Itgam (NM_008401) Mouse Tagged ORF Clone

CAT#: MG227324

Itgam (GFP-tagged) - Mouse integrin alpha M (Itgam) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itgam
• Synonyms: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227324 representing NM_008401
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTTAAAGCTCTTCTGGTCACAGCCCTAGCCTTGTGTCATGGCTTCAATCTGGACACTGAACATC
  CCATGACCTTCCAAGAGAATGCAAAAGGCTTTGGACAGAGTGTGGTCCAGCTTGGCGGAACCAGTGTGGT
  TGTTGCAGCCCCCCAGGAGGCAAAGGCTGTTAACCAGACAGGTGCCCTCTACCAGTGTGACTACAGCACA
  AGCCGGTGTCACCCCATCCCCCTGCAAGTACCTCCAGAGGCTGTGAATATGTCCTTGGGCCTGTCCCTGG
  CTGTTTCTACTGTCCCCCAGCAGCTGCTGGCCTGTGGCCCCACGGTGCACCAAAACTGCAAGGAGAATAC
  TTATGTGAATGGATTGTGCTATTTGTTCGGCTCCAACCTGCTGAGGCCGCCCCAGCAGTTCCCAGAGGCT
  CTCAGAGAATGTCCTCAGCAGGAGAGTGACATTGTCTTCTTGATTGATGGCTCCGGTAGCATCAACAACA
  TTGACTTTCAGAAGATGAAGGAGTTTGTCTCAACTGTGATGGAGCAGTTCAAAAAGTCTAAAACCTTGTT
  CTCTTTGATGCAGTACTCGGACGAGTTCCGGATTCACTTCACCTTCAATGACTTCAAGAGAAACCCTAGC
  CCAAGATCACATGTGAGCCCCATAAAGCAGCTGAATGGGAGGACAAAAACTGCCTCAGGGATCCGGAAAG
  TAGTGAGAGAACTGTTTCACAAAACCAATGGGGCCCGGGAGAATGCTGCGAAGATCCTAGTTGTCATCAC
  AGATGGAGAAAAATTCGGTGATCCCTTGGATTATAAGGATGTCATCCCCGAGGCAGACAGAGCAGGGGTC
  ATTCGCTACGTAATTGGGGTGGGAAATGCCTTCAACAAACCACAGTCCCGCAGAGAGCTCGACACCATCG
  CATCTAAGCCAGCTGGTGAACACGTGTTCCAAGTGGACAACTTTGAAGCCCTGAATACCATTCAGAACCA
  GCTTCAGGAAAAGATCTTTGCAATTGAGGGCACGCAGACAGGAAGTACCAGCTCCTTTGAGCATGAGATG
  TCTCAAGAAGGCTTCAGTGCTTCCATTACCTCTAATGGTCCCTTGCTGGGCTCTGTGGGGAGCTTTGACT
  GGGCAGGTGGAGCCTTCCTGTATACATCGAAGGATAAAGTCACCTTCATCAACACAACCAGAGTGGATTC
  AGACATGAATGATGCTTACCTGGGTTATGCTTCTGCAGTCATCTTGAGGAACCGTGTCCAAAGCTTGGTT
  TTAGGAGCACCTCGGTATCAGCATATTGGCTTGGTGGTGATGTTCAGGGAGAATTTCGGTACCTGGGAGC
  CCCACACTAGCATCAAGGGCAGCCAGATTGGCTCTTATTTTGGGGCCTCCCTTTGCTCTGTGGACATGGA
  CGCTGATGGCAATACCAACTTGATCCTCATTGGGGCCCCTCATTACTATGAGAAGACCCGAGGAGGCCAG
  GTGTCAGTGTGTCCCTTGCCTCGAGGGAGGGCACGGTGGCAGTGTGAAGCTCTTCTCCACGGTGATCAGG
  GTCATCCCTGGGGTCGCTTTGGGGCAGCCCTGACAGTGCTGGGAGACGTGAATGGGGACAAACTGACAGA
  TGTGGCCATTGGGGCCCCAGGAGAGCAGGAGAATCAGGGCGCTGTCTACATTTTTTATGGAGCATCAATA
  GCCAGCCTCAGTGCCTCCCACAGCCAGCGGATCATAGGCGCCCACTTCTCCCCTGGGCTCCAGTACTTCG
  GGCAGTCTCTGAGTGGGGGTAAGGATCTCACAATGGATGGCTTGATGGACCTGGCTGTTGGGGCGCAGGG
  GCATCTTCTGCTGCTCAGAGCCCAGCCGGTGCTGAGACTGGAGGCAACCATGGAATTCAGCCCCAAGAAA
  GTAGCAAGGAGTGTGTTTGCATGTCAAGAACAAGTATTAAAAAACAAGGATGCTGGGGAGGTCAGAGTCT
  GCCTCCGTGTCCGCAAGAACACCAAGGACCGTCTGCGCGAAGGAGATATCCAGAGCACTGTCACTTATGA
  CCTGGCTTTAGACCCTGGCCGCTCACGTATCCGTGCCTTCTTTGATGAGACAAAGAACAACACACGCAGG
  CGCACCCAGGTCTTTGGATTGATGCAGAAATGTGAAACACTGAAGCTAATTTTACCGGACTGCGTGGACG
  ACTCAGTGAGCCCCATCATCCTGCGCCTCAATTATACACTGGTTGGGGAGCCCTTGAGGTCCTTTGGGAA
  CCTCCGACCAGTTCTGGCTATGGATGCTCAGAGGTTCTTCACAGCTATGTTTCCCTTTGAGAAAAATTGC
  GGCAATGACAGCATCTGCCAAGACGATCTCAGCATCACCATGAGTGCCATGGGCTTGGACACTTTGGTGG
  TGGGAGGCCCCCAGGACTTTAACATGAGTGTGACTCTGAGAAATGACGGTGAGGATTCCTACGGGACCCA
  GGTTACCGTCTACTACCCATCTGGCTTATCTTACCGGAAGGATTCAGCAAGCCAGAACCCGCTCACCAAG
  AAGCCTTGGTTTGTAAAGCCTGCTGAGTCCAGCTCTTCTTCTGAAGGGCATGGGGCTCTGAAGAGTACCA
  CCTGGAACATAAACCATCCCATCTTTCCTGCTAATTCTGAGGTCACATTTAATGTCACATTTGATGTGGA
  CTCTCATGCCTCCTTTGGGAACAAACTGCTCCTCAAAGCCATTGTGGCCAGTGAGAACAACATGTCCAGG
  ACCCACAAAACCAAGTTTCAGTTGGAGCTGCCTGTGAAGTACGCCATCTACATGATTGTCACCAGTGATG
  AGAGTTCTATCAGATATCTCAACTTCACGGCTTCAGAGATGACCAGTAAGGTCATACAGCATCAGTACCA
  GTTCAACAACCTGGGCCAGAGGAGCCTCCCTGTCAGTGTAGTCTTCTGGATCCCTGTTCAGATCAACAAT
  GTGACCGTATGGGATCATCCCCAGGTCATCTTCTCCCAGAACCTCTCAAGTGCCTGTCACACTGAGCAGA
  AATCCCCCCCTCACTCCAATTTCCGGGATCAGCTTGAAAGGACCCCAGTGCTGAACTGCTCTGTTGCAGT
  CTGTAAGAGAATTCAGTGTGACCTTCCATCCTTCAACACTCAGGAAATATTCAATGTCACCCTCAAGGGC
  AACCTATCATTTGACTGGTACATCAAGACTTCTCATGGTCACCTCCTGCTTGTGAGCAGCACTGAGATCC
  TGTTTAATGACTCTGCGTTTGCCCTGCTTCCAGGGCAGGAGTCGTATGTGAGGTCTAAGACAGAGACCAA
  AGTGGAGCCATATGAAGTTCACAATCCTGTACCACTCATTGTGGGCAGCTCCATTGGGGGCCTGGTGCTC
  TTGGCTCTCATCACTGCTGGCCTATACAAGCTTGGCTTTTTCAAGCGGCAGTACAAGGACATGATGAATG
  AAGCTGCCCCCCAAGACGCCCCACCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227324 representing NM_008401
  Red=Cloning site Green=Tags(s)
  
