Gtf2i (NM_010365) Mouse Tagged ORF Clone

CAT#: MG226048

Gtf2i (GFP-tagged) - Mouse general transcription factor II I (Gtf2i) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gtf2i
• Synonyms: 6030441I21Rik; BAP-135; Diws1t; GtfII-I; Spin; TFII-I; WBSCR6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226048 representing NM_010365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAAGTAGTGATGTCTGCCTTGCCTGCCGAAGATGAAGAGTCTTCAGAGAGCAGGATGGTGGTGA
  CCTTCCTCATGTCTGCCCTGGAGTCCATGTGTAAAGAGCTGGCCAAGTCCAAGGCTGAGGTGGCCTGCAT
  TGCTGTGTATGAGACCGACGTGTTTGTTGTTGGAACCGAAAGAGGGCGTGCTTTTGTCAATACCAGAAAA
  GATTTCCAAAAAGACTTTGTGAAGTATTGTGTTGAAGAAGAAGAAAAGGCCGCCGAGATGCATAAGATGA
  AATCTACCACCCAGGCCAATCGGATGAGTGTGGATGCTGTAGAAATTGAAACACTCAGAAAAACGGTGGA
  GGACTATTTCTGTTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTCCCTGTGCCATATGAGAAG
  ATGCTGCGCGACCAGTCGGCTGTGGTGGTGCAGGGGCTTCCCGAGGGGGTTGCCTTCAAGCACCCCGAAC
  ATTACGACCTCGCAACCTTGAAGTGGATTTTGGAGAACAAGGCAGGGATTTCCTTCATCATTAAGAGACC
  TTTCCTCGAGCCGAAGAAACACCTCGGTGGTCGAGTGCTGGCGGCCGAGGCTGAGAGGTCCATGCTGTCT
  CCTAGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCTCTGGAAA
  TGGCCGCTGTGACAGTGAAGGAGGAGTCAGAAGACCCTGATTACTATCAGTATAACATTCAAGGAAGCCA
  TCACTCCTCAGAAGGCAACGAGGGAACGGAAGTGGAAGTGCCAGCAGAAGATTCTACTCAACATGTCCCT
  TCAGAAACCAGTGAGGACCCCGAAGTTGAGGTGACCATTGAAGATGATGACTATTCTCCACCCACCAAGA
  GGCTAAAGAGCACGGAGCCGCCCCCGCCCCCGCCCGTCCCGGAGCCCGCCAACGCTGGCAAGCGCAAAGT
  GAGGGAGTTCAACTTTGAGAAATGGAACGCACGCATCACTGACCTACGGAAACAAGTTGAAGAGTTGTTC
  GAAAGAAAATATGCTCAAGCCATCAAAGCCAAAGGCCCGGTGACGATCCCGTACCCTCTTTTCCAGTCCC
  ATGTTGAAGATCTTTACGTAGAAGGGCTTCCTGAAGGGATTCCTTTTAGAAGGCCGTCGACGTACGGCAT
  TCCTCGCCTTGAGAGGATATTGCTGGCGAAGGAAAGGATCCGCTTCGTGATTAAGAAACATGAGCTTCTG
  AACTCAACACGAGAAGATTTACAGCTTGATAAACCAGCCTCAGGAGTGAAAGAAGAGTGGTACGCTCGCA
  TCACCAAACTGAGGAAGATGGTTGACCAGCTCTTCTGCAAGAAATTTGCCGAGGCCTTGGGAAGCACGGA
  AGCCAAGGCTGTACCGTACCAAAAATTTGAGGCCCATCCCAATGATCTCTATGTGGAAGGACTTCCAGAA
  AACATTCCTTTCCGAAGCCCCTCGTGGTATGGAATCCCAAGACTGGAAAAAATCATCCAAGTGGGCAATC
  GAATTAAATTTGTTATCAAGAGACCAGAGCTGCTCACTCACAGCACAACTGAAGTGACTCAGCCACGGAC
  AAACACACCAGTCAAAGAAGATTGGAATGTCAGAATCACCAAGCTCCGAAAGCAAGTGGAAGAGATCTTT
  AATTTGAAGTTTGCTCAGGCTCTGGGCCTCACAGAGGCTGTGAAGGTGCCATATCCTGTGTTCGAATCCA
  ACCCGGAGTTTCTGTATGTGGAAGGGCTCCCCGAAGGGATCCCCTTCCGAAGCCCTACCTGGTTTGGGAT
  TCCACGCCTCGAGAGGATTGTCCGTGGCAGCAATAAGATCAAGTTTGTTGTTAAAAAGCCTGAGCTAGTT
