Lck (NM_001162432) Mouse Tagged ORF Clone

CAT#: MG227319

Lck (GFP-tagged) - Mouse lymphocyte protein tyrosine kinase (Lck) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lck
• Synonyms: Hck-3; Lsk; Lskt; p56; p56Lck
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227319 representing NM_001162432
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCTCTGAGCTGACGATCTCGGGGATCATGGGCTGTGTCTGCAGCTCAAACCCTGAAGATGACT
  GGATGGAGAACATTGACGTGTGTGAAAACTGCCACTATCCCATAGTCCCACTGGACAGCAAGATCTCGCT
  GCCCATCCGGAATGGCTCTGAAGTGCGGGACCCACTGGTCACCTATGAGGGATCTCTCCCACCAGCATCC
  CCGCTGCAAGACAACCTGGTTATCGCCCTGCACAGTTATGAGCCCTCCCATGATGGAGACTTGGGCTTTG
  AGAAGGGTGAACAGCTCCGAATCCTGGAGCAGAGCGGTGAGTGGTGGAAGGCTCAGTCCCTGACGACTGG
  CCAAGAAGGCTTCATTCCCTTCAACTTCGTGGCGAAAGCAAACAGCCTGGAGCCTGAACCTTGGTTCTTC
  AAGAATCTGAGCCGTAAGGACGCCGAGCGGCAGCTTTTGGCGCCCGGGAACACGCATGGATCCTTCCTGA
  TCCGGGAAAGCGAAAGCACTGCGGGGTCCTTTTCCCTGTCGGTCAGAGACTTCGACCAGAACCAGGGAGA
  AGTGGTGAAACATTACAAGATCCGTAACCTAGACAACGGTGGCTTCTACATCTCCCCTCGTATCACTTTT
  CCCGGATTGCACGATCTAGTCCGCCATTACACCAACGCCTCTGATGGGCTGTGCACAAAGTTGAGCCGTC
  CTTGCCAGACCCAGAAGCCCCAGAAACCATGGTGGGAGGACGAATGGGAAGTTCCCAGGGAAACACTGAA
  GTTGGTGGAGCGGCTGGGAGCTGGCCAGTTCGGGGAAGTGTGGATGGGGTACTACAACGGACACACGAAG
  GTGGCGGTGAAGAGTCTGAAACAAGGGAGCATGTCCCCCGACGCCTTCCTGGCTGAGGCTAACCTCATGA
  AGCAGCTGCAGCACCCGCGGCTAGTCCGGCTTTATGCAGTGGTCACCCAGGAACCCATCTACATCATCAC
  GGAATACATGGAGAACGGGAGCCTAGTAGATTTTCTCAAGACTCCCTCGGGCATCAAGTTGAATGTCAAC
  AAACTTTTGGACATGGCAGCCCAGATTGCAGAGGGCATGGCGTTCATCGAAGAACAGAATTACATCCATC
  GGGACCTGCGCGCCGCCAACATCCTGGTGTCTGACACGCTGAGCTGCAAGATTGCAGACTTTGGCCTGGC
  GCGCCTCATTGAGGACAATGAGTACACGGCCCGGGAGGGGGCCAAATTTCCCATTAAGTGGACAGCACCA
  GAAGCCATTAACTATGGGACCTTCACCATCAAGTCAGACGTGTGGTCCTTCGGGATCTTGCTTACAGAGA
  TCGTCACCCACGGTCGAATCCCTTACCCAGGAATGACCAACCCTGAAGTCATTCAGAACCTGGAGAGAGG
  CTACCGCATGGTGAGACCTGACAACTGTCCGGAAGAGCTGTACCACCTCATGATGCTGTGCTGGAAGGAG
  CGCCCAGAGGACCGGCCCACGTTTGACTACCTTCGGAGTGTTCTGGATGACTTCTTCACAGCCACAGAGG
  GCCAGTACCAGCCCCAGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227319 representing NM_001162432
  Red=Cloning site Green=Tags(s)
  
  MGASELTISGIMGCVCSSNPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPAS
  PLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFF
  KNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITF
  PGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTK
  VAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVN
  KLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAP
  EAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKE
  RPEDRPTFDYLRSVLDDFFTATEGQYQPQP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162432
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001162432.1, NP_001155904.1
• RefSeq Size: 2074
• RefSeq ORF: 1563
• Locus ID: 16818