En1 (NM_010133) Mouse Tagged ORF Clone

CAT#: MG225925

En1 (GFP-tagged) - Mouse engrailed 1 (En1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: En1
• Synonyms: En-1; engrailed-1; Mo-en.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225925 representing NM_010133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAACAGCAGCCGGAGCCTAAAAGTCAGCGCGACTCGGGCCTCGGCGCGGTGGCAGCGGCGGCCC
  CGAGCGGCCTCAGTCTGAGTCTGAGCCCAGGAGCCAGCGGCAGCAGCGGCAGCGATGGAGACAGCGTGCC
  GGTGTCCCCGCAGCCAGCGCCCCCGTCGCCTCCTGCGGCACCCTGTCTGCCGCCCCTGGCCCATCACCCG
  CACCTCCCCCCGCATCCCCCGCCCCCGCCGCCGCCGCCGCCGCCGCCACCGCAGCATCTCGCGGCGCCTG
  CTCACCAGCCGCAGCCCGCGGCCCAGCTGCACCGCACCACCAACTTTTTCATCGATAACATCCTAAGGCC
  CGATTTCGGTTGCAAAAAGGAACAGCCCCTGCCTCAGCTCCTGGTGGCTTCGGCTGCAGCCGGAGGAGGC
  GCAGCAGCAGGAGGAGGAAGCCGCGTGGAGCGTGACCGAGGCCAGACTGGTGCAGGTAGAGACCCCGTTC
  ACTCTCTGGGCACACGAGCTTCGGGGGCTGCCTCGCTCTTGTGTGCTCCAGATGCGAACTGTGGCCCACC
  CGACGGCTCCCAGCCCGCCACCGCTGTCAGCGCCGGCGCATCCAAAGCCGGGAACCCGGCTGCTGCGGCG
  GCCGCGGCCGCAGCAGCGGCTGCAGCGGCAGTGGCGGCAGCGGCGGCAGCAGCCTCGAAGCCCTCGGACA
  GTGGCGGTGGTAGTGGAGGCAACGCGGGGAGTCCCGGGGCGCAGGGCGCCAAGTTCCCGGAACACAACCC
  TGCGATCCTACTCATGGGTTCGGCTAACGGTGGGCCGGTGGTCAAGACTGACTCACAGCAACCCCTAGTG
  TGGCCCGCCTGGGTCTACTGCACACGCTATTCGGACCGTCCGTCCTCTGGTCCACGCACCAGGAAGCTAA
  AGAAGAAAAAGAACGAGAAGGAAGACAAGCGGCCGCGGACGGCGTTCACGGCCGAGCAGCTGCAGAGACT
  CAAGGCGGAGTTCCAGGCAAACCGCTATATCACGGAGCAGCGGCGACAGACCCTCGCCCAGGAGCTCAGC
  CTGAATGAGTCCCAGATCAAGATCTGGTTCCAAAACAAGCGTGCCAAGATCAAGAAAGCCACCGGCATCA
  AGAACGGCCTGGCGCTGCACCTCATGGCCCAGGGACTGTACAACCACTCTACCACCACGGTTCAGGACAA
  AGACGAGAGCGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225925 representing NM_010133
  Red=Cloning site Green=Tags(s)
  
  MEEQQPEPKSQRDSGLGAVAAAAPSGLSLSLSPGASGSSGSDGDSVPVSPQPAPPSPPAAPCLPPLAHHP
  HLPPHPPPPPPPPPPPPQHLAAPAHQPQPAAQLHRTTNFFIDNILRPDFGCKKEQPLPQLLVASAAAGGG
  AAAGGGSRVERDRGQTGAGRDPVHSLGTRASGAASLLCAPDANCGPPDGSQPATAVSAGASKAGNPAAAA
  AAAAAAAAAAVAAAAAAASKPSDSGGGSGGNAGSPGAQGAKFPEHNPAILLMGSANGGPVVKTDSQQPLV
  WPAWVYCTRYSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELS
  LNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDESE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010133
• ORF Size: 1203 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010133.2, NP_034263.2
• RefSeq Size: 2554
• RefSeq ORF: 1206
• Locus ID: 13798