Shh (NM_009170) Mouse Tagged ORF Clone

CAT#: MG227201

Shh (GFP-tagged) - Mouse sonic hedgehog (Shh), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Shh
• Synonyms: 9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081; ShhNC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227201 representing NM_009170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCTGCTGGCCAGATGTTTTCTGGTGATCCTTGCTTCCTCGCTGCTGGTGTGCCCCGGGCTGG
  CCTGTGGGCCCGGCAGGGGGTTTGGAAAGAGGCGGCACCCCAAAAAGCTGACCCCTTTAGCCTACAAGCA
  GTTTATTCCCAACGTAGCCGAGAAGACCCTAGGGGCCAGCGGCAGATATGAAGGGAAGATCACAAGAAAC
  TCCGAACGATTTAAGGAACTCACCCCCAATTACAACCCCGACATCATATTTAAGGATGAGGAAAACACGG
  GAGCAGACCGGCTGATGACTCAGAGGTGCAAAGACAAGTTAAATGCCTTGGCCATCTCTGTGATGAACCA
  GTGGCCTGGAGTGAAGCTGCGAGTGACCGAGGGCTGGGATGAGGACGGCCATCATTCAGAGGAGTCTCTA
  CACTATGAGGGTCGAGCAGTGGACATCACCACGTCCGACCGGGACCGCAGCAAGTACGGCATGCTGGCTC
  GCCTGGCTGTGGAAGCAGGTTTCGACTGGGTCTACTATGAATCCAAAGCTCACATCCACTGTTCTGTGAA
  AGCAGAGAACTCCGTGGCGGCCAAATCCGGCGGCTGTTTCCCGGGATCCGCCACCGTGCACCTGGAGCAG
  GGCGGCACCAAGCTGGTGAAGGACTTACGTCCCGGAGACCGCGTGCTGGCGGCTGACGACCAGGGCCGGC
  TGCTGTACAGCGACTTCCTCACCTTCCTGGACCGCGACGAAGGCGCCAAGAAGGTCTTCTACGTGATCGA
  GACGCTGGAGCCGCGCGAGCGCCTGCTGCTCACCGCCGCGCACCTGCTCTTCGTGGCGCCGCACAACGAC
  TCGGGGCCCACGCCCGGGCCAAGCGCGCTCTTTGCCAGCCGCGTGCGCCCCGGGCAGCGCGTGTACGTGG
  TGGCTGAACGCGGCGGGGACCGCCGGCTGCTGCCCGCCGCGGTGCACAGCGTGACGCTGCGAGAGGAGGA
  GGCGGGCGCGTACGCGCCGCTCACGGCGCACGGCACCATTCTCATCAACCGGGTGCTCGCCTCGTGCTAC
  GCTGTCATCGAGGAGCACAGCTGGGCACACCGGGCCTTCGCGCCTTTCCGCCTGGCGCACGCGCTGCTGG
  CCGCGCTGGCACCCGCCCGCACGGACGGCGGGGGCGGGGGCAGCATCCCTGCAGCGCAATCTGCAACGGA
  AGCGAGGGGCGCGGAGCCGACTGCGGGCATCCACTGGTACTCGCAGCTGCTCTACCACATTGGCACCTGG
  CTGTTGGACAGCGAGACCATGCATCCCTTGGGAATGGCGGTCAAGTCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227201 representing NM_009170
  Red=Cloning site Green=Tags(s)
  
  MLLLLARCFLVILASSLLVCPGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRN
  SERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESL
  HYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQ
  GGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHND
  SGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCY
  AVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTW
  LLDSETMHPLGMAVKSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009170
• ORF Size: 1311 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009170.3, NP_033196.1
• RefSeq Size: 2727
• RefSeq ORF: 1314
• Locus ID: 20423