Myh6 (NM_001164171) Mouse Tagged ORF Clone

CAT#: MG226784

Myh6 (GFP-tagged) - Mouse myosin heavy polypeptide 6 cardiac muscle alpha (Myh6) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Myh6
• Synonyms: A830009F23Rik; AA517445; alpha-MHC; alphaMHC; Myhc-a; Myhca
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226784 representing NM_001164171
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGACGCCCAGATGGCTGACTTCGGGGCAGCAGCCCAGTACCTCCGAAAGTCAGAGAAGGAACGCC
  TAGAGGCCCAGACCCGGCCCTTTGACATCCGCACGGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGT
  TAAGGCCAAGGTCGTGTCCCGGGAAGGGGGCAAAGTCACTGCGGAAACTGAAAACGGAAAGACGGTGACC
  ATAAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA
  CCTTCCTGCACGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCGGCCTGGATGATCTATACCTA
  CTCAGGCCTCTTCTGCGTCACCGTCAACCCCTATAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCC
  GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT
  ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCGGGGAAGACTGTGAA
  CACAAAACGTGTCATCCAGTACTTTGCCAGCATTGCAGCCATAGGGGACCGTAGCAAGAAGGAAAATCCT
  AATGCAAACAAGGGCACCCTGGAGGACCAGATTATCCAGGCTAACCCCGCTCTGGAGGCCTTCGGCAACG
  CCAAGACTGTCCGGAATGACAACTCCTCCCGCTTTGGGAAATTCATCAGGATCCACTTTGGAGCTACTGG
  AAAGCTGGCTTCTGCAGACATAGAGACCTACCTTCCGGAGAAGTCCCGGGTGATCTTCCAGCTAAAGGCT
  GAGAGGAACTACCACATCTTCTACCAGATCCTGTCCAACAAGAAGCCGGAGCTGCTGGACATGCTGCTGG
  TCACCAACAACCCATACGACTACGCCTTCGTCTCTCAGGGAGAGGTGTCCGTGGCCTCCATTGATGACTC
  TGAGGAGCTCTTGGCCACTGATAGTGCCTTTGATGTGCTGAGCTTCACGGCAGAGGAGAAGGCTGGTGTC
  TACAAGCTGACAGGGGCCATCATGCACTACGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG
  CGGAGCCTGATGGCACAGAAGATGCTGACAAATCTGCCTACCTTATGGGGCTGAACTCAGCTGACCTGCT
  CAAGGGCCTGTGTCACCCTCGGGTGAAGGTGGGGAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAA
  GTGTACTATTCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCA
  TCAACGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGA
  GATCTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTC
  AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGGAGTTTATCGACC
  TCGGCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTCGAGGA
  GGAGTGCATGTTCCCCAAGGCCTCAGACATGACCTTCAAGGCCAAGCTGTATGACAACCACCTGGGCAAA
  TCCAACAACTTCCAGAAGCCTCGCAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCCTTGGTCCACTATG
  CTGGCACCGTGGACTACAACATTATGGGCTGGCTGGAAAAGAACAAGGACCCACTCAATGAGACGGTGGT
  GGGTTTGTACCAGAAGTCCTCCCTCAAGCTCATGGCTACACTCTTCTCTACCTATGCTTCTGCTGATACC
  GGTGACAGTGGTAAAGGCAAAGGAGGCAAGAAGAAAGGCTCATCCTTCCAAACAGTGTCTGCTCTCCACC
  GGGAAAATCTGAACAAGCTGATGACAAACCTGAAGACCACCCACCCTCACTTTGTGCGCTGCATCATTCC
  CAACGAGCGAAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTG
  CTGGAGGGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGT
  ATCGCATCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACT
  GCTGGGCTCCCTGGACATTGACCACAACCAATACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCGGGC
  CTGCTGGGGCTGCTCGAGGAGATGCGAGATGAGAGGCTGAGCCGTATCATCACCAGAATCCAGGCCCAGG
  CCCGAGGGCAGCTCATGCGCATTGAGTTCAAGAAGATAGTGGAACGCAGGGATGCCCTGCTGGTTATCCA
  GTGGAACATTCGGGCCTTCATGGGGGTCAAGAATTGGCCATGGATGAAGCTCTACTTCAAGATCAAACCG
  CTGCTGAAGAGCGCAGAGACGGAGAAGGAGATGGCCAACATGAAGGAGGAGTTTGGGCGAGTCAAAGATG
  CACTGGAGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA
  TGACCTACAGCTCCAAGTGCAGGCGGAACAAGACAACCTCAATGATGCAGAGGAGCGCTGTGACCAGCTG
  ATCAAGAACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGA
  TGAACGCCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGAAGGATATTGA
  TGACCTGGAGCTGACGCTGGCCAAGGTGGAAAAGGAAAAGCATGCAACAGAGAACAAGGTTAAAAACCTA
  ACAGAGGAGATGGCTGGGCTGGATGAAATCATTGCCAAGCTGACCAAAGAGAAGAAAGCTCTGCAAGAAG
  CCCACCAGCAAGCCCTCGATGACCTGCAGGCTGAAGAAGACAAGGTCAACACGCTGACCAAGTCCAAAGT
  CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAAGTGCGCATGGACCTA
  GAGCGAGCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAATG
  ACAAGCTTCAGCTGGAAGAAAAGCTCAAGAAGAAAGAGTTCGACATCAGTCAGCAGAACAGTAAAATTGA
  GGACGAGCAGGCCCTGGCTCTTCAGCTGCAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG
  GAGGAGGAGCTGGAGGCAGAGCGCACAGCCCGGGCTAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGG
  AGCTGGAGGAGATCAGTGAGAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAATAA
  GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACG
  GCGGCGGCCCTGCGCAAGAAGCATGCTGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGC
  GGGTGAAGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACAT
