Itgam (NM_001082960) Mouse Tagged ORF Clone

CAT#: MG227326

Itgam (GFP-tagged) - Mouse integrin alpha M (Itgam) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itgam
• Synonyms: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227326 representing NM_001082960
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTTAAAGCTCTTCTGGTCACAGCCCTAGCCTTGTGTCATGGCTTCAATCTGGACACTGAACATC
  CCATGACCTTCCAAGAGAATGCAAAAGGCTTTGGACAGAGTGTGGTCCAGCTTGGCGGAACCAGTGTGGT
  TGTTGCAGCCCCCCAGGAGGCAAAGGCTGTTAACCAGACAGGTGCCCTCTACCAGTGTGACTACAGCACA
  AGCCGGTGTCACCCCATCCCCCTGCAAGTACCTCCAGAGGCTGTGAATATGTCCTTGGGCCTGTCCCTGG
  CTGTTTCTACTGTCCCCCAGCAGCTGCTGGCCTGTGGCCCCACGGTGCACCAAAACTGCAAGGAGAATAC
  TTATGTGAATGGATTGTGCTATTTGTTCGGCTCCAACCTGCTGAGGCCGCCCCAGCAGTTCCCAGAGGCT
  CTCAGAGAATGTCCTCAGCAGGAGAGTGACATTGTCTTCTTGATTGATGGCTCCGGTAGCATCAACAACA
  TTGACTTTCAGAAGATGAAGGAGTTTGTCTCAACTGTGATGGAGCAGTTCAAAAAGTCTAAAACCTTGTT
  CTCTTTGATGCAGTACTCGGACGAGTTCCGGATTCACTTCACCTTCAATGACTTCAAGAGAAACCCTAGC
  CCAAGATCACATGTGAGCCCCATAAAGCAGCTGAATGGGAGGACAAAAACTGCCTCAGGGATCCGGAAAG
  TAGTGAGAGAACTGTTTCACAAAACCAATGGGGCCCGGGAGAATGCTGCGAAGATCCTAGTTGTCATCAC
  AGATGGAGAAAAATTCGGTGATCCCTTGGATTATAAGGATGTCATCCCCGAGGCAGACAGAGCAGGGGTC
  ATTCGCTACGTAATTGGGGTGGGAAATGCCTTCAACAAACCACAGTCCCGCAGAGAGCTCGACACCATCG
  CATCTAAGCCAGCTGGTGAACACGTGTTCCAAGTGGACAACTTTGAAGCCCTGAATACCATTCAGAACCA
  GCTTCAGGAAAAGATCTTTGCAATTGAGGGCACGCAGACAGGAAGTACCAGCTCCTTTGAGCATGAGATG
  TCTCAAGAAGGCTTCAGTGCTTCCATTACCTCTAATGGTCCCTTGCTGGGCTCTGTGGGGAGCTTTGACT
  GGGCAGGTGGAGCCTTCCTGTATACATCGAAGGATAAAGTCACCTTCATCAACACAACCAGAGTGGATTC
  AGACATGAATGATGCTTACCTGGGTTATGCTTCTGCAGTCATCTTGAGGAACCGTGTCCAAAGCTTGGTT
  TTAGGAGCACCTCGGTATCAGCATATTGGCTTGGTGGTGATGTTCAGGGAGAATTTCGGTACCTGGGAGC
  CCCACACTAGCATCAAGGGCAGCCAGATTGGCTCTTATTTTGGGGCCTCCCTTTGCTCTGTGGACATGGA
  CGCTGATGGCAATACCAACTTGATCCTCATTGGGGCCCCTCATTACTATGAGAAGACCCGAGGAGGCCAG
  GTGTCAGTGTGTCCCTTGCCTCGAGGGCAGAGGGCACGGTGGCAGTGTGAAGCTCTTCTCCACGGTGATC
  AGGGTCATCCCTGGGGTCGCTTTGGGGCAGCCCTGACAGTGCTGGGAGACGTGAATGGGGACAAACTGAC
  AGATGTGGCCATTGGGGCCCCAGGAGAGCAGGAGAATCAGGGCGCTGTCTACATTTTTTATGGAGCATCA
  ATAGCCAGCCTCAGTGCCTCCCACAGCCAGCGGATCATAGGCGCCCACTTCTCCCCTGGGCTCCAGTACT
  TCGGGCAGTCTCTGAGTGGGGGTAAGGATCTCACAATGGATGGCTTGATGGACCTGGCTGTTGGGGCGCA
  GGGGCATCTTCTGCTGCTCAGAGCCCAGCCGGTGCTGAGACTGGAGGCAACCATGGAATTCAGCCCCAAG
  AAAGTAGCAAGGAGTGTGTTTGCATGTCAAGAACAAGTATTAAAAAACAAGGATGCTGGGGAGGTCAGAG
  TCTGCCTCCGTGTCCGCAAGAACACCAAGGACCGTCTGCGCGAAGGAGATATCCAGAGCACTGTCACTTA
  TGACCTGGCTTTAGACCCTGGCCGCTCACGTATCCGTGCCTTCTTTGATGAGACAAAGAACAACACACGC
  AGGCGCACCCAGGTCTTTGGATTGATGCAGAAATGTGAAACACTGAAGCTAATTTTACCGGACTGCGTGG
  ACGACTCAGTGAGCCCCATCATCCTGCGCCTCAATTATACACTGGTTGGGGAGCCCTTGAGGTCCTTTGG
  GAACCTCCGACCAGTTCTGGCTATGGATGCTCAGAGGTTCTTCACAGCTATGTTTCCCTTTGAGAAAAAT
  TGCGGCAATGACAGCATCTGCCAAGACGATCTCAGCATCACCATGAGTGCCATGGGCTTGGACACTTTGG
  TGGTGGGAGGCCCCCAGGACTTTAACATGAGTGTGACTCTGAGAAATGACGGTGAGGATTCCTACGGGAC
  CCAGGTTACCGTCTACTACCCATCTGGCTTATCTTACCGGAAGGATTCAGCAAGCCAGAACCCGCTCACC
  AAGAAGCCTTGGTTTGTAAAGCCTGCTGAGTCCAGCTCTTCTTCTGAAGGGCATGGGGCTCTGAAGAGTA
  CCACCTGGAACATAAACCATCCCATCTTTCCTGCTAATTCTGAGGTCACATTTAATGTCACATTTGATGT
  GGACTCTCATGCCTCCTTTGGGAACAAACTGCTCCTCAAAGCCATTGTGGCCAGTGAGAACAACATGTCC
  AGGACCCACAAAACCAAGTTTCAGTTGGAGCTGCCTGTGAAGTACGCCATCTACATGATTGTCACCAGTG
  ATGAGAGTTCTATCAGATATCTCAACTTCACGGCTTCAGAGATGACCAGTAAGGTCATACAGCATCAGTA
  CCAGTTCAACAACCTGGGCCAGAGGAGCCTCCCTGTCAGTGTAGTCTTCTGGATCCCTGTTCAGATCAAC
  AATGTGACCGTATGGGATCATCCCCAGGTCATCTTCTCCCAGAACCTCTCAAGTGCCTGTCACACTGAGC
  AGAAATCCCCCCCTCACTCCAATTTCCGGGATCAGCTTGAAAGGACCCCAGTGCTGAACTGCTCTGTTGC
  AGTCTGTAAGAGAATTCAGTGTGACCTTCCATCCTTCAACACTCAGGAAATATTCAATGTCACCCTCAAG
  GGCAACCTATCATTTGACTGGTACATCAAGACTTCTCATGGTCACCTCCTGCTTGTGAGCAGCACTGAGA
  TCCTGTTTAATGACTCTGCGTTTGCCCTGCTTCCAGGGCAGGAGTCGTATGTGAGGTCTAAGACAGAGAC
  CAAAGTGGAGCCATATGAAGTTCACAATCCTGTACCACTCATTGTGGGCAGCTCCATTGGGGGCCTGGTG
  CTCTTGGCTCTCATCACTGCTGGCCTATACAAGCTTGGCTTTTTCAAGCGGCAGTACAAGGACATGATGA
  ATGAAGCTGCCCCCCAAGACGCCCCACCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227326 representing NM_001082960
  Red=Cloning site Green=Tags(s)
  
