Akt2 (NM_001110208) Mouse Tagged ORF Clone

CAT#: MG226761

Akt2 (GFP-tagged) - Mouse thymoma viral proto-oncogene 2 (Akt2) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Akt2
• Synonyms: 2410016A19Rik; AW554154; PKB; PKBbeta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226761 representing NM_001110208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAGGTATCTGTCATCAAAGAAGGCTGGCTCCACAAACGTGGTGAATACATCAAGACCTGGAGGC
  CACGGTACTTCCTTCTGAAGAGTGATGGATCTTTCATTGGGTATAAGGAGAGGCCCGAGGCCCCTGACCA
  GACCTTACCCCCCCTGAACAATTTCTCTGTAGCAGAATGCCAGCTGATGAAGACTGAGAGGCCACGACCC
  AACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCATGTAGACTCTCCAG
  ATGAGAGGGAAGAGTGGATGCGGGCTATCCAGATGGTCGCCAACAGTCTGAAGCAGCGGGGCCCAGGTGA
  GGACGCCATGGATTACAAGTGTGGCTCCCCCAGTGACTCTTCCACATCTGAGATGATGGAGGTAGCTGTC
  AACAAGGCACGGGCCAAAGTGACCATGAATGACTTCGATTATCTCAAACTCCTCGGCAAGGGCACCTTCG
  GCAAGGTCATTCTGGTTCGAGAGAAGGCCACTGGCCGCTATTATGCCATGAAGATCCTGCGCAAGGAGGT
  CATCATTGCAAAGGATGAAGTCGCCCACACAGTCACAGAGAGCCGGGTTCTGCAGAATACCAGGCACCCC
  TTCCTTACAGCCCTCAAGTATGCCTTCCAGACCCATGACCGCCTATGCTTTGTGATGGAGTATGCCAACG
  GGGGTGAGCTGTTTTTCCACCTCTCTCGGGAGCGAGTCTTCACGGAGGATCGGGCGCGCTTTTATGGAGC
  AGAGATTGTGTCAGCTCTGGAGTATTTGCACTCGAGAGATGTGGTGTACCGTGACATCAAGCTGGAAAAC
  CTTATGTTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCTTGTGCAAAGAGGGCATCAGTGATG
  GAGCCACCATGAAAACCTTCTGTGGTACCCCGGAGTACTTGGCGCCTGAGGTGCTAGAGGACAATGACTA
  TGGGCGAGCAGTGGACTGGTGGGGGCTGGGTGTGGTCATGTATGAGATGATGTGTGGCCGCCTGCCATTC
  TACAACCAGGACCACGAGCGCCTCTTTGAGCTCATTCTTATGGAGGAGATCCGCTTCCCGCGCACACTCG
  GGCCAGAGGCCAAGTCCCTGCTGGCTGGACTGCTGAAGAAGGACCCAAAGCAGAGGCTCGGCGGAGGTCC
  CAGTGATGCGAAGGAGGTCATGGAGCATAGATTCTTCCTCAGCATCAACTGGCAGGACGTGGTACAGAAA
  AAGCTCCTGCCACCCTTCAAACCTCAGGTCACTTCAGAAGTGGACACAAGGTACTTTGATGACGAGTTCA
  CCGCCCAGTCCATCACAATCACACCCCCAGACCGATATGACAGCCTGGACCCGCTGGAACTGGACCAGCG
  GACGCACTTCCCCCAGTTCTCCTACTCAGCCAGCATCCGAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226761 representing NM_001110208
  Red=Cloning site Green=Tags(s)
  
  MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRP
  NTFVIRCLQWTTVIERTFHVDSPDEREEWMRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAV
  NKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHP
  FLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLEN
  LMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPF
  YNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK
  KLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLDPLELDQRTHFPQFSYSASIRE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110208
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001110208.1, NP_001103678.1
• RefSeq Size: 3199
• RefSeq ORF: 1446
• Locus ID: 11652
• Gene Summary: One of the few specific substrates of AKT2 identified so far is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Phosphorylates CLK2 on 'Thr-343'. [UniProtKB/Swiss-Prot Function]