Pola1 (NM_008892) Mouse Tagged ORF Clone

CAT#: MG226939

Pola1 (GFP-tagged) - Mouse polymerase (DNA directed) alpha 1 (Pola1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pola1
• Synonyms: AW321876; Pola
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226939 representing NM_008892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCATGCACGAAGAGGACTGTAAACTGGAGGCAAGCGCTGTGTCAGATTCAGGGAGTTTTGCAG
  CTTCCCGAGCTCGGCGAGAAAAGAAATCAAAGAAAGGACGTCAAGAAGCTTTAGAGAGACTGAAAAAGGC
  TAAAGCTGGGGAGAAGTATAAATACGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG
  CAGTATTCGAAACTGGTTCAGGCCCGTCAGGATGATGACTGGATTGTGGATGACGATGGGATAGGCTATG
  TGGAAGATGGCAGAGAGATTTTTGATGATGACCTTGAGGATGATGCACTTGACACCTGTGGAAAAGGAAG
  TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC
  AAGGCAATGTTCATTGCTAGTGCCGGGAAGAAAACTACAGATAAAGCAGTAGACTTGTCCAAGGATGATC
  TGCTAGGTGACATTCTACAGGATCTAAACACTGAGACAGCTCAAATAACTCCACCACCTGTATTGATACC
  AAAGAAAAAAAGATCTACTGGGGCTTTGCTCAACCCTTTTTCAGTGCACACCCCTAAGGCAATTCCTTCA
  GGAAAACCTGCTTCCCCTGTCCTCAGGAATGAGCCTCTGTTAACTCCCATTCCTCTTAAACGTGCTGAAC
  TTGCTGGAGAGCTGGCTCAGCCAGAATGTCCAGAAGATGAGCAGGAGCTAGGGGTAATGGAGTTTGAAGA
  CGGTGACTTTGATGAGTCCATGGACACAGAGAAGGTAGATGAGAAGCCTGTGACTGCTAAGACTTGGGAC
  CAAGAGACTGAGCCAGTAGAAAGGGTGGAACATGAGGCAGATCCTGAGAGGGGAACCACATCTTACTTAG
  AAAATTTTCTGCCAGATGTCTCTTGTTGGGACATTGATCAGGATGATGAAAGCATACCTCAGGAAGTTCA
  AGTGGATTCCAGTAATCTCCCATTGGTAAAAGGGGCAGATGATGAACAAGTGTTTCAGTTTTATTGGCTG
  GATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTGTGGATTGAATCAG
  TCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCTTCCCCGTGA
  AATGAAATTTGACCTAAACACAGGGAAAGAAACAGCAATTCCAGTTACAATGAAGGATGTTTATGAAGAA
  TTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAAAGAACTATG
  CTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACCTGGAAGTTAGATACTCGGCAGAAGTGCCACA
  GCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTGTTTGGAACTAACACGTCTAGCTTGGAACTG
  TTCTTGATGAATAGGAAGATCAAAGGACCTTGTTGGCTTGAAGTAAAAAATCCACAACTCTTGAATCAGC
  CAATCAGTTGGTGTAAATTTGAGGTAATGGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAG
  TCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAGAATGTACAGAACCATCAACATGAG
  ATTATTGCTATGGCGGCTTTGGTTCATCACAGTTTTGCATTAGATAAAGCACCTCCAGAGCCTCCCTTTC
  AGACACATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGTGATTTCAAAGAAGTGATTTC
  GAAAAAGAATATGAAGGTTGAGATCGCTGCAACAGAAAGAACCTTGATAGGATTTTTCCTTGCAAAAGTT
  CACAAAATTGACCCTGACATTCTTGTGGGTCATAATATTTGTAGTTTTGAACTGGAAGTGCTGCTGCAAA
  GAATTAATGAGTGCAAGGTTCCTTACTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACATGCCAAAACT
  TGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGCCGAATGATATGTGATGTGGAAATTTCA
  GCAAAAGAATTGATTCATTGTAAAAGCTACCATTTGTCTGAACTTGTTCAGCAGATTCTGAAAACTGAGA
  GGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAATCTTCTTATCTGTTGTACCTGTTGGA
  ACATATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCATTAGCATTG
  CAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGCGAAATGAGT
  TTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAACCTCA
  GCAAAAACTGGGAGATGAAGATGAGGAAATTGATGGGGACACCAATAAATACAAGAAAGGGCGTAAGAAA
  GCAACTTACGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTTTGCTCCTGG
  ATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCAAAGAGTTAC
  TTCAGAGGTGCAGAAAGCTACAGAGGATGAGGAACAAGAACAGATCCCTGAGTTGCCAGATCCAAACTTA
  GAGATGGGCATTTTGCCCAGAGAGATTCGGAAACTGGTAGAGCGGAGAAAACAAGTCAAACAGCTAATGA
  AACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGACATCCGACAGAAGGCTTTGAAGCTCACAGC
  AAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCACTGGCTGCCTTGGTG
  ACATACAAAGGAAGGGAGATTTTGATGCATACGAAAGACATGGTACAGAAGATGAATCTTGAAGTTATTT
  ATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTATTTAAGTTGGGAAA
  CAAGGTAAAAAGTGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGCTGTTTTCAAGTCT
  CTGCTGTTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTTGAACCAACATCAGATGGGAATTACATTA
  CCAAGCAGGAGCTGAAAGGATTAGATATAGTTAGAAGAGACTGGTGTGATCTTGCCAAGGACACTGGAAA
  CTTTGTCATTGGCCAGATTCTTTCTGATCAAAGCCGGGACACCATTGTGGAAAACATTCAGAAGAGGTTA
  ATAGAAATTGGAGAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATTGA
  CAAAGGATCCCCAGGATTACCCTGATAGAAAAAGCTTACCTCATGTACATGTTGCACTGTGGATAAATTC
  TCAAGGAGGCAGGAAGGTGAAAGCTGGTGATACGGTGTCCTATGTTATTTGTCAGGATGGGTCAAACCTC
  ACTGCAACTCAGAGGGCCTATGCCCCAGAACAATTGCAGAAACTTGACAATTTAGCCATCGACACCCAAT
  ACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGGATCTGTGAGCCAATAGATGGAATTGATGCTGT
  ACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAAGATGAAGAG
  AATGATGCCCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTGAAAAATTCA
  AATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGGACTGGATAT
  GGAACCCAGTTTGTATCGTTGCAGTAATGTTGATTGTAAGGTTTCACCGTTGACCTTCATGGTACAGCTG
  AGCAACAAATTGATCATGGACATTAGACGGTGCATTAAAAAGTACTATGATGGCTGGTTGATATGTGAAG
  AGCCAACTTGTTGCAGTCGCCTTCGACGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCTGTGTCCAGT
  CTGCATGAAAGCTGTGCTTAGACCAGAGTATTCTGACAAGTCCTTGTACACCCAGCTGTGCTTTTACCGG
  TACATTTTTGATGCGGACTGTGCACTGGAAAAACTTACTGAACATGAGAAAGATAAATTGAAGAAGCAGT
  TTTTCCCTCTCCGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAGCAGTTCTTGTCCTGGAG
  TGGCTACTCTGAAGTGAACCTCAGCAAGCTCTTCGCCAATTACGCTGGGAAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226939 representing NM_008892
  Red=Cloning site Green=Tags(s)
  