  MTLKALLVTALALCHGFNLDTEHPMTFQENAKGFGQSVVQLGGTSVVVAAPQEAKAVNQTGALYQCDYST
  SRCHPIPLQVPPEAVNMSLGLSLAVSTVPQQLLACGPTVHQNCKENTYVNGLCYLFGSNLLRPPQQFPEA
  LRECPQQESDIVFLIDGSGSINNIDFQKMKEFVSTVMEQFKKSKTLFSLMQYSDEFRIHFTFNDFKRNPS
  PRSHVSPIKQLNGRTKTASGIRKVVRELFHKTNGARENAAKILVVITDGEKFGDPLDYKDVIPEADRAGV
  IRYVIGVGNAFNKPQSRRELDTIASKPAGEHVFQVDNFEALNTIQNQLQEKIFAIEGTQTGSTSSFEHEM
  SQEGFSASITSNGPLLGSVGSFDWAGGAFLYTSKDKVTFINTTRVDSDMNDAYLGYASAVILRNRVQSLV
  LGAPRYQHIGLVVMFRENFGTWEPHTSIKGSQIGSYFGASLCSVDMDADGNTNLILIGAPHYYEKTRGGQ
  VSVCPLPRGQRARWQCEALLHGDQGHPWGRFGAALTVLGDVNGDKLTDVAIGAPGEQENQGAVYIFYGAS
  IASLSASHSQRIIGAHFSPGLQYFGQSLSGGKDLTMDGLMDLAVGAQGHLLLLRAQPVLRLEATMEFSPK
  KVARSVFACQEQVLKNKDAGEVRVCLRVRKNTKDRLREGDIQSTVTYDLALDPGRSRIRAFFDETKNNTR
  RRTQVFGLMQKCETLKLILPDCVDDSVSPIILRLNYTLVGEPLRSFGNLRPVLAMDAQRFFTAMFPFEKN
  CGNDSICQDDLSITMSAMGLDTLVVGGPQDFNMSVTLRNDGEDSYGTQVTVYYPSGLSYRKDSASQNPLT
  KKPWFVKPAESSSSSEGHGALKSTTWNINHPIFPANSEVTFNVTFDVDSHASFGNKLLLKAIVASENNMS
  RTHKTKFQLELPVKYAIYMIVTSDESSIRYLNFTASEMTSKVIQHQYQFNNLGQRSLPVSVVFWIPVQIN
  NVTVWDHPQVIFSQNLSSACHTEQKSPPHSNFRDQLERTPVLNCSVAVCKRIQCDLPSFNTQEIFNVTLK
  GNLSFDWYIKTSHGHLLLVSSTEILFNDSAFALLPGQESYVRSKTETKVEPYEVHNPVPLIVGSSIGGLV
  LLALITAGLYKLGFFKRQYKDMMNEAAPQDAPPQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001082960
• ORF Size: 3462 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001082960.1, NP_001076429.1
• RefSeq Size: 4682
• RefSeq ORF: 3465
• Locus ID: 16409