Src (NM_001025395) Mouse Tagged ORF Clone

CAT#: MG227252

Src (GFP-tagged) - Mouse Rous sarcoma oncogene (Src) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Src
• Synonyms: AW259666; pp60c-src
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227252 representing NM_001025395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCAACAAGAGCAAGCCCAAGGACGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCTCGGAAAACG
  TGCACGGGGCAGGGGGCGCCTTCCCGGCCTCACAGACACCGAGCAAGCCCGCCTCCGCCGACGGCCACCG
  CGGGCCCAGCGCCGCCTTCGTGCCGCCCGCGGCCGAGCCCAAGCTCTTCGGAGGCTTCAACTCCTCGGAC
  ACCGTCACCTCCCCGCAGAGGGCGGGGCCTCTGGCAGGTGGGGTGACCACCTTTGTGGCCCTCTATGACT
  ATGAGTCACGGACAGAGACTGACCTGTCCTTCAAGAAAGGGGAGCGGCTGCAGATTGTCAATAACACAGA
  GGGAGACTGGTGGCTGGCACACTCGCTGAGCACGGGACAGACCGGTTACATCCCCAGCAACTATGTGGCG
  CCCTCCGACTCCATCCAGGCTGAGGAGTGGTACTTTGGCAAGATCACTAGACGGGAATCAGAGCGGCTGC
  TGCTCAACGCCGAGAACCCGAGAGGGACCTTCCTCGTGAGGGAGAGTGAGACCACAAAAGGTGCCTACTG
  CCTCTCTGTATCCGACTTCGACAATGCCAAGGGCCTAAATGTGAAACACTACAAGATCCGCAAGCTGGAC
  AGCGGCGGTTTCTACATCACCTCCCGCACCCAGTTCAACAGCCTGCAGCAGCTCGTGGCTTACTACTCCA
  AACATGCTGATGGCCTGTGTCACCGCCTCACTACCGTATGTCCCACATCCAAGCCTCAGACCCAGGGATT
  GGCCAAGGATGCGTGGGAGATCCCCCGGGAGTCCCTGCGGCTGGAGGTCAAGCTGGGCCAGGGTTGCTTC
  GGAGAGGTGTGGATGGGGACCTGGAACGGCACCACGAGGGTTGCCATCAAAACTCTGAAGCCAGGCACCA
  TGTCCCCAGAGGCCTTCCTGCAGGAGGCCCAAGTCATGAAGAAACTGAGGCACGAGAAACTGGTGCAGCT
  GTATGCTGTGGTGTCGGAAGAACCCATTTACATTGTGACAGAGTACATGAACAAGGGGAGTCTGCTGGAC
  TTTCTCAAGGGGGAAACGGGCAAATATTTGCGGCTACCCCAGCTGGTGGACATGTCTGCTCAGATCGCTT
  CAGGCATGGCCTATGTGGAGCGGATGAACTATGTGCACCGGGACCTTCGAGCCGCCAATATCCTAGTAGG
  GGAGAACCTGGTGTGCAAAGTGGCCGACTTTGGGTTGGCCCGGCTCATAGAAGACAACGAATACACAGCC
  CGGCAAGGTGCCAAATTCCCCATCAAGTGGACCGCCCCTGAAGCTGCTCTGTACGGCAGGTTCACCATCA
  AGTCGGATGTGTGGTCCTTTGGGATTCTGCTGACCGAGCTCACCACTAAGGGAAGAGTGCCCTATCCTGG
  GATGGTGAACCGTGAGGTTCTGGACCAGGTGGAGCGGGGCTACCGGATGCCTTGTCCCCCCGAGTGCCCC
  GAGTCCCTGCATGACCTTATGTGCCAGTGCTGGCGGAAGGAGCCCGAGGAGCGGCCCACCTTCGAGTACC
  TGCAGGCCTTCCTGGAAGACTACTTTACGTCCACTGAGCCACAGTACCAGCCCGGGGAGAACCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227252 representing NM_001025395
  Red=Cloning site Green=Tags(s)
  
  MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSD
  TVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVA
  PSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLD
  SGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCF
  GEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLD
  FLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTA
  RQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECP
  ESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025395
• ORF Size: 1605 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025395.2, NP_001020566.1
• RefSeq Size: 3869 bp
• RefSeq ORF: 1608 bp
• Locus ID: 20779
• Cytogenetics: 2 78.35 cM