Comt (NM_001111062) Mouse Tagged ORF Clone

CAT#: MG226619

Comt (GFP-tagged) - Mouse catechol-O-methyltransferase 1 (Comt1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Comt
• Synonyms: Comt1; D16Wsu103e; D330014B15Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226619 representing NM_001111062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTGGCTGCTGTCTCATTGGGTCTCCTGTTGCTGGCCTTCCTCCTGCTCCTGCGACACCTAGGCT
  GGGGCTTGGTGGCTATTGGTTGGTTTGAGTTCGTGCAGCAGCCGGTCCACAACCTGCTCATGGGTGGCAC
  AAAGGAGCAGCGCATCCTGCGCCATGTGCAGCAACACGCAAAGCCTGGAGACCCCCAGAGCGTCCTGGAG
  GCCATTGATACCTACTGCTCAGAGAAGGAGTGGGCCATGAACGTGGGTGACGCAAAAGGCCAAATCATGG
  ATGCAGTGATTCGGGAGTACAGGCCCTCGCTGGTGCTGGAGCTAGGAGCTTATTGTGGCTACTCAGCCGT
  GCGAATGGCCCGCCTGCTGCCACCTGGAGCCAGGCTTCTCACCATGGAGATTAACCCTGACTACGCTGCC
  ATCACCCAGCAAATGCTGGACTTCGCAGGCCTACAGGACAAAGTTTCCATCCTCATCGGGGCATCCCAGG
  ACCTTATCCCCCAGCTGAAGAAGAAGTACGATGTGGACACATTAGACATGGTCTTTCTTGACCACTGGAA
  AGACCGCTACCTTCCAGACACACTTCTCCTGGAGGAATGTGGCCTGCTGCGCAAGGGGACGGTGCTCCTA
  GCTGACAATGTCATTGTCCCGGGAACCCCTGACTTCCTGGCGTATGTGAGGGGGAGCAGCAGCTTCGAGT
  GCACACACTACAGCTCATACCTGGAGTACATGAAAGTGGTGGACGGCTTGGAGAAGGCAGTCTACCAGGG
  TCCAGGCAGCAGCCCCGTGAAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226619 representing NM_001111062
  Red=Cloning site Green=Tags(s)
  
  MLLAAVSLGLLLLAFLLLLRHLGWGLVAIGWFEFVQQPVHNLLMGGTKEQRILRHVQQHAKPGDPQSVLE
  AIDTYCSEKEWAMNVGDAKGQIMDAVIREYRPSLVLELGAYCGYSAVRMARLLPPGARLLTMEINPDYAA
  ITQQMLDFAGLQDKVSILIGASQDLIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLL
  ADNVIVPGTPDFLAYVRGSSSFECTHYSSYLEYMKVVDGLEKAVYQGPGSSPVKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111062
• ORF Size: 795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001111062.1, NP_001104532.1
• RefSeq Size: 2185
• RefSeq ORF: 798
• Locus ID: 12846
• Gene Summary: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. [UniProtKB/Swiss-Prot Function]