Magi1 (NM_001029850) Mouse Tagged ORF Clone

CAT#: MG226035

Magi1 (GFP-tagged) - Mouse membrane associated guanylate kinase WW and PDZ domain containing 1 (Magi1) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Magi1
• Synonyms: AIP3; Baiap1; BAP1; Gukmi1; Magi-1; MAGI1c; mKIAA4129; TNRC19; WWP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226035 representing NM_001029850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAAGTGATCCAGAAGAAGAACCACTGGACTGGCCGCGTTCACGAGTGCACCGTGAAGCGGGGAC
  CCCAGGGCGAGCTGGGGGTGACGGTCCTGGGGGGCGCGGAGCATGGGGAGTTTCCGTACGTGGGGGCGGT
  GGCGGCGGCCGAGGCGGCGGGGCTTCCCGGCGGTGGCGAGGGGCCGAAGCTGGCCGAAGGTGAGCTGCTG
  CTGGAGGTGCAGGGGGTCCGGGTGTCCGGCTTGCCCCGCTATGACGTGCTGGGAGTCATCGACAGCTGCA
  AGGAGGCCGTCACCTTCAAAGCCGTCAGACAAGGAGGAAGGCTCAACAAGGACCTACGACATTTCCTCAA
  CCAACGGTTCCAGAAGGGGTCTCCAGATCATGAGCTCCAGCAGACCATAAGGGACAACCTCTACCGCCAT
  GCTGTGCCTTGCACAACCCGGTCTCCCAGAGAAGGAGAAGTGCCTGGTGTGGATTACAGCTTTCTGACTG
  TGAAGGAGTTCTTGGACCTCGAGCAGAGCGGGACCCTGTTGGAAGTCGGCACCTATGAAGGAAACTATTA
  TGGGACACCCAAACCTCCTAGCCAGCCAGTCAGTGGGAAAGTGATCACGACGGATGCCTTGCACAGCCTG
  CAGTCTGGCTCCAAGCAGTCGACCCCTAAGCGAACAAAGTCCTACAATGATATGCAAAATGCTGGCATAG
  TCCACCCGGAGAATGAGGAGGAGGAGGATGTCCCTGAAATGAACAGTAGCTTTACAGCCGACTCTGGAGA
  CCAGGACGAGCACACTCTCCAAGAAGCAACGCTCCCGCCTGTGAATAGTAGCATCCTCGCTGCTCCCATC
  ACGGACCCTTCTCAGAAGTTCCCTCAGTACCTACCTCTTTCTGCAGAGGATAATTTAGGTCCTCTACCTG
  AAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTCATAGACCACAACACGAAAACAACATC
  ATGGTTAGACCCTCGGTGCCTGAACAAACAGCAGAAGCCTCTGGAAGAATGTGAAGATGATGAAGGGGTA
  CACACCGAGGAGCTGGACAGTGAACTAGAGTTGCCTGCTGGCTGGGAAAAGATTGAAGACCCTGTCTACG
  GTGTCTACTATGTAGACCACATCAACAGGAAGACGCAATATGAAAACCCAGTCCTAGAAGCCAAACGGAA
  GAAACAGCTTGAACAGCAGCAGCAACAGCAGCAGCCTCAGCCACCGCAGCCAGAAGAGTGGACAGAGGAT
  CATGCATCTGTTGTGCCTCCTGTTGCTCCTTCCCATCCCCCGAGCAATCCGGAGCCAGCCAGGGAAACTC
  CACTTCAGGGCAAACCTTTTTTTACAAGAAACCCCTCTGAGCTGAAAGGCAAGTTCATTCACACGAAGCT
  ACGGAAAAGCAGCCGAGGCTTTGGCTTCACGGTGGTTGGAGGAGACGAGCCTGATGAGTTCCTGCAGATC
  AAGAGCCTCGTCCTCGATGGTCCTGCCGCACTGGATGGCAAGATGGAGACAGGGGATGTAATTGTGAGTG
  TGAATGACACCTGTGTTTTGGGACACACACATGCTCAAGTTGTGAAAATCTTCCAGTCCATTCCCATTGG
  TGCCAGTGTGGACCTTGAACTCTGCAGAGGTTATCCATTGCCTTTTGACCCGGATGACCCTAATACAAGT
  TTAGTGACCTCGGTGGCCATTTTGGACAAAGAACCAATTATTGTAAATGGACAAGAGACCTACGATTCAC
