTTGCTGATACTGGATGAAGAATGTGATATTTTCTTGT
  TAATTTTTGATGCCATGCACAGATATTCAGCCAATGATGAAGTCCAAAAACTGGGATGCAAAGCTTTACA
  CGTGCTTTTTGAGAGAGTTTCCGAGGAACAGCTGACTGAGTTTGTGGAGAACAAAGATTACACGATACTG
  CTGAGTACGTTCGGCAGCTTCAGAAGGGACAAGGAGATTGTGTACCACGTACTTTGCTGCTTGCATTCCC
  TGGCGGTTACATGCAGCAATGTAGAGGTCCTCATGAGTGGGAATGTCCGGTGCTACAATCTTGTGGTGGA
  GGCCATGAAAGCCTTCCCCACCAATGAAAACATCCAAGAGGTGAGCTGCTCCTTGTTCCAGAAGCTTACA
  TTAGGTAACTTTTTCAACATCCTGGTGTTGAACGAAGTGCATGTCTTTGTGGTGAAAGCGGTCCGACAGT
  ATCCTGAGAACGCAGCCTTACAGATCTCTGCACTCAGCTGTTTAGCACTCCTCACTGAGACTATTTTCTT
  AAACCAAGACTTGGAGGAAAGAAGTGAGACTCAAGAGCAGAGCGAAGAGGAAGACAGTGAGAAGCTTTTC
  TGGCTGGAACCCTGCTATAAAGCCCTGGTGCGCCATCGAAAGGACAAACACGTGCAGGAGGCTGCCTGCT
  GGGCACTAAATAACCTCCTTATGTACCAGAACAGTTTGCATGAGAAGATCGGAGATGAAGATGGCCAGTT
  CCCTGCGCACAGGGAAGTGATGCTGTCTATGCTGATGCACTCTTCTTCCAAAGATGTCTTCCAAGCAGCT
  GCACATGCTCTGTCCACTCTCTTGGAACAAAATGTTAATTTCAGGAAAATCCTGCTGGCAAAAGGAGTAT
  ACCTGAATGTCTTGGAATTGATGCAGAAGCATGCCCATGCGCCTGAGGTGGCAGAGAGTGGCTGCAAGAT
  GCTGAGTCACCTGTTTGAAGGAAGTAACCCTTCTCTGGATACAATGGCAGCAGTGGTCCCTAAAATACTA
  ACAGTGATGAAAGCCCACGGAACGTCTCTGTCAGTCCAGCTGGAGGCGCTGCGAGCTATCTTGCATTTCG
  TTGTGCCAGGACTATTGGAAGAATCCAGGGAGGACTCTCAATGCAGACCAAATGTGCTCAGAAAACAGTG
  TTTCAGGACTGACATCCACAAGCTGGTTCTAGTCGCTCTGAACAGGTTCATTGGGAATCCTGGGATTCAG
  AAATGTGGATTGAAAGTAATCTCTTCTCTCGCGCACCTTCCTGATGCCACAGAGACATTGTCCCTGCAAG
  GAGCAGTTGACTCAGTCCTCCACACCTTACAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGCTT
  ACACCTTATGGGATGCTTGATGACAAAGAAGAATTTCTGCATAGGGACAGGGCACCTCCTGGCAAAAATT
  CTGGCTTCCACTTTGCAGCGCTTTAAAGATGTTGCTGAGGTGCAGACTACAGGATTACAGACAACCCTGT
  CAATACTTGAGCTGTCAGTATCTTTCTCCAAGCTGCTAGTGCACTATTCCTTTGATGTGGTGATATTTCA
  TCAGATGTCTTCCAGTGTTGTAGAACAAAAGGATGAGCAGTTCCTCAATCTATGTTGCAAATGCTTTGCA
  AAAGTGGCCGTGGATGATGAGCTGAAAAACACCATGCTAGAGAGAGCCTGCGATCAGAATAACAGCATCA
  TGGTTGAATGTTTGCTCCTCTTGGGAGCTGATGCCAACCAAGTGAAGGGGGCAACTTCTTTAATCTATCA
  GGTATGTGAGAAAGAGAGCAGTCCTAAATTGGTGGAACTGTTGCTTAATGGTGGTTGTCGTGAACAAGAT
  GTACGGAAGGCCCTGACCGTAAGCATCCAAAAGGGCGACAGCCAGGTCATCAGCTTGCTCCTCAGGAAAC
  TTGCCCTGGACCTGGCCAACAACAGCATTTGCCTTGGAGGATTTGGCATAGGAAAAATTGATCCTTCTTG
  GCTTGGTCCTTTATTTCCAGATAAGTCATCCAATTTAAGGAAGCAAACAAACACAGGATCTGTCCTAGCG
  AGGAAAGTGCTCCGGTATCAGATGAGAAACACCCTTCAAGAAGGCGTGGCCTCAGGCAGTGACGGCAAGT
  TTTCTGAAGACGCGCTGGCGAAATTTGGAGAATGGACCTTTATTCCCGACTCTTCTATGGACAGTGTGTT
  TGGCCAGAGCGATGATCTGGATAGCGAAGGCAGCGAGAGCTCATTTCTCGTGAAGAGGAAGTCCAACTCA
  ATTAGTGTAGGGGAAGTTTACAGAGATCTAGCTCTGCAGCGCTGCTCACCAAATGCTCAGAGGCATTCCA
  ATTCGCTGGGTCCTGTTTTTGACCATGAAGACTTACTGAGACGAAAAAGAAAAATACTGTCTTCAGATGA
  GTCTCTCAGGTCCTCAAGGCTGCCGTCCCATATGAGGCAATCAGATAGCTCTTCTTCCCTGGCTTCTGAG