  MTLKALLVTALALCHGFNLDTEHPMTFQENAKGFGQSVVQLGGTSVVVAAPQEAKAVNQTGALYQCDYST
  SRCHPIPLQVPPEAVNMSLGLSLAVSTVPQQLLACGPTVHQNCKENTYVNGLCYLFGSNLLRPPQQFPEA
  LRECPQQESDIVFLIDGSGSINNIDFQKMKEFVSTVMEQFKKSKTLFSLMQYSDEFRIHFTFNDFKRNPS
  PRSHVSPIKQLNGRTKTASGIRKVVRELFHKTNGARENAAKILVVITDGEKFGDPLDYKDVIPEADRAGV
  IRYVIGVGNAFNKPQSRRELDTIASKPAGEHVFQVDNFEALNTIQNQLQEKIFAIEGTQTGSTSSFEHEM
  SQEGFSASITSNGPLLGSVGSFDWAGGAFLYTSKDKVTFINTTRVDSDMNDAYLGYASAVILRNRVQSLV
  LGAPRYQHIGLVVMFRENFGTWEPHTSIKGSQIGSYFGASLCSVDMDADGNTNLILIGAPHYYEKTRGGQ
  VSVCPLPRGRARWQCEALLHGDQGHPWGRFGAALTVLGDVNGDKLTDVAIGAPGEQENQGAVYIFYGASI
  ASLSASHSQRIIGAHFSPGLQYFGQSLSGGKDLTMDGLMDLAVGAQGHLLLLRAQPVLRLEATMEFSPKK
  VARSVFACQEQVLKNKDAGEVRVCLRVRKNTKDRLREGDIQSTVTYDLALDPGRSRIRAFFDETKNNTRR
  RTQVFGLMQKCETLKLILPDCVDDSVSPIILRLNYTLVGEPLRSFGNLRPVLAMDAQRFFTAMFPFEKNC
  GNDSICQDDLSITMSAMGLDTLVVGGPQDFNMSVTLRNDGEDSYGTQVTVYYPSGLSYRKDSASQNPLTK
  KPWFVKPAESSSSSEGHGALKSTTWNINHPIFPANSEVTFNVTFDVDSHASFGNKLLLKAIVASENNMSR
  THKTKFQLELPVKYAIYMIVTSDESSIRYLNFTASEMTSKVIQHQYQFNNLGQRSLPVSVVFWIPVQINN
  VTVWDHPQVIFSQNLSSACHTEQKSPPHSNFRDQLERTPVLNCSVAVCKRIQCDLPSFNTQEIFNVTLKG
  NLSFDWYIKTSHGHLLLVSSTEILFNDSAFALLPGQESYVRSKTETKVEPYEVHNPVPLIVGSSIGGLVL
  LALITAGLYKLGFFKRQYKDMMNEAAPQDAPPQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008401
• ORF Size: 3459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008401.2, NP_032427.2
• RefSeq Size: 4679
• RefSeq ORF: 3462
• Locus ID: 16409