Dph3 (BC029910) Mouse Tagged ORF Clone

CAT#: MR200155

Dph3 (Myc-DDK-tagged) - Mouse DPH3 homolog (KTI11, S. cerevisiae) (cDNA clone MGC:36233 IMAGE:4920779)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dph3
• Synonyms: DelgipP1, Desr1, DELGIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200155 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGTTTCACGACGAGGTGGAGATCGAGGACTTTCAATATGACGAGGACTCGGAGACATATTTCT
  ACCCTTGCCCCTGTGGGGATAACTTTGCCATCACCAAGGAAGATTTGGAAAATGGAGAAGATGTGGCCAC
  GTGTCCTAGCTGCTCACTCATTATAAAAGTGATTTATGACAAAGATCAGTTCATGTGTGGAGAAACAGTC
  CCAGCACCTTCAACCAACAAGGAGTTAGTTAAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200155 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVFHDEVEIEDFQYDEDSETYFYPCPCGDNFAITKEDLENGEDVATCPSCSLIIKVIYDKDQFMCGETV
  PAPSTNKELVKC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC029910
• ORF Size: 246 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029910, AAH29910
• RefSeq Size: 677 bp
• RefSeq ORF: 248 bp
• Locus ID: 105638
• MW: 9.3 kDa
• Gene Summary: Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2. [UniProtKB/Swiss-Prot Function]