  MAPMHEEDCKLEASAVSDSGSFAASRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVDEE
  QYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGKAHRKDRKDVKKPSVTKPNNI
  KAMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTETAQITPPPVLIPKKKRSTGALLNPFSVHTPKAIPS
  GKPASPVLRNEPLLTPIPLKRAELAGELAQPECPEDEQELGVMEFEDGDFDESMDTEKVDEKPVTAKTWD
  QETEPVERVEHEADPERGTTSYLENFLPDVSCWDIDQDDESIPQEVQVDSSNLPLVKGADDEQVFQFYWL
  DAYEDPYNQPGVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKFDLNTGKETAIPVTMKDVYEE
  FDSKISAKYKIMKFKSKIVEKNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLEL
  FLMNRKIKGPCWLEVKNPQLLNQPISWCKFEVMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNHQHE
  IIAMAALVHHSFALDKAPPEPPFQTHFCVVSKPKDCIFPCDFKEVISKKNMKVEIAATERTLIGFFLAKV
  HKIDPDILVGHNICSFELEVLLQRINECKVPYWSKIGRLRRSNMPKLGSRSGFGERNATCGRMICDVEIS
  AKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLAL
  QITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKK
  ATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEVQKATEDEEQEQIPELPDPNL
  EMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALV
  TYKGREILMHTKDMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDAVFKS
  LLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRL
  IEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNL
  TATQRAYAPEQLQKLDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQYHKDEE
  NDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGLDMEPSLYRCSNVDCKVSPLTFMVQL
  SNKLIMDIRRCIKKYYDGWLICEEPTCCSRLRRLPLHFSRNGPLCPVCMKAVLRPEYSDKSLYTQLCFYR
  YIFDADCALEKLTEHEKDKLKKQFFPLRVLQDYRKVKNIAEQFLSWSGYSEVNLSKLFANYAGKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008892
• ORF Size: 4395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008892.2, NP_032918.1
• RefSeq Size: 5350
• RefSeq ORF: 4398
• Locus ID: 18968