Chuk (NM_007700) Mouse Tagged ORF Clone

CAT#: MG226596

Chuk (GFP-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (Chuk) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chuk
• Synonyms: AI256658; Chuk1; Fbx24; Fbxo24; IKBKA; IKK1; Ikka; IKK alpha; NFKBIKA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226596 representing NM_007700
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGCCCCTGGGAGATGCGGGAACGGCTTGGCACCG
  GCGGTTTCGGGAACGTCAGTCTGTACCAGCACCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCG
  TTTAGAGCTAAGTTCCAAAAACAGAGAGCGATGGTGCCATGAAATCCAGATCATGAAAAAGTTGGACCAT
  GCGAATGTTGTAAAGGCCTGTGATGTCCCTGAGGAATTGAACTTTTTAATTAACGATGTGCCTCTTCTGG
  CAATGGAGTACTGTTCTGGAGGGGACCTCCGGAAGCTACTCAACAAACCAGAAAATTGTTGTGGACTTAA
  AGAAAGCCAGATACTTTCTTTACTGAGTGACATAGGATCTGGGATCCGATATCTGCATGAAAACAAAATT
  ATACATCGAGATCTAAAACCTGAAAATATAGTTCTTCAAGATGTTGGTGGGAAGACAATACATAAAATAA
  TTGATTTGGGTTATGCCAAAGATGTTGATCAAGGAAGTCTCTGTACATCTTTTGTGGGAACATTGCAGTA
  TTTGGCCCCAGAGCTCTTTGAAAATAAGCCGTACACAGCCACTGTGGATTATTGGAGCTTTGGGACCATG
  GTGTTTGAATGTATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGA
  TTAAGAAGAAAGATCCAAAGTGTATATTTGCATGTGAAGAGATGACTGGAGAAGTTCGGTTTAGTAGCCA
  TTTACCTCAGCCAAACAGCCTTTGTAGTTTAATAGTAGAGCCAATGGAAAGCTGGCTCCAATTGATGCTG
  AATTGGGACCCACAGCAGAGAGGGGGACCTATTGATCTTACTTTGAAGCAGCCAAGATGTTTTGCATTAA
  TGGATCACATTCTCAATTTAAAGATAGTGCACATCCTAAATATGACTTCTGCAAAAATCATTTCTTTTCT
  GTTACCATGTGATGAAAGTCTTCATTCACTACAGTCTCGAATTGAGCGTGAAACAGGAATAAATACAGGT
  TCTCAGGAGCTTCTGTCAGAGACAGGGATTTCTCTGGATCCTCGGAAACCAGCCTCTCAGTGTGTTCTAG
  ATGGAGTTAGAGGCTGTGATAGCTACATGGTTTATTTGTTTGATAAAAGTAAGACTGTATATGAAGGACC
  ATTTGCATCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAAGACAGCAAAATACAACTGCCAATT
  ATACAGCTGCGGAAAGTATGGGCTGAAGCAGTGCACTACGTATCTGGGCTAAAGGAAGACTACAGCAGGC
  TCTTCCAGGGACAAAGAGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTGACAAAAATGAAGAA
  TACTTTGATCTCAGCATCACAGCAACTCAAAGCTAAATTGGAGTTTTTTCGAAAAAGCATTCAGCTTGAC
  TTGGAGAGATATAGTGAGCAGATGACTTATGGGATATCTTCAGAAAAAATGTTAAAAGCATGGAAAGAAA
  TGGAAGAAAAGGCCATTCACTATTCTGAGGTTGGTGTCATTGGTTATCTTGAGGATCAAATTATGTCTTT
  GCACACTGAAATCATGGAGCTGCAGAAGAGCCCCTATGGACGACGCCAGGGAGACTTGATGGAGTCTCTG
  GAGCAGCGTGCCATTGATCTCTATAAGCAGCTAAAGCACAGACCTCCTGATCACTTGTACAGCGACAGCA
  CAGAGATGGTGAAGATCATCGTGCACACCGTGCAGAGTCAGGACCGTGTTCTCAAGGAGCTGTTTGGTCA
  CCTGAGCAAGTTGTTGGGCTGCAAGCAGAAGATTATTGATCTACTCCCCAAGGTGGAAGTGGCCCTCAGT
  AACATCAAAGAAGCTGACAATACTGTCATGTTTATGCAGGGAAAGAGGCAGAAAGAAATTTGGCACCTCC
  TTAAAATTGCCTGTACACAGAGTTCTGCCCGCTCTCTTGTAGGATACAGTCTAGAAGGCACAGTAACCCC
  TCCAGTATCAGCATGGCTGCCCCCTACATTAGCAGACCGTGAACATCCTCTGACATGTGTGGTAACTCCT
  CAAGATGGAGAGACGTTAGCACAAATGATAGAAGAAAATCTGAACTGTCTTGGCCATTTAAGTACTATTA
  TTCGTGAAGCAAATGAGGACCAGAGCAGTAGTTTGATGAGTCTTGATTGGAGTTGGTTAGCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226596 representing NM_007700
  Red=Cloning site Green=Tags(s)
  
  MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSSKNRERWCHEIQIMKKLDH
  ANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKI
  IHRDLKPENIVLQDVGGKTIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTM
  VFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMESWLQLML
  NWDPQQRGGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCDESLHSLQSRIERETGINTG
  SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPI
  IQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLD
  LERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESL
  EQRAIDLYKQLKHRPPDHLYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALS
  NIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGYSLEGTVTPPVSAWLPPTLADREHPLTCVVTP
  QDGETLAQMIEENLNCLGHLSTIIREANEDQSSSLMSLDWSWLAE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007700
• ORF Size: 2235 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007700.2, NP_031726.2
• RefSeq Size: 3500
• RefSeq ORF: 2238
• Locus ID: 12675