Dhh (NM_007857) Mouse Tagged ORF Clone

CAT#: MG227142

Dhh (GFP-tagged) - Mouse desert hedgehog (Dhh), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dhh
• Synonyms: C78960
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227142 representing NM_007857
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCCGGCCAGTCTGTTGCCCCTGTGCTGCTTGGCACTCTTGGCACTATCTGCCCAGAGCTGCG
  GGCCGGGCCGAGGACCGGTTGGCCGGCGGCGTTATGTGCGCAAGCAACTTGTGCCTCTGCTATACAAGCA
  GTTTGTGCCCAGTATGCCCGAGCGGACCCTGGGCGCGAGTGGGCCAGCGGAGGGGAGGGTAACAAGGGGG
  TCGGAGCGCTTCCGGGACCTCGTACCCAACTACAACCCCGACATAATCTTCAAGGATGAGGAGAACAGCG
  GCGCAGACCGCCTGATGACAGAGCGTTGCAAAGAGCGGGTGAACGCTCTAGCCATCGCGGTGATGAACAT
  GTGGCCCGGAGTACGCCTACGTGTGACTGAAGGCTGGGACGAGGACGGCCACCACGCACAGGATTCACTC
  CACTACGAAGGCCGTGCCTTGGACATCACCACGTCTGACCGTGACCGTAATAAGTATGGTTTGTTGGCGC
  GCCTAGCTGTGGAAGCCGGATTCGACTGGGTCTACTACGAGTCCCGCAACCACATCCACGTATCGGTCAA
  AGCTGATAACTCACTGGCGGTCCGAGCCGGAGGCTGCTTTCCGGGAAATGCCACGGTGCGCTTGCGGAGC
  GGCGAACGGAAGGGGCTGAGGGAACTACATCGTGGTGACTGGGTACTGGCCGCTGATGCAGCGGGCCGAG
  TGGTACCCACGCCAGTGCTGCTCTTCCTGGACCGGGATCTGCAGCGCCGCGCCTCGTTCGTGGCTGTGGA
  GACCGAGCGGCCTCCGCGCAAACTGTTGCTCACACCCTGGCATCTGGTGTTCGCTGCTCGCGGGCCAGCG
  CCTGCTCCAGGTGACTTTGCACCGGTGTTCGCGCGCCGCTTACGTGCTGGCGACTCGGTGCTGGCTCCCG
  GCGGGGACGCGCTCCAGCCGGCGCGCGTAGCCCGCGTGGCGCGCGAGGAAGCCGTGGGCGTGTTCGCACC
  GCTCACTGCGCACGGGACGCTGCTGGTCAACGACGTCCTCGCCTCCTGCTACGCGGTTCTAGAGAGTCAC
  CAGTGGGCCCACCGCGCCTTCGCCCCTTTGCGGCTGCTGCACGCGCTCGGGGCTCTGCTCCCTGGGGGTG
  CAGTCCAGCCGACTGGCATGCATTGGTACTCTCGCCTCCTTTACCGCTTGGCCGAGGAGTTAATGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227142 representing NM_007857
  Red=Cloning site Green=Tags(s)
  
  MALPASLLPLCCLALLALSAQSCGPGRGPVGRRRYVRKQLVPLLYKQFVPSMPERTLGASGPAEGRVTRG
  SERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSL
  HYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHIHVSVKADNSLAVRAGGCFPGNATVRLRS
  GERKGLRELHRGDWVLAADAAGRVVPTPVLLFLDRDLQRRASFVAVETERPPRKLLLTPWHLVFAARGPA
  PAPGDFAPVFARRLRAGDSVLAPGGDALQPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESH
  QWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELMG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007857
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007857.4, NP_031883.1
• RefSeq Size: 2381
• RefSeq ORF: 1191
• Locus ID: 13363
• Gene Summary: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. [UniProtKB/Swiss-Prot Function]