  AGAGAACACATCACGTCGTTAGACCTATCTGCCAACGAACTCAAAGATATTGATGCTCTGAGCCAGAAGT
  GTTGCCTCAGTAGCCACCTGGAACATCTCACCAAACTGGAACTTCACCAGAATTCACTCACGAGCTTCCC
  ACAGCAGCTGTGTGAGACTCTGAAGTGTTTGATACACTTGGATTTGCACAGTAACAAATTCACCTCATTT
  CCCTCTTTCGTGTTGAAAATGCCACGTATCACCAACCTAGATGCCTCTCGAAATGACATCGGGCCAACAG
  TAGTTTTAGACCCTGCGATGAAGTGTCCAAGCCTCAAACAGTTGAATCTGTCCTATAACCAGCTCTCTTC
  AATCCCAGAGAATCTTGCCCAAGTGGTGGAGAAACTTGAGCAGCTCCTACTGGAAGGAAATAAAATATCC
  GGGATTTGCTCTCCCCTGAGCCTGAAGGAACTGAAGATTTTAAATCTTAGTAAAAATCACATTCCATCCC
  TACCTGGAGATTTTCTTGAGGCTTGTTCAAAAGTCGAGAGTTTCAGTGCTCGCATGAATTTTCTTGCTGC
  AATGCCTGCCTTACCTTCTTCCATAACGAGCTTAAAATTGTCTCAGAACTCTTTCACGTGCATTCCAGAA
  GCGATTTTCAGTCTTCCGCACTTGCGGTCCTTGGATATGAGCCACAACAACATTGAATGTCTGCCGGGAC
  CTGCACATTGGAAGTCTCTGAACTTAAGGGAACTCATTTTTAGCAAGAATCAGATCAGCACCTTAGACTT
  TAGTGAGAACCCACACGTGTGGTCAAGAGTAGAGAAACTGCATCTCTCTCATAATAAACTGAAAGAGATT
  CCTCCAGAAATTGGCTGCCTTGAAAATCTGACGTCTCTCGACGTCAGTTACAACTTGGAACTGAGGTCCT
  TCCCAAATGAAATGGGGAAGTTGAGCAAGATATGGGATCTTCCCTTGGACGGACTGCATCTGAATTTTGA
  CTTTAAGCACGTAGGATGCAAGGCCAAAGACATCATAAGGTTTCTACAACAACGTCTGAAAAAGGCTGTA
  CCCTACAACCGAATGAAGCTCATGATTGTGGGAAATACGGGGAGCGGTAAGACCACTTTACTGCAACAAC
  TCATGAAAATGAAGAAACCAGAACTTGGCATGCAGGGTGCCACAGTCGGCATAGACGTGCGAGACTGGTC
  CATCCAAATACGGGGCAAAAGGAGAAAGGACCTGGTTCTAAACGTGTGGGATTTTGCAGGTCGTGAGGAA
  TTCTACAGCACTCACCCCCACTTCATGACCCAGAGAGCCCTCTACCTGGCTGTCTATGATCTCAGCAAGG
  GGCAGGCAGAGGTGGACGCCATGAAGCCCTGGCTCTTCAATATCAAGGCTCGTGCCTCTTCTTCCCCGGT
  GATTCTGGTGGGCACACATTTGGATGTTTCTGATGAGAAGCAGCGGAAAGCGTGCATAAGCAAAATCACG
  AAGGAACTCCTAAATAAGCGAGGATTCCCCACCATCCGGGACTACCACTTTGTGAATGCCACCGAGGAGT
  CAGATGCGCTGGCAAAGCTTCGGAAAACCATCATAAATGAGAGCCTTAATTTCAAGATCCGAGATCAGCC
  TGTGGTTGGGCAGCTAATTCCAGATTGCTACGTAGAACTGGAGAAAATCATTTTATCAGAGCGGAAAGCT
  GTGCCGACTGAGTTTCCTGTGATTAACCGGAAACACCTGTTACAGCTCGTGAACGAACATCAGCTGCAGC
  TGGATGAGAACGAGCTCCCACACGCCGTTCACTTCCTAAATGAGTCGGGAGTTCTTCTGCATTTTCAAGA
  CCCTGCCCTGCAGCTAAGTGACCTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAGTCATGGCACAGATC
  TTGACAGTGAAGGTAGACGGCTGTCTGAAACATCCTAAGGGCATCATTTCCCGGAGAGATGTGGAAAAAT
  TCCTTTCAAAGAAGAAGCGATTCCCGAAGAACTATATGATGCAATACTTTAAACTATTAGAAAAATTTCA
  GATCGCATTGCCAATAGGGGAAGAATATCTTCTGGTTCCAAGCAGCTTGTCTGACCACAGGCCAGTGATA
  GAGCTCCCCCACTGTGAGAACTCTGAGATCATCATCCGGCTGTACGAAATGCCGTACTTTCCCATGGGAT
  TTTGGTCAAGATTGATTAACCGATTACTTGAAATCTCACCCTTCATGCTTTCTGGCAGAGAGAGAGCACT
  ACGCCCTAACAGGATGTATTGGCGGCAAGGCATCTACTTGAATTGGTCTCCAGAAGCATACTGTCTGGTA
  GGCTCTGAAGTCTTAGACAATCGACCTGAGAGTTTCTTGAAAATCACAGTTCCGTCTTGTAGAAAAGGTT
  GTATTCTTCTGGGCCGAGTTGTGGATCATATTGACTCACTCATGGAAGAATGGTTTCCCGGGTTACTGGA