  GGAGCAGATCATCAAGGCCAAGGCCAACCTGGAGAAGGTGTCCCGGACACTGGAGGACCAGGCCAATGAG
  TACCGCGTGAAGCTGGAAGAAGCCCAGCGCTCCCTCAATGACTTCACCACACAGCGAGCCAAGCTGCAGA
  CAGAGAACGGGGAGTTGGCTAGGCAACTGGAAGAAAAGGAGGCATTGATTTCCCAGCTGACCCGAGGCAA
  GCTCTCCTACACCCAGCAGATGGAGGACCTCAAGAGGCAACTGGAGGAGGAAGGCAAGGCCAAGAACGCC
  CTGGCCCACGCACTGCAATCATCCCGGCATGACTGTGACCTGCTGAGGGAACAGTATGAAGAAGAAATGG
  AGGCCAAGGCTGAGCTACAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTA
  TGAGACGGATGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAG
  GATGCAGAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGTTCCTCCCTGGAGAAGACCAAGCACAGGCTGC
  AGAATGAGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAA
  GCAGAGGAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAG
  TCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTC
  TGGAGCACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACA
  GCTGGGAGAAGGGGGGAAAAACGTGCACGAGCTGGAGAAGATCCGCAAACAGCTGGAGGTGGAGAAGCTG
  GAGCTGCAGTCAGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGTGCCC
  AGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCA
  GGCCAAGCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGC
  AATGAGGCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCAGG
  CCAATAGAATAGCCTCAGAGGCACAGAAACACCTGAAGAATTCTCAAGCTCACTTGAAGGACACCCAGCT
  CCAGCTGGATGATGCTGTCCATGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAACGGCGCAAC
  AACCTGCTGCAGGCGGAGCTGGAGGAGCTGCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGG
  CAGAGCAGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAA
  CCAGAAGAAGAAGATGGAGTCAGACCTGACCCAACTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGT
  AGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCAATGATGGCTGAGGAGCTGAAGAAGG
  AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCA
  CCGTCTGGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGG
  GTCCGGGAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCAGAGTCGGTGAAGGGCATGAGGA
  AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAGAAGAACTTAATGCGGCTGCA
  GGACCTGGTGGACAAGCTACAGTTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAG
  GCCAACACCTACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACA
  TCGCCGAGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGAAGATGCACGACGA
  GGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226784 representing NM_001164171
  Red=Cloning site Green=Tags(s)
  
  MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKVVSREGGKVTAETENGKTVT
  IKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVA
  AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENP
  NANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLPEKSRVIFQLKA
  ERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGV
  YKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQ
  VYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFF
  NHHMFVLEQEEYKKEGIEWEFIDLGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGK
  SNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADT
  GDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGV
  LEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAG
  LLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKP
  LLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQL
  IKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNL
  TEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDL
  ERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEEL
  EEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEAT
  AAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANE
  YRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNA
  LAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQ
  DAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELE
  SSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKL
  ELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSR
  NEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRN
  NLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQEC
  RNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEAR
  VRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQ
  ANTYLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKKMHDEE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164171
• ORF Size: 5814 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164171.1, NP_001157643.1
• RefSeq Size: 6079
• RefSeq ORF: 5817
• Locus ID: 17888
• Gene Summary: Muscle contraction. [UniProtKB/Swiss-Prot Function]