Ldha (NM_001136069) Mouse Tagged ORF Clone

CAT#: MG227451

Ldha (GFP-tagged) - Mouse lactate dehydrogenase A (Ldha) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ldha
• Synonyms: l7R2; Ldh1; Ldhm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227451 representing NM_001136069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGTCCTCAGGCGGCTACACGTACACGGAGACCTCGGTATTATTTTTCCATTTCAAGGTCTCAA
  AAGATTCAAAGTCCAAGATGGCAACCCTCAAGGACCAGCTGATTGTGAATCTTCTTAAGGAAGAGCAGGC
  TCCCCAGAACAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCTTGTGCCATCAGTATCTTAATG
  AAGGACTTGGCGGATGAGCTTGCCCTTGTTGACGTCATGGAAGACAAACTCAAGGGCGAGATGATGGATC
  TCCAGCATGGCAGCCTCTTCCTTAAAACACCAAAAATTGTCTCCAGCAAAGACTACTGTGTAACTGCGAA
  CTCCAAGCTGGTCATTATCACCGCGGGGGCCCGTCAGCAAGAGGGGGAGAGCCGGCTCAACCTGGTCCAG
  CGAAACGTGAACATCTTCAAGTTCATCATTCCCAACATTGTCAAGTACAGTCCACACTGCAAGCTGCTGA
  TCGTCTCCAATCCAGTGGATATCTTGACCTACGTGGCTTGGAAAATCAGTGGCTTTCCCAAAAACCGAGT
  AATTGGAAGTGGTTGCAATCTGGATTCAGCGCGGTTCCGTTACCTGATGGGAGAGAGGCTGGGGGTTCAC
  GCGCTGAGCTGTCACGGCTGGGTCCTGGGAGAACATGGCGACTCCAGTGTGCCTGTGTGGAGTGGTGTGA
  ATGTTGCCGGCGTCTCCCTGAAGTCTCTTAACCCAGAACTGGGCACTGACGCAGACAAGGAGCAGTGGAA
  GGAGGTTCACAAGCAGGTGGTGGACAGTGCCTACGAGGTGATCAAGCTGAAAGGTTACACATCCTGGGCC
  ATTGGCCTCTCTGTGGCAGACTTGGCTGAGAGCATAATGAAGAACCTTAGGCGGGTGCATCCCATTTCCA
  CCATGATTAAGGGTCTCTATGGAATCAATGAGGATGTCTTCCTCAGTGTCCCATGTATCCTGGGACAAAA
  TGGAATCTCGGATGTTGTGAAGGTGACACTGACTCCTGAGGAAGAGGCCCGCCTGAAGAAGAGCGCAGAC
  ACCCTCTGGGGAATCCAGAAGGAGCTGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227451 representing NM_001136069
  Red=Cloning site Green=Tags(s)
  
  MSKSSGGYTYTETSVLFFHFKVSKDSKSKMATLKDQLIVNLLKEEQAPQNKITVVGVGAVGMACAISILM
  KDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSKDYCVTANSKLVIITAGARQQEGESRLNLVQ
  RNVNIFKFIIPNIVKYSPHCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVH
  ALSCHGWVLGEHGDSSVPVWSGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWA
  IGLSVADLAESIMKNLRRVHPISTMIKGLYGINEDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSAD
  TLWGIQKELQF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136069
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001136069.2, NP_001129541.2
• RefSeq Size: 1854
• RefSeq ORF: 1086
• Locus ID: 16828
• Gene Summary: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]