  GATTGACATTTGTGGGGAAGGAGAAACTCTGTTGAAGAAATGGGCATTGTACAGTTTTAATGATGGTGAA
  GAACATCAGAAGATCTTGCTTGATGAGTTGATGAAGAAGGCTGAAGAAGGAGACCTGTTAATAAATCCAG
  ACCAACCAAGGCTCACTATTCCAATATCCCAGATTGCTCCGGACTTGATCTTGGCTGACCTGCCTAGAAA
  TATCATGTTGAACAATGATGAGTTGGAATTTGAGGAAGCACCAGAGTTTCTCTTAGGCGATGGAAGTTTT
  GGATCCGTTTATCGAGCTGCCTACGAAGGAGAGGAAGTGGCTGTGAAGATTTTTAATAAGCACACATCTC
  TTAGGCTGTTAAGACAAGAGTTGGTGGTCCTTTGTCACCTTCACCACCCCAGCCTGATATCCTTGCTGGC
  GGCTGGTATTCGTCCTCGGATGTTGGTAATGGAGTTGGCCTCCAAAGGTTCCTTGGATCGCCTGCTGCAG
  CAGGACAAAGCCAGCCTCACCAGAACCCTCCAGCACAGGATCGCGTTGCATGTGGCCGACGGCCTGAGGT
  ATCTCCACTCAGCCATGATTATTTACCGTGACCTGAAGCCCCACAATGTGCTGCTTTTTACCCTGTATCC
  CAATGCTGCCATCATTGCGAAGATTGCGGACTACGGGATCGCACAGTACTGCTGCAGGATGGGAATAAAG
  ACATCAGAGGGCACCCCAGGGTTCCGGGCACCTGAAGTTGCCAGGGGGAATGTCATTTATAACCAACAGG
  CCGATGTTTATTCTTTTGGCTTACTACTTCACGATATTTGGACAACTGGGAGTAGGATTATGGAGGGTTT
  GAGGTTCCCAAATGAGTTTGATGAGTTAGCCATACAAGGGAAGTTGCCAGATCCAGTTAAAGAATATGGC
  TGTGCCCCATGGCCTATGGTTGAGAAGTTAATTACAAAGTGTTTGAAAGAAAATCCTCAAGAAAGACCCA
  CTTCTGCCCAGGTCTTTGACATTTTGAATTCGGCTGAATTAATTTGCCTCATGCGACACATTTTAATACC
  TAAGAACATCATTGTTGAATGCATGGTTGCCACGAATCTCAATAGCAAGAGTGCGACTCTCTGGTTGGGA
  TGTGGGAACACAGAAAAAGGACAGCTTTCCTTATTTGACTTAAACACGGAAAGATACAGCTATGAGGAAG
  TTGCTGATAGTAGAATACTGTGCTTGGCTTTGGTGCATCTCGCTGCTGAGAAAGAGAGCTGGGTTGTGTG
  CGGGACACAGTCTGGGGCTCTCCTGGTCATCAATGTTGAAGAGGAGACAAAGAGACACACCCTGGAAAAG
  ATGACTGATTCTGTCACTTGTTTGCATTGCAATTCCCTTGCCAAGCAGAGCAAGCAAAGTAACTTTCTTT
  TGGTGGGAACTGCTGATGGTAACTTAATGATATTTGAAGATAAAGCCGTTAAGTGTAAAGGAGCTGCCCC
  CTTGAAGACACTACACATAGGCGATGTCAGTACGCCCCTGATGTGCCTGAGCGAGTCCCTGAATTCATCT
  GAAAGACACATCACATGGGGAGGGTGTGGCACAAAGGTCTTCTCCTTTTCCAATGATTTCACCATTCAGA
  AACTCATCGAGACAAAAACCAACCAGCTGTTTTCTTACGCAGCTTTCAGCGATTCTAACATCATAGCGCT
  GGCAGTAGACACAGCCCTGTATATTGCCAAGAAAAACAGCCCTGTCGTAGAGGTGTGGGACAAGAAAACA
  GAAAAGCTCTGTGAATTAATAGACTGTGTGCACTTCTTAAAGGAGGTGATGGTAAAACTAAACAAGGAAT
  CGAAACATCAGCTGTCCTACTCTGGGAGGGTGAAGGCCCTCTGCCTGCAGAAGAACACGGCTCTCTGGAT
  CGGAACTGGAGGAGGCCACATCTTACTCCTGGATCTTTCTACTCGGCGAGTTATCCGCACCATTCACAAT
  TTCTGTGATTCTGTGAGAGCCATGGCCACAGCACAATTAGGAAGCCTTAAGAATGTCATGCTGGTTTTGG
  GGTACAAGCGGAAGAGTACAGAGGGTATCCAAGAACAAAAAGAGATACAATCTTGTTTGTCTATTTGGGA
  CCTCAATCTTCCACACGAGGTGCAAAATTTAGAAAAACACATTGAAGTAAGAACAGAATTAGCTGATAAA
  ATGAGGAAAACATCTGTTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225348 representing NM_025730
  Red=Cloning site Green=Tags(s)
  
  MASGACQGCEEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVL
  DSYMRVASVQQAGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKA
  LDLLLDSGKLTLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTIL
  LSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENIQEVSCSLFQKLT
  LGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFLNQDLEERSETQEQSEEEDSEKLF
  WLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAA
  AHALSTLLEQNVNFRKILLAKGVYLNVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKIL
  TVMKAHGTSLSVQLEALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQ
  KCGLKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKI
  LASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSSSVVEQKDEQFLNLCCKCFA
  KVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKGATSLIYQVCEKESSPKLVELLLNGGCREQD
  VRKALTVSIQKGDSQVISLLLRKLALDLANNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLA
  RKVLRYQMRNTLQEGVASGSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNS
  ISVGEVYRDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSSLASE
  REHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLIHLDLHSNKFTSF
  PSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQLSSIPENLAQVVEKLEQLLLEGNKIS
  GICSPLSLKELKILNLSKNHIPSLPGDFLEACSKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPE
  AIFSLPHLRSLDMSHNNIECLPGPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEI
  PPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAV
  PYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLVLNVWDFAGREE
  FYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACISKIT
  KELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKA
  VPTEFPVINRKHLLQLVNEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQI
  LTVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVI
  ELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLV
  GSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGE
  EHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSF
  GSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQ
  QDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIK
  TSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYG
  CAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATLWLG
  CGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEK
  MTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSS
  ERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKT
  EKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHN
  FCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADK
  MRKTSVE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025730
• ORF Size: 7581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025730.3, NP_080006.3
• RefSeq Size: 8231
• RefSeq ORF: 7584
• Locus ID: 66725