Dnmt3a (NM_153743) Mouse Tagged ORF Clone

CAT#: MG226152

DNMT3A (GFP-tagged) - Mouse DNA methyltransferase 3A (DNMT3A) transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnmt3a
• Synonyms: MmuIIIA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226152 representing NM_153743
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTGTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTG
  TGCAGCAGCCCACGGACCCTGCTTCTCCGACTGTGGCCACCACCCCTGAGCCAGTAGGAGGGGATGCTGG
  GGACAAGAATGCTACCAAAGCAGCCGACGATGAGCCTGAGTATGAGGATGGCCGGGGCTTTGGCATTGGA
  GAGCTGGTGTGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAGGCCGAATTGTGTCTTGGTGGATGACAG
  GCCGGAGCCGAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTTCGGAGATGGCAAGTTCTCAGTGGTGTG
  TGTGGAGAAGCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTCCACCAGGCCACCTACAACAAGCAGCCC
  ATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCAGCCGTGCCGGGAAGCTGTTTCCAGCTT
  GCCATGACAGTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGTGCAGAACAAGCAGATGATTGAATGGGC
  CCTCGGTGGCTTCCAGCCCTCGGGTCCTAAGGGCCTGGAGCCACCAGAAGAAGAGAAGAATCCTTACAAG
  GAAGTTTACACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTTACGCCCCACCCCCACCAGCCAAGAAAC
  CCAGAAAGAGCACAACAGAGAAACCTAAGGTCAAGGAGATCATTGATGAGCGCACAAGGGAGCGGCTGGT
  GTATGAGGTGCGCCAGAAGTGCAGAAACATCGAGGACATTTGTATCTCATGTGGGAGCCTCAATGTCACC
  CTGGAGCACCCACTCTTCATTGGAGGCATGTGCCAGAACTGTAAGAACTGCTTCTTGGAGTGTGCTTACC
  AGTATGACGACGATGGGTACCAGTCCTATTGCACCATCTGCTGTGGGGGGCGTGAAGTGCTCATGTGTGG
  GAACAACAACTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGATCTCTTGGTGGGGCCAGGAGCTGCTCAG
  GCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCATAAGGGCACCTATGGGCTGCTGCGAA
  GACGGGAAGACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAATAACCATGACCAGGAATTTGACCCCCC
  AAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATCCGCGTGCTGTCTCTCTTTGATGGGATT
  GCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGGACCGCTACATTGCCTCCGAGGTGTGTG
  AGGACTCCATCACGGTGGGCATGGTGCGGCACCAGGGAAAGATCATGTACGTCGGGGACGTCCGCAGCGT
  CACACAGAAGCATATCCAGGAGTGGGGCCCATTCGACCTGGTGATTGGAGGCAGTCCCTGCAATGACCTC
  TCCATTGTCAACCCTGCCCGCAAGGGACTTTATGAGGGTACTGGCCGCCTCTTCTTTGAGTTCTACCGCC
  TCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTCTTTGAGAATGTGGTGGC
  CATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTCTTGAGTCTAACCCCGTGATGATTGACGCCAAA
  GAAGTGTCTGCTGCACACAGGGCCCGTTACTTCTGGGGTAACCTTCCTGGCATGAACAGGCCTTTGGCAT
  CCACTGTGAATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCACGGCAGAATAGCCAAGTTCAGCAAAGT
  GAGGACCATTACCACCAGGTCAAACTCTATAAAGCAGGGCAAAGACCAGCATTTCCCCGTCTTCATGAAC
  GAGAAGGAGGACATCCTGTGGTGCACTGAAATGGAAAGGGTGTTTGGCTTCCCCGTCCACTACACAGACG
  TCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGATCGTGGAGCGTGCCGGTCATCCGCCA
  CCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226152 representing NM_153743
  Red=Cloning site Green=Tags(s)
  
  MNAVEENQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGGDAGDKNATKAADDEPEYEDGRGFGIG
  ELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQP
  MYRKAIYEVLQVASSRAGKLFPACHDSDESDSGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYK
  EVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVT
  LEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQ
  AAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGI
  ATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDL
  SIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAK
  EVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMN
  EKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153743
• ORF Size: 2067 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153743.4, NP_714965.1
• RefSeq Size: 8913
• RefSeq ORF: 2070
• Locus ID: 13435
• Gene Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]