Cpe (NM_013494) Mouse Tagged ORF Clone

CAT#: MG226559

Cpe (GFP-tagged) - Mouse carboxypeptidase E (Cpe), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cpe
• Synonyms: CPH; Cph-1; Cph1; fat; NF-alpha1; R74677
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226559 representing NM_013494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCGCGGAGGACGGGTGCTGCTGGCGCTGTGTGCCGCGCTGGTGGCCGGCGGGTGGCTGCTGA
  CGGCTGAAGCCCAGGAGCCCGGGGCGCCAGCGGCTGGCATGAGGCGCCGCCGGCGGCTCCAGCAAGAGGA
  CGGCATCTCCTTCGAGTACCACCGCTATCCAGAGCTGCGCGAGGCGCTGGTGTCCGTATGGCTGCAGTGC
  ACCGCCATCAGCAGAATCTACACAGTGGGGCGCAGCTTCGAGGGCCGGGAGCTCCTGGTCATCGAGCTGT
  CTGACAACCCCGGGGTCCATGAGCCGGGTGAACCTGAATTTAAATACATTGGGAACATGCATGGTAATGA
  GGCGGTTGGACGGGAACTGCTTATTTTCTTGGCCCAGTACCTGTGTAACGAGTACCAGAAAGGCAATGAG
  ACAATTGTCAACCTGATCCACAGCACCCGAATTCATATCATGCCCTCCTTGAACCCCGACGGCTTTGAGA
  AAGCCGCATCGCAGCCCGGCGAGCTGAAGGACTGGTTTGTGGGCCGCAGCAACGCCCAGGGAATAGATCT
  GAACCGTAACTTCCCAGACCTGGACAGGATCGTGTATGTTAATGAGAAAGAAGGCGGTCCTAACAATCAC
  CTGCTGAAGAATCTGAAGAAAATTGTGGACCAAAATTCAAAGCTTGCCCCCGAGACCAAGGCTGTCATTC
  ACTGGATCATGGACATTCCATTTGTGCTTTCTGCCAATCTGCACGGAGGAGACCTTGTGGCTAATTACCC
  ATATGATGAGACACGGAGCGGTACTGCTCACGAATACAGTTCCTGCCCTGATGACGCAATTTTCCAAAGC
  TTGGCTCGCGCGTACTCTTCTTTCAACCCAGTCATGTCTGACCCCAATCGACCTCCCTGTCGCAAGAATG
  ACGATGACAGCAGCTTTGTAGATGGAACGACCAATGGTGGTGCATGGTACAGCGTCCCCGGTGGAATGCA
  AGACTTCAATTACCTGAGCAGCAACTGCTTCGAGATCACTGTGGAGCTTAGCTGTGAGAAGTTCCCACCG
  GAAGAGACTCTCAAAAGCTACTGGGAAGATAACAAAAACTCCCTCATCAGCTACCTGGAGCAGATACACC
  GAGGTGTTAAAGGGTTTGTCCGTGACCTTCAGGGTAACCCGATTGCCAACGCAACCATCTCTGTGGACGG
  GATAGACCATGATGTCACCTCGGCTAAGGATGGGGATTACTGGCGATTGCTTGCTCCTGGAAACTATAAA
  CTTACAGCCTCCGCTCCTGGCTACCTGGCAATCACAAAGAAAGTGGCAGTTCCTTTTAGCCCTGCTGTTG
  GGGTGGACTTTGAGCTTGAGTCTTTCTCTGAAAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTG
  GAAAATGATGTCAGAAACTTTGAATTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226559 representing NM_013494
  Red=Cloning site Green=Tags(s)
  
  MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQC
  TAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNE
  TIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNH
  LLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDAIFQS
  LARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPP
  EETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVDGIDHDVTSAKDGDYWRLLAPGNYK
  LTASAPGYLAITKKVAVPFSPAVGVDFELESFSERKEEEKEELMEWWKMMSETLNF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013494
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013494.3, NP_038522.2
• RefSeq Size: 2103
• RefSeq ORF: 1431
• Locus ID: 12876
• Gene Summary: This gene encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin. [provided by RefSeq, Jan 2016]