Akt2 (NM_001110208) Mouse Tagged ORF Clone

CAT#: MR226761L4

Lenti ORF clone of Akt2 (mGFP-tagged) - Mouse thymoma viral proto-oncogene 2 (Akt2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: mGFP
• Symbol: Akt2
• Synonyms: 2410016A19Rik; AW554154; PKB; PKBbeta
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR226761).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_001110208
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001110208.1, NP_001103678.1
• RefSeq Size: 3199
• RefSeq ORF: 1446
• Locus ID: 11652
• Gene Summary: One of the few specific substrates of AKT2 identified so far is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Phosphorylates CLK2 on 'Thr-343'. [UniProtKB/Swiss-Prot Function]