  GTGTCCTATTTGCCTCCTGGGATGGCTAGCAAAATCAACACTAAAGCATTGCAGTCCCCAAAACGACCAC
  GAAGCCCTGGGAGCAACTCCAAGGTTCCTGAAATTGAGGTCACTGTGGAAGGCCCCAACAACAGCAGTCC
  TCAGACCTCTGCGGTTCGAACTCCTACCCAGACCAATGGTTCAAATGTTCCCTTCAAGCCTCGAGGGAGA
  GAGTTTTCCTTTGAGGCCTGGAATGCTAAGATCACAGACCTGAAGCAGAAAGTGGAGAACCTCTTCAATG
  AAAAGTGCGGGGAAGCGCTTGGCCTTAAGCAGGCTGTGAAAGTCCCGTTTGCGTTGTTCGAGTCCTTCCC
  AGAAGACTTCTATGTGGAAGGCTTGCCCGAGGGGGTGCCCTTCCGAAGGCCGTCCACATTTGGCATTCCC
  AGGCTGGAGAAGATACTCCGGAACAAGGCCAAGATAAAGTTCATCATTAAAAAACCTGAGATGTTCGAGA
  CGGCCATCAAAGAGAGCACCTCTTCCAAAAGCCCTCCAAGAAAGATAAACTCATCACCCAACGTTAATAC
  TACTGCATCAGGTGTGGAAGACCTGAACATCATCCAGGTGACAATTCCAGATGACGATAATGAACGACTG
  TCCAAAGTTGAAAAGGCCAGGCAGCTGCGGGAGCAGGTCAACGACCTCTTCAGTCGGAAGTTTGGTGAAG
  CTATTGGGATGGGCTTCCCGGTGAAGGTCCCCTACAGGAAGATCACCATCAACCCTGGCTGCGTGGTGGT
  CGATGGCATGCCCCCCGGGGTGTCCTTCAAAGCCCCCAGTTACCTGGAGATCAGCTCCATGAGGAGGATC
  TTAGACTCTGCGGAGTTTATCAAGTTCACTGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGC
  TGGTTGATCAGAACGAGTCTGAAGGCCCTGTGATACAAGAATCAGCCGAGGCAAGCCAGTTGGAAGTCCC
  AGTAACAGAAGAAATTAAAGAGACAGATGGGAGTTCACAGATCAAGCAGGAGCCAGACCCCACGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226048 representing NM_010365
  Red=Cloning site Green=Tags(s)
  
  MAQVVMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK
  DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK
  MLRDQSAVVVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVLAAEAERSMLS
  PSGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGTEVEVPAEDSTQHVP
  SETSEDPEVEVTIEDDDYSPPTKRLKSTEPPPPPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELF
  ERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELL
  NSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPE
  NIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIF
  NLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELV
  VSYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNSSPQTSAVRTPTQTNGSNVPFKPRGR
  EFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIP
  RLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERL
  SKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRI
  LDSAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPVTEEIKETDGSSQIKQEPDPTW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010365
• ORF Size: 2937 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010365.3, NP_034495.2
• RefSeq Size: 4384
• RefSeq ORF: 2940
• Locus ID: 14886