  CAGCGAGCCACAGTAGTAAAACAGGCAAAGTCAGCAGCATGAAGGATGCCAGGCCAAGCAGCCCTGCTGA
  TGTGGCTTCCAACAGCTCTCATGGTTATCCCAACGACACAGTCTCCTTGGCTTCCTCCATAGCCACCCAG
  CCAGAGCTAATAACTGTTCACATAGTCAAAGGGCCAATGGGATTTGGCTTTACGATCGCAGACAGTCCCG
  GTGGGGGTGGCCAAAGAGTGAAACAGATTGTTGACAGTCCACGCTGCAGAGGCCTCAAAGAAGGGGATCT
  TATCGTGGAGGTGAATAAGAAGAACGTGCAGGCCCTGACGCACAATCAAGTCGTGGATATGCTGATTGAA
  TGTCCCAAGGGAAGTGAGGTCACACTGTTGGTGCAGCGAGGAGGGCTACCAGTTCCCAAGAAGAGCCCAA
  AGTCGCAGCCACTGGAGAGGAAAGACAGCCAGAATAGCTCCCAGCACAGCGTCTCCAGCCACCGGAGCCT
  GCACACTGCGTCCCCGAGCCACGGCATACAGGTGCTCCCTGAGTACCTACCTGCAGACGCCCCTGCTCCA
  GATCAGACCGACAGCTCTGGGCAGAAAAAGCCAGATCCTTTTAAAATCTGGGCCCAGTCCAGGAGCATGT
  ATGAAAACCGACCTATGTCACCTTCGCCTGCATCAGGATTGAGCAAGGGTGAAAGAGACAGAGAAATCAA
  TTCCACGAATTTTGGAGAATGTCAGATTCCAGATTACCAGGAACAGGACATCTTCCTCTGGAGAAAAGAA
  ACCGGATTTGGATTTAGGATTCTGGGTGGAAATGAACCAGGGGAACCCATTTATATCGGTCACATCGTAC
  CGCTGGGTGCTGCTGACACAGACGGCCGCCTGAGGTCTGGAGATGAATTAATCTGTGTGGATGGGACACC
  AGTAATTGGGAAATCACACCAGCTCGTGGTCCAGCTTATGCAACAAGCTGCCAAGCAAGGCCATGTCAAT
  CTCACAGTGAGGCGGAAAGTGGTCTTTGCCGTCCCCAAAGCAGAGAATGAGGTGCCCTCACCAGCCTCAT
  CACACCACAGTAGCAACCAGCCCGCGTCCCTGACGGAGGAGAAACGCACACCGCAAGGCAGCCAGAACTC
  TCTGAACACTGTGAGCTCTGGCAGCGGCAGCACCAGTGGCATTGGCAGTGGTGGCGGCGGGGGCAGCGGT
  GTGGTGAGCGCTGTGCTGCAGCCCTATGATGTGGAGATTCGGCGTGGGGAGAACGAGGGCTTTGGGTTTG
  TCATCGTGTCCTCCGTGAGCAGACCCGAAGCGGGCACAACCTTCGGTCGGATTATTGAGGGGAGCCCTGC
  TGACCGCTGTGGCAAGCTGAAAGTAGGAGACCGGATCTTGGCAGTAAATGGATGTTCCATCACCAACAAA
  TCCCATTCTGACATTGTCAACCTAATCAAAGAAGCGGGCAACACAGTGACTCTCCGCATCATCCCCGGGG
  ATGAGTCCTCAAATGCCACGCTGCTGACTAATGCTGAGAAGATTGCCACCATCACCACCACTCATGCCCC
  CTCTCAGCAGGGGACCCAGGAAACAAGGACCACCACCAAACCAAAGCAGGATTCTCAGTTTGAGTTCAAA
  GGACCGCAGGCTGCACAGGAGCAAGATTTCTACACTGTGGAATTGGAAAGAGGGGCCAAGGGATTTGGCT
  TTAGTCTTCGAGGGGGCCGAGAATATAACATGGATCTTTATGTTCTGCGCTTGGCAGAGGATGGTCCTGC
  AGAAAGATGTGGGAAGATGAGGATTGGCGATGAAATTCTAGAGATCAATGGTGAGACCACCAAAAACATG
  AAACACTCTCGGGCCATAGAACTGATCAAGAATGGCGGCCGCAGGGTCCGTCTGTTTCTGCGGCGGGGAG
  ACGGCTCAGTCCCAGAATATGACCCCAGCAGCGACAGGAACGGCCCCTCCACCGGTGCACAAGGTGTTCC
  GGAAGTGAGGCCGGGGCCGCCAGACCACAGACCGCATCCAGCCTTGGAGTCCAGTTACCCACCCGAACTT
  CACAAATCATCACAACATGCCGAAAAGCGAGCACACGCGAAGGATCCAAAAGGCAACAGGGAGCACAGCA
  AACAACCCAACGAACATCACACCTGGAATGGAACTTCTAGAAAACAGGACAGCGGGGCATGCCGGCCCAA
  AGACCGGCCACCCGACGCATGGAGAGAGGCGCAGCCAGAGCGGACAGCCACCAATGGTTCAAAGAGGAGG
  TCACCGGAGAAGCGCAGGGAAGGCACCCGCAGCGCTGACAACACTTTGGAAAGAAGGGAGAAGCATGAGA
  AGAGAAGAGAGATATCTCCCGAGAGGAAGCGAGAGCGTTCACCCACCCGGAGAAAAGATAGCTCCCCGAG
  CCGCCGGCGGAGGTCCCTCGAAAGACTCCTGGATCAAAGACGGTCCCCAGAGCGCAGAAGAGGGGGCTCC
  CCCGAGAGGAGAGCCAAATCCACCGACAGGAGGAGGGCCAGGTCCCCTGAGCGCAGGCGAGAGCGGTCAC
  TGGACAAAAGGAACCGGGACGACAAGGTTGGCCACCGAGAAAGAGAGGAGGCTGGTCTGAAGCTGGAAGC
  GGGGAGAAGCCCCCGAAATCCCCCAGAGCAGAGAAGGCGGCCTTACAAAGAATGTAGCACCGACCTCAGC
  ATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226035 representing NM_001029850
  Red=Cloning site Green=Tags(s)
  
  MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELL
  LEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRH
  AVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSL
  QSGSKQSTPKRTKSYNDMQNAGIVHPENEEEEDVPEMNSSFTADSGDQDEHTLQEATLPPVNSSILAAPI
  TDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGV
  HTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKKQLEQQQQQQQPQPPQPEEWTED
  HASVVPPVAPSHPPSNPEPARETPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQI
  KSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTS
  LVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSSMKDARPSSPADVASNSSHGYPNDTVSLASSIATQ
  PELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQALTHNQVVDMLIE
  CPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSHRSLHTASPSHGIQVLPEYLPADAPAP
  DQTDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQDIFLWRKE
  TGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVN
  LTVRRKVVFAVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSG
  VVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNK
  SHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKQDSQFEFK
  GPQAAQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNM
  KHSRAIELIKNGGRRVRLFLRRGDGSVPEYDPSSDRNGPSTGAQGVPEVRPGPPDHRPHPALESSYPPEL
  HKSSQHAEKRAHAKDPKGNREHSKQPNEHHTWNGTSRKQDSGACRPKDRPPDAWREAQPERTATNGSKRR
  SPEKRREGTRSADNTLERREKHEKRREISPERKRERSPTRRKDSSPSRRRRSLERLLDQRRSPERRRGGS
  PERRAKSTDRRRARSPERRRERSLDKRNRDDKVGHREREEAGLKLEAGRSPRNPPEQRRRPYKECSTDLS
  I
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029850
• ORF Size: 4413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029850.3
• RefSeq Size: 7791 bp
• RefSeq ORF: 4416 bp
• Locus ID: 14924
• Cytogenetics: